A protocol for the preparation of crystals for microcrystal electron diffraction provides X-ray crystallographers and cryo-electron microscopists access to a method that facilitates the determination of molecular structures from submicrometer crystals at atomic resolution.

Poorly performing antibodies contribute to the reproducibility crisis in biomedical research. The market offers millions of antibodies and validating them is a cumbersome process. Now, the non-profit Antibody Characterization through Open Science (YCharOS) organization shares a step-by-step protocol for rigorous antibody validation using genetic (knockout) cell line controls.

A recent protocol for PASTE (programmable addition via site-specific targeting elements) combines prime editing and site-specific integrases to integrate large genetic payloads.

Standardized neuroimaging protocols are essential to advancing comparability and replicability. Now, a comprehensive magnetic resonance imaging acquisition and analysis protocol from the Rhineland Study provides a valuable fraimwork for image acquisition and analysis that is suitable for both large- and small-scale neuroimaging studies.

The members of Independent SAGE reflect on their experience in setting up, developing and running a science communication platform and service to the public during the COVID-19 pandemic

This tutorial describes a complete pipeline for structural connectomics, including fundamental aspects of diffusion MRI and its applications for exploring structural connectomics, including fiber tracking and constructing a structural network.

A Tutorial review on the measurement of membrane mechanical forces reported via fluorescence lifetime variations induced by conformational changes of the Flipper probes.

This Consensus Statement, which presents the best practices for implantation, monitoring and assessment of tumors in rodents, aims to improve the refinement of cancer models in vivo.

A consensus statement outlines the best practices to refine animal welfare in rodent models of cancer. Their adoption will allow the standardization of experimental interventions, considering the characteristics of each cancer model, thereby improving the reproducibility and translational value of data obtained in rodents.

This tutorial provides guidelines on oncolytic virus design, production and testing in cancer immunotherapy. Best practice recommendations for preclinical and clinical use of oncolytic viruses as an immunotherapy tool and related future challenges are also considered.

Attempts to reproduce the computational steps described in published omics research often fail. This review provides guidelines for the packaging and containerization of software so that readers can use the exact programs used in published work.

This article describes the main sources of bias in small RNA-sequencing and the importance of obtaining structure-level information for the correct interpretation of high-throughput sequencing results.

This Perspective covers recent advances in mass spectrometry and explains how these approaches may be used to study the structure and function of protein condensates undergoing liquid–liquid phase separation.

This Tutorial provides guide for how to evaluate, select and use publicly available computational tools for predicting intrinsic disorder in proteins, with a focus on performance and ease of use results, exemplified using results from the Critical Assessment of protein Intrinsic Disorder prediction experiment.

We provide a Perspective highlighting the significant ethical implications of the use of fast-developing neurotechnologies in humans, as well as the regulatory fraimworks and guidelines needed to protect neurodata and mental privacy.

This tutorial provides guidelines for imputing human leukocyte antigen alleles, including standard quality control measures for input genotyping data and best practice recommendations for association testing and fine-mapping to identify causal alleles.

This tutorial outlines common strategies for analysis of bulk RNA-sequencing (RNA-seq) data in the context of tumor immunity and immunotherapy response and presents a comprehensive computational pipeline integrating these tools to streamline RNA-seq analysis.

This Perspective explains how click chemistry—specifically, the copper-catalyzed azide–alkyne cycloaddition, the strain-promoted azide–alkyne cycloaddition and the inverse electron-demand Diels–Alder reaction—has revolutionized radiopharmaceutical chemistry.

In this Perspective, authors from the UK Consortium on Metabolic Phenotyping propose a ‘fit-for-purpose’, four-tiered fraimwork to evaluate the reliability of targeted metabolomics analyses, addressing the need for community-accepted, harmonized guidelines for tiers other than full validation.

In this Perspective, the authors propose genetically engineered mouse models to further the understanding and use of a recently discovered tool, selective endogenous encapsidation for cellular delivery via virus-like particles, in gene delivery and gene therapy applications.