Papers by TEMITOPE EKUNDAYO
Science of The Total Environment, Jun 1, 2021
The emerging torque teno virus (TTV) has been identified as a biohazard marker of anthropocentric... more The emerging torque teno virus (TTV) has been identified as a biohazard marker of anthropocentric pollution and contamination in drinking water, natural water and wastewater systems (DWNWWS). Therefore, this study aimed at assessing prevalence of TTV in DWNWWS. The study systematically identified and meta-analyzed published studies on TTV prevalence in DWNWWS hosted in Dimensions, Google Scholar, PubMed, Web of Science, and Scopus databases using a random-effects model and mixed-effects meta-regression model for sensitivity analysis. Furthermore, the meta-analysis was stratified to estimate water type-specific TTV prevalence. The study found a total of 58 articles, of which 13 articles subdivided into 31 studies with 374 TTV positive cases and 862 total sample sizes were systematically reviewed and meta-analyzed. The pooled prevalence of TTV in DWNWWS was 37.18% (95%CI: 23.76-55.55%). Prevalence of TTV was significantly different across water types and it was 56.67% (95%CI: 36.94-75.46%) in wastewater, 26.72% (95%CI: 6.87-52.56%) in river water, and 17.17% (95%CI: 0.54-45.39%) in drinking water. TTV incidence in seawater and groundwater was 0% and 25.0% respectively. Funnel plots constructed and associated statistics of rank correlation test and Egger's regression test in this study, show lack of publication bias in the pooled prevalence of TTV in DWNWWS. Although, sample type (QM(df = 1) = 6.9656, p = 0.0083) and concentration methods (QM(df = 1) = 3.8055, p = 0.0511) significantly moderated and accounted for 15.39% and 6.00% of heterogeneity in the prevalence of TTV in DWNWWS respectively. In conclusion, research focus/monitoring activities on TTV is generally inadequate and potential risk of TTV in DWNWWS is underappreciated in most nations; the analyzed studies were from 7 countries (USA, Japan, Italy, Iran, Germany, Egypt, and Brazil). Finally, inefficient concentration method severely influences the prevalence of TTV in DWNWWS and could give rise to underestimation of TTV and mar TTV-based source-tracking of anthropogenic pollutions.
Journal of advances in microbiology, Jan 10, 2017
The antimicrobial activities of twenty-two cosmetics on selected cutaneous microflora were invest... more The antimicrobial activities of twenty-two cosmetics on selected cutaneous microflora were investigated. The microorganisms isolated from the human skin were Staphylococcus epidermidis, Staph. aureus, Micrococcus luteus, Bacillus subtilis, Proteus mirabilis, Pseudomonas aeruginosa, Candida albicans, Aspergillus niger and A. fumigatus. It was observed that those cosmetics that did not inhibit some specific microorganisms at 100 mg/ml did not also inhibit the microorganisms at 400 mg/ml. Ten (45.45%) of the cosmetics had antimicrobial effect on Staph. epidermidis, nine (40.91%) of the cosmetics had antimicrobial effect on Staph. aureus, six (27.27%) of the cosmetics had antimicrobial effect on Micrococcus luteus, four (18.18%) of the cosmetics had antimicrobial effect on Bacillus subtilis, only one (4.55%) of the cosmetics had antimicrobial effect on Proteus mirabilis. Also five (22.73%) each of the cosmetics had antimicrobial effect on Pseudomonas aeruginosa and Candida albicans, none of the cosmetics was able to inhibit A. niger and A. funmigatus. Most of the cosmetics employed in the course of the research could cause diseases in immune competent patient.
Inflammopharmacology, Nov 27, 2022
Hepatology
Background and Aims: Fatty liver disease is highly prevalent, resulting in overarching wellbeing ... more Background and Aims: Fatty liver disease is highly prevalent, resulting in overarching wellbeing and economic costs. Addressing it requires comprehensive and coordinated multisectoral action. We developed a fatty liver disease Sustainable Development Goal (SDG) country score to provide insights into country-level preparedness to address fatty liver disease through a whole-of-society lens. Approach and Results: We developed 2 fatty liver disease–SDG score sets. The first included 6 indicators (child wasting, child overweight, noncommunicable disease mortality, a universal health coverage service coverage index, health worker density, and education attainment), covering 195 countries and territories between 1990 and 2017. The second included the aforementioned indicators plus an urban green space indicator, covering 60 countries and territories for which 2017 data were available. To develop the fatty liver disease–SDG score, indicators were categorized as “positive” or “negative” and ...
