Content-Length: 21574 | pFad | http://www.ncbi.nlm.nih.gov/pubmed/9492191
earCellular responses of a group of cattle immunized subcutaneously with a low dose of Mycobacterium bovis bacille Calmette-Guerin vaccine (BCG) were measured in vitro and compared with nonimmunized control animals. PBMC taken from immunized animals proliferated and produced IFN-gamma in the presence of M. bovis BCG culture filtrate proteins. The addition of PBMC from immunized animals to M. bovis BCG-infected autologous macrophages also resulted in secretion of IFN-gamma. In contrast, the responses of PBMC from control animals were comparatively low over the period of study. In experiments to study the interaction of non-adherent lymphocytes with infected macrophages, M. bovis BCG growth was inhibited in cultures containing autologous PBMC from immunized and non-immunized control animals. The degree of inhibition was related to lymphocyte concentration but did not correlate with IFN-gamma production. Treatment of macrophages with recombinant IFN-gamma prior to, or postinfection did not alter the intracellular growth kinetics of mycobacteria. It appears, therefore, that although M. bovis BCG immunization of cattle stimulates the generation of a T cell-mediated immune response to M. bovis BCG, the cattle may already possess a high level of innate resistance to M. bovis BCG that requires the presence of lymphocytes.
Fetched URL: http://www.ncbi.nlm.nih.gov/pubmed/9492191
Alternative Proxies: