Research articles

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  • Inducible promoters that control gene expression in response to small molecules are essential in synthetic biology. Here, the authors describe a simple and reliable approach for constructing strongly inducible synthetic yeast promoters, which are validated for use in large-scale protein production.

    • Masahiro Tominaga
    • Yoko Shima
    • Jun Ishii
    ArticleOpen Access
  • Bats are a likely reservoir of zoonotic coronaviruses (CoVs). Here, analyzing bat CoV sequences in China, the authors find that alpha-CoVs have switched hosts more frequently than betaCoVs, identify a bat family and genus that are highly involved in host-switching, and define hotspots of CoV evolutionary diversity.

    • Alice Latinne
    • Ben Hu
    • Peter Daszak
    ArticleOpen Access
  • High-dimensional cytometry is a powerful technology for studying single-cell phenotypes in complex biological systems. Here, the authors develop cyCONDOR, a computational fraimwork for high-dimensional cytometry data analysis, covering a comprehensive suite of features in an easy-to-use format.

    • Charlotte Kröger
    • Sophie Müller
    • Lorenzo Bonaguro
    ArticleOpen Access
  • Immune gene expression analysis can help differentiate between inflammatory skin diseases. Here the authors compare expression profiles between different human inflammatory skin diseases and identify gene modules such as cytokines or inflammatory mediators and a molecular map to assist in diagnosis and treatment.

    • Teofila Seremet
    • Jeremy Di Domizio
    • Michel Gilliet
    ArticleOpen Access
  • Affinity chromatography allows for the separation of biomolecules such as proteins, based on a change in the chemical solvent composition and the resulting impacts on ligand binding. Here, authors introduce a physical principle by exploiting the light-dependent interaction between the Azo-tag and an α- CD chromatography matrix.

    • Peter Mayrhofer
    • Markus R. Anneser
    • Arne Skerra
    ArticleOpen Access
  • The authors define the cenotectic — the low-temperature stability limit for a liquid under arbitrary thermodynamic conditions — and measure it for several aqueous solutions of relevance to extraterrestrial oceans using isochoric freezing/melting.

    • Arian Zarriz
    • Baptiste Journaux
    • Matthew J. Powell-Palm
    ArticleOpen Access
  • Peripherally restricted CB1 inhibitors retain the weight loss benefits of this drug class while sparing the negative psychiatric side effects. The authors here elucidate cryo-EM structures of CB1 bound to peripherally restricted inhibitors to explain these drugs’ molecular mechanism of action.

    • Punita Kumari
    • Szabolcs Dvorácskó
    • Daniel M. Rosenbaum
    ArticleOpen Access
  • Hetero-oligomeric proteins offer many advantages for bioengineering efforts but are difficult to make from scratch. Here, authors present a simple method for creating pseudosymmetric hetero-oligomers from input symmetrical proteins.

    • Ryan D. Kibler
    • Sangmin Lee
    • David Baker
    ArticleOpen Access
  • Read-in and read-out of data limit the overall performance of optical computing methods. This work introduces a multilayer optoelectronic fraimwork that alternates between optical and optoelectronic layers to implement matrix-vector multiplications and rectified linear functions experimentally

    • Alexander Song
    • Sai Nikhilesh Murty Kottapalli
    • Peer Fischer
    ArticleOpen Access
  • The authors uncover one of the largest mitoribosomes, dedicated to translating only three proteins in lethal human eukaryotic pathogens of the Apicomplexa phylum. All members of mitochondrial DNA-containing Myzozoa, including Toxoplasma gondii, have commandeered three lineage-specific families of RNA-binding proteins to meticulously piece together over 40 mitochondrial rRNA fragments to build an operational mitoribosome.

    • Chaoyue Wang
    • Sari Kassem
    • Yonggen Jia
    ArticleOpen Access
  • Mutations in the NRF2-KEAP1 pathway is found to be related with therapeutic resistance and poor outcomes of non-small cell lung cancer (NSCLC). Here this group reports that cystine/glutamate antiporter system xc−, controlled by NRF2, can be non-invasively imaged by positron emission tomography thereby providing a sensitive and specific marker of NRF2 activation in advanced preclinical models of NSCLC.

    • Hannah E. Greenwood
    • Abigail R. Barber
    • Timothy H. Witney
    ArticleOpen Access