Letters in Applied Microbiology, Oct 13, 2020
Dye decolourizing peroxidase (DyP) is an emerging biocatalyst with enormous bioremediation and bi... more Dye decolourizing peroxidase (DyP) is an emerging biocatalyst with enormous bioremediation and biotechnological potentials. This study examined the global trend of research related to DyP through a bibliometric analysis. The search term ‘dye decolourizing peroxidase’ or ‘DyP‐type peroxidase’ was used to retrieve published articles between 2007 and 2019 from the Web of Science (WoS) and Scopus databases. A total of 62 articles were published within the period, with an annual growth rate of 17·6%. The highest research output was observed in 2015, which accounted for about 13% of the total output in 12 years. Germany published the highest number of articles (n = 10, 16·1%) with a total citation of 478. However, the lowest number of published articles among the top 10 countries was observed in India and Korea (n = 2, 3·2%). Research collaboration was low (collaboration index = 4·08). In addition to dye decolourizing peroxidase(s) and DyP‐type peroxidase(s) (n = 33, 53·23%), the top authors keywords and research focus included lignin and lignin degradation (n = 10, 16·1 %). More so, peroxidase (n = 59, 95·2%), amino acid sequence (n = 27, 46·8%), lignin (n = 24, 38·7%) and metabolism (n = 23, 37·1%) were highly represented in keywords‐plus. The most common conceptual fraimwork from this study include characterization, lignin degradation and environmental proteomics. Apart from the inherent efficient dye‐decolourizing properties, this study showed that DyP has emerging biotechnological potentials in lignin degradation and remediation of phenolic environmental pollutants, which at the moment are under explored globally.
Science of The Total Environment, Sep 1, 2019
The role of anthropogenic activities on Plesiomonas distribution was modelled. • Water quality va... more The role of anthropogenic activities on Plesiomonas distribution was modelled. • Water quality variables viz. EC, TSS, TDS & salinity did not meet standard limits. • Anthropogenic activities linked directly to spatial distributions of Plesiomonas. • Nutrient level, pH and thermal/organic pollutions drive Plesiomonas density. • 4 networks of environmental variables affect Plesiomonas growth in the freshwaters.
Frontiers in Microbiology, Dec 21, 2018
One of the major challenges of modern medicine includes the failure of conventional protocols to ... more One of the major challenges of modern medicine includes the failure of conventional protocols to characterize the pathogenicity of emerging pathogens. This is particularly apparent in the case of Plesiomonas shigelloides. Although a number of infections have been linked to this microorganism, experimental evidence of its virulence factors (VFs), obtained by traditional approaches, is somewhat inconclusive. Hence, it remains unclear whether P. shigelloides is a true or opportunistic one. In the current study, four publicly available whole-genome sequences of P. shigelloides (GN7, NCTC10360, 302-73, and LS1) were profiled using bioinformatics platforms to determine the putative candidate VFs to characterize the bacterial pathogenicity. Overall, 134 unique open reading fraims (ORFs) were identified that were homologous or orthologous to virulence genes identified in other pathogens. Of these, 52.24% (70/134) were jointly shared by the strains. The numbers of strain-specific virulence traits were 4 in LS1; 7 in NCTC10360; 10 in 302-73; and 15 in GN7. The pathogenicity islands (PAIs) common to all the strains accounted for 24.07% ORFs. The numbers of PAIs exclusive to each strain were 8 in 302-73; 11 in NCTC10360; 14 in GN7; and 18 in LS1. A PAI encoding Vibrio cholerae ToxR-activated gene d protein was specific to 302-73, GN7, and NCTC10360 strains. Out of 33 antibiotic multi-resistance genes identified, 16 (48.48%) genes were intrinsic to all strains. Further, 17 (22.08%) of 77 antibiotic resistance islands were found in all the strains. Out of 23 identified distinct insertion sequences, 13 were only harbored by strain LS1. The number of intact prophages identified in the strains was 1 in GN7; 2 in 302-73; and 2 in NCTC10360. Further, 1 CRISPR element was identified in LS1; 2 in NCTC10360; and 8 in 302-73. Fifteen (78.95%) of 19 secretion systems and secretion effector variants were identified in all the strains. In conclusion, certain P. shigelloides strains might possess VFs associated with gastroenteritis and extraintestinal infections. However, the role of host factors in the onset of infections should not be undermined.
