Reappraisal of Human HOG and MO3.13 Cell Lines as a Model to Study Oligodendrocyte Functioning
Abstract
:1. Introduction
2. Materials and Methods
2.1. General Reagents
2.2. HOG and MO3.13 Cell Culture and Differentiation
2.3. Co-Culture Experiments
2.4. Glucose and Serum Deprivation Experiments With HOG and MO3.13
2.5. RNA Isolation and cDNA Synthesis
2.6. Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)
2.7. Immunocytochemistry
2.8. Western Blot Analysis
2.9. Statistical Analysis
3. Results
3.1. Differentiation of HOG Cells Does Not Induce a MyOL Expression Profile
3.2. Differentiation of MO3.13 Cells Does Not Induce a MyOL Expression Profile
3.3. Cell Density Affects mRNA Expression in HOG and MO3.13 Cells
3.4. Co-Cultures of HOG or MO3.13 Cells with Neuronal SH-SY5Y Cells Do Not Display Myelin Formation
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Differentiation Medium | Components |
---|---|
N2.1 medium | Eagle’s Minimum Essential Medium (EMEM) 1% antibiotic-antimycotic (AA), 50 μg/mL apo-transferrin, 16 μg/mL putrescine, 0.5 μg/mL human insulin, 30 nM sodium selenite, 30 nM triiodothyronine (T3), 500 uM 3-isobutyl-1-methylxanthine (IBMX), 500 uM dibutyryl cAMP (db-cAMP) |
N2.2 medium | EMEM 1% AA, 50 μg/mL apo-transferrin, 16 μg/mL putrescine, 0.5 μg/mL human insulin, 30 nM sodium selenite, 30 nM T3, 10 ng/mL D-biotin, 50 nM hydrocortisone, 4 μM progesterone |
T3 medium | EMEM 1% AA, 30 nM T3 |
PMA medium | Gibco Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F12(1:1)) 1% AA, 100 nM phorbol 12-myristate 13-acetate (PMA) |
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De Kleijn, K.M.A.; Zuure, W.A.; Peijnenborg, J.; Heuvelmans, J.M.; Martens, G.J.M. Reappraisal of Human HOG and MO3.13 Cell Lines as a Model to Study Oligodendrocyte Functioning. Cells 2019, 8, 1096. https://doi.org/10.3390/cells8091096
De Kleijn KMA, Zuure WA, Peijnenborg J, Heuvelmans JM, Martens GJM. Reappraisal of Human HOG and MO3.13 Cell Lines as a Model to Study Oligodendrocyte Functioning. Cells. 2019; 8(9):1096. https://doi.org/10.3390/cells8091096
Chicago/Turabian StyleDe Kleijn, Kim M. A., Wieteke A. Zuure, Jolien Peijnenborg, Josje M. Heuvelmans, and Gerard J. M. Martens. 2019. "Reappraisal of Human HOG and MO3.13 Cell Lines as a Model to Study Oligodendrocyte Functioning" Cells 8, no. 9: 1096. https://doi.org/10.3390/cells8091096
APA StyleDe Kleijn, K. M. A., Zuure, W. A., Peijnenborg, J., Heuvelmans, J. M., & Martens, G. J. M. (2019). Reappraisal of Human HOG and MO3.13 Cell Lines as a Model to Study Oligodendrocyte Functioning. Cells, 8(9), 1096. https://doi.org/10.3390/cells8091096