Papers by Abdelfattah Beltagy
Journal of Bioscience and Applied Research
Background: Although monosodium glutamate (MSG) is commonly used as a food additive, the applicat... more Background: Although monosodium glutamate (MSG) is commonly used as a food additive, the application of higher doses or prolonged uses significantly leads to accumulations in living cells and finally produces cellular toxicity. Persea Americana (avocado) has recently gained substantial popularity and is often marketed as a "superfood" because of its unique nutritional composition, antioxidant content, and biochemical profile. Aim: To evaluate the potential ameliorative role of avocado fruit extract against MSG-induced nephrotoxicity in pregnant rats and their offspring. Thirty-two (24 females and 8 males) albino rats were used in this study. After an acclimatization period of two weeks; the animals were mated, and the pregnant rats were randomly divided into four groups; control (G1), avocado (G2): they were supplemented with 50 mg/kg b.w. of avocado fruit extract, MSG (G3): they were given 3g / kg b.w. of MSG, every other day, and MSG &Avocado (G4): they were given an oral dose of MSG alternatively with avocado fruit extract. At the end of weaning, the female rats and their offspring were sacrificed and the blood was collected and the kidneys were excised to evaluate the renal biochemical and histopathological, and immunohistochemical investigations. Results: In MSG-treated mothers' rats, the renal cortical sections displayed severe histopathological lesions including little renal corpuscles, atrophied glomeruli, and relatively wide Bowman ' s space. However, the offspring displayed mild renal histopathological lesions compared with their mothers. The immunohistochemical results revealed strong PCNA and Bax expression in the renal tissues of MSG-exposed mother rats and their offspring if compared with the control. Furthermore, the mean percentage value of positively expressed cells for caspase-3 appeared significantly higher in the renal cells of MSG-induced mother's rats and their offspring if compared with the control. Additionally, the levels of serum antioxidants (SOD&CAT) and potassium ions appeared significantly lowered while the level of MDA, urea, and creatinine appeared significantly higher if compared with the control. Cosupplementation of avocado fruit extract to MSG-induced mothers rats and their pups successfully alleviated the histopathological, immune-histo-chemical, apoptotic as well as biochemical changes caused by MSG. Conclusion: Avocado fruit extract has a powerful ameliorative role against MSG-induced renal toxicity in mother rats and their offspring.
Delta University Scientific Journal
Monosodium glutamate (MSG) and aspartame are the most food addaitives allover-the world. Prolonge... more Monosodium glutamate (MSG) and aspartame are the most food addaitives allover-the world. Prolonged xposure to the two additives results in several adverse effects on the differnet body organs. Recently, natural palnts and their fruits are considered as a new medical approach for alleviating of many diseases. In the curunt work we attempt to evaluate the possible ameliorative role of Opuntia ficus-indica Fruit against adverse effects of MSG and aspartame on the liver of mother's rats and their offspring. 36 pregnant rats were used in these studies, they were classified into 6 groups(six for each); control, MSG, aspartame, MSG+ Opuntia ficus-indica Fruit, Aspartame+ Opuntia ficus-indica Fruit and MSG+Aspartame + Opuntia ficus-indica Fruit. At the end of weaning (21 day postnatal), the mothers rats and and their pups were sacrificed, the blood was collected and the whole liver was removed to investigate the histopathological and biochemical changes induced in the liver. The obtained results revealed severe histopathological signs in the liver of MSG and aspartame supplemented mother's rats and their pups, these signs included dilated hepatic sinosoids, dialted central veins, pyknotic hepatocytes and presence of multiple Kupffer cells. Also, remarkable apoptosis appeared in the hepatocytes that indicated by strong expression of P53 and weak expression for Bcl-2. An obvious increase in the liver enzymes (ALT&AST) and caspase-3 but significant decrease in the levels of antioxidants (SOD&CAT) were observed in MSG and aspartame supplemented mother's rats and their pups. Suppelementaion of Opuntia ficusindica Fruit juice was successfully ameliorated the deleterious histopathological and biochemical alterations induced by MSG and aspartame. This amelioration was mainly attributed to the vital phytochemical and antioxidant nutrients in this fruit.
This work is mainly designed to evaluate the potential therapeutic role of garlic extract against... more This work is mainly designed to evaluate the potential therapeutic role of garlic extract against cisplatin induced nephrotoxicity in pregnant rats and their offspring. Twenty-four pregnant rats were used in the current study. They were randomly allocated into four equal groups (n=6): control, garlic, and CP + Garlic group. At the end of the weaning period, the mothers and the offsprings of all groups were sacrificed, the kidneys were immediately excised, and processed for histological and biochemical investigations. Also, blood samples were withdrawn and processed for estimation of the assigned biochemical parameters. The renal histological sections from CP-treated mother rats displayed pronounced histopathological lesions however; their offsprings showed mild renal histopatological lesions if compared with those of their mothers. The levels of renal tissue SOD), CAT and GPx enzymes were significantly decreased .On the contrary, the levels of MDA, serum urea and creatinine were sig...
Breast Cancer: Targets and Therapy
Aim: To evaluate the potential therapeutic role of Annona muricata (graviola) fruit and bee venom... more Aim: To evaluate the potential therapeutic role of Annona muricata (graviola) fruit and bee venom (BV) against N-methylnitrosourea (MNU)-induced breast cancer in pregnant female rats and complications in the ovaries. Methods: A total of 24 female rats were induced with a single dose of MNU (50 mg/kg body weight). After confirmation of positive tumor marker, female rats were placed with the males for mating. The pregnant rats were randomly divided into four groups (n=6): MNUinduced only (group 1), MNU-induced rats and supplemented with A. muricata 200 mg/kg diet (group 2), MNU-induced and treated with two doses of BV 75 μg/kg (group 3), and MNU-induced and treated with both A. muricata and BV (group 4). Results: In group 1, the breast tissue of mothers revealed pronounced cellular hyperplasia and histopathological signs. Also, the ovarian tissue of mothers and their offspring displayed deleterious histological changes. In groups 2 and 4, histopathological signs and cellular hyperplasia markedly disappeared in breast tissue. However, the histopathological signs induced by MNU in the ovarian tissue reversed to normal in groups 2-4. Also in groups 2-4, levels of serum MMP1, NFκB, and TNFα significantly decreased, and serum caspase 3 significantly increased either in mother rats or their offspring compared to the MNU-alone group. Levels of serum MDA significantly decreased; however, levels of serum antioxidants (CAT and SOD) significantly increased in all groups 2-4 compared to MNU-alone group. Conclusion: A. muricata has a more powerful therapeutic role than BV against MNU-induced breast cancer in rats; however, both have a powerful ameliorative role against ovarian histopathological alterations induced by MNU. Such ameliorative effects of A. muricata and BV are mainly attributed to their antioxidant, anti-inflammatory, and antiproliferative constituents.
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Papers by Abdelfattah Beltagy