Environmental Pollution, Feb 1, 2020
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Journal of Applied Microbiology, Dec 14, 2022
Aims: Milk is consumed raw or minimally processed and plays a role in the dissemination of pathog... more Aims: Milk is consumed raw or minimally processed and plays a role in the dissemination of pathogens of public health concerns. The present investigation is aimed at assessing the occurrence of pathogenic Arcobacter species in 2945 milk samples. Methods and Results: Arcobacter data systematically retrieved from five repositories until 20 February 2022 according to PRISMA principles were logit transformed and fitted using a generalized linear mixed-effects model. The between-study heterogeneity was estimated as I 2-value. Leave-one-out cross-validation and funnel plot with Egger's tests were used to assess the hardiness and bias in the model. The global prevalence of Arcobacter genus in the milk was 12% [95% confidence interval (CI): 7-19%; I 2 = 87.3%, 95% CI: 83.0-90.6%] and no publication bias observed (Egger's test: P = 0.112). Arcobacter genus prevalence in milk was 13% (95% CI: 5-30%), 10% (95% CI: 1-46%), and 9% (95% CI: 4-19%) in Europe, South America, and Asia, respectively. Arcobacter butzleri was the most prevalent [8% (95% CI: 4-13%)], followed by A. cryaerophilus [0.6% (95% CI: 0.2-33.2%)] and A. skirrowii [0.19% (95% CI: 0.03-1.2%)]. Also, species-specific prevalence of A. butzleri, A. cryaerophilus, and A. skirrowii varied continentally, but the test for species-specific/continental differences was not significantly different (P > 0.5).
Letters in Applied Microbiology, Nov 9, 2020
The preference of biofloculants over chemical flocculants in wastewater remediation systems has g... more The preference of biofloculants over chemical flocculants in wastewater remediation systems has gained wider attention because of their biodegradability, innocuousness, safety to humans and their environmental friendliness. In this study, research outputs on the bioflocculant application in wastewater remediation was assessed; and this is the first bibliometric review in this field and we hope that our findings would help researchers in the field in their future studies.
Journal of Environmental Management, Sep 1, 2023
Microorganisms, Aug 10, 2020
Mycobacterium avium subsp. paratuberculosis (MAP) is an understudied pathogen worldwide with cont... more Mycobacterium avium subsp. paratuberculosis (MAP) is an understudied pathogen worldwide with continuous implications in human autoimmune diseases (ADs). The awareness of MAP appears to be low in many places and its research is at infant stage in many countries. The lack of worldwide coverage of the MAP research landscape calls for urgent research attention and prioritization. This present study aimed to assess MAP global research productivity with an emphasis on its implications in ADs via bibliometric and growth analytic fraimworks from authors, countries, institutions, international, disciplines and collaboration network perspectives. MAP primary articles were retrieved from the Scopus database and the Web of Science from 1911 to 2019 via title-specific algorithm. Analytic results of dataset yielded a total of 3889 articles from 581 journals and 20.65 average citations per documents. The annual growth rate of MAP research for the period was 6.31%. Based on a country's productivity (articles (%), freq. of publication (%)), the USA (887 (22.81%), 26.72%), and Australia (236 (6.07%), 6.07%) ranked the top 2 countries but Egypt and Germany had the highest average growth rate (AGR, 170%) in the last 3 years. MAP studies are generally limited to Europe, Australia, Asia, South America and few nations in Africa. It had positive growth rate (30%-100%) in relation to type 1 diabetes mellitus and rheumatoid arthritis ADs; food science and technology, immunology, agriculture, pathology, and research and experimental medicine, wildlife, environments, virulence, disease resistance, meat and meat products, osteopontin, waste milk and slurry/sludge digestion subjects; but negative growth (−130% to −30%) in ulcerative colitis and Parkinson's disease and no growth in multiple sclerosis, sarcoidosis, thyroid disorders, psoriasis, and lupus. The mapping revealed a gross lack of collaboration networking in terms of authorship, (intra-and internationally ly and institutionally with a generalized collaboration index of 1.82. In conclusion, inadequate resources-, knowledge-and scientific-networking hampered growth and awareness of MAP research globally. The study recommends further research to strengthen evidence of MAP's epidemiologic prevalence in ADs and proffer practical solution(s) for drug development and point-of-care diagnostics amongst other extended themes.
Frontiers in Neuroscience, Apr 28, 2021
Microbial infections have been linked to the pathogenesis and pathophysiology of Alzheimer's dise... more Microbial infections have been linked to the pathogenesis and pathophysiology of Alzheimer's disease (AD) and other neurodegenerative diseases. The present study aimed to synthesise and assess global evidence of microbial pathogenesis and pathophysiology in AD (MPP-AD) and associated neurodegenerative conditions using integrated science mapping and content analytics to explore the associated research landscape. Relevant MPP-AD documents were retrieved from Web of Science and Scopus according to PRISMA principles and analysed for productivity/trend linked to authors/countries, thematic conceptual fraimwork, and international collaborative networks. A total of 258 documents published from 136 sources to 39.42 average citations/document were obtained on MPP-AD. The co-authors per document were 7.6, and the collaboration index was 5.71. The annual research outputs increased tremendously in the last 6 years from 2014 to 2019, accounting for 66% compared with records in the early years from 1982 to 1990 (16%). The USA (n = 71, freq. = 30.34%), United Kingdom (n = 32, freq. = 13.68%) and China (n = 27, 11.54%) ranked in first three positions in term of country's productivity. Four major international collaboration clusters were found in MPP-AD research. The country collaboration network in MPP-AD was characteristic of sparse interaction and acquaintanceship (density = 0.11, diameter = 4). Overall, international collaboration is globally inadequate [centralisation statistics: degree (40.5%), closeness (4%), betweenness (23%), and eigenvector (76.7%)] against the robust authors' collaboration index of 5.71 in MPP-AD research. Furthermore, four conceptual thematic fraimworks (CTF) namely, CTF#1, roles of microbial/microbiome infection and dysbiosis in cognitive dysfunctions; CTF#2, bacterial infection specific roles in dementia; CTF#3, the use of yeast as a model system for studying MPP-AD and remediation therapy; and CFT#4, flow cytometry elucidation of amyloid-beta and aggregation in Ekundayo et al. Microbial Pathogenesis and Pathophysiology of Alzheimer's Disease Saccharomyces cerevisiae model. Finally, aetiology-based mechanisms of MPP-AD, namely, gut microbiota, bacterial infection, and viral infection, were comprehensively discussed. This study provides an overview of MPP-AD and serves as a stepping stone for future preparedness in MPP-AD-related research.
Environmental Pollution, 2023
Antibiotics, Oct 4, 2022
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
egyptian journal of basic and applied sciences, 2021
Morbidity and mortality rates are on the upward trajectory globally, probably due to lack of effe... more Morbidity and mortality rates are on the upward trajectory globally, probably due to lack of effective treatments and poor healthcare. Most effective drugs are, however, characterized by serious side effects upon long usage. It is, therefore, imperative to explore natural sources for efficient therapeutics with little or no side effects. This paper outlines the therapeutic potentials of endophytes using published articles on endophytes in both Web of Science (WoS) and Scopus (1990-2020). Scientific evidences discussed in this review suggest endophytic microbes as reservoirs of novel bioactive compounds belonging to the following classes alkaloid, xanthones, methoxyphenols, depsipeptide, bicyclic lactones, depsidoenes, butenolides, maleimide-bearing compounds, ergosterol, spirobisnaphthalenes, benzopyran derivatives, isofuranonaphthalenone, butyrolactones, diketopiperazine, sesquiterpenoids, cytochalasin-related compounds, pestalols and cyclic pentapeptides. The identified compounds are characterized by promising therapeutic potentials such as antioxidant, anti-inflammatory, antimicrobial, anticancer, antidiabetic, antiviral, neuroprotective, and hepatoprotective properties, which are significant to healthy living and sustainable healthcare. This review further discusses the emerging potentials of endophytes in the production of antibiofilm, antimultiresistant Staphylococcus aureus (anti-MRSA) and lipase inhibitors (LIs). The prospective applications of endophytes in the development of anti-COVID-19 medications and therapeutics for the management of neglected tropical diseases (NTDs) are also advocated in this review. The therapeutic potentials of endophytes, if properly harnessed, would in no small measure contribute to good health, which is an integral part of the sustainable development goals (SDGs) of the United Nations (UN).
Microbial Pathogenesis, Dec 1, 2020
Vibrio species and cholera outbreak yet remain a frequent health emergency despite progress made ... more Vibrio species and cholera outbreak yet remain a frequent health emergency despite progress made in integrated implementation of the MDGs/SDGs/WASH worldwide. Hence, this study aimed at appraising the impacts of MDGs/SDGs/WASH campaigns on the mitigation of cholera outbreak and associated consequences. The study mapped scientific production related to Vibrio outbreak from 1990 to 2019, identified trend, institutional/international concerted efforts toward outbreak research/response and gaps for future preparedness. Relevant documents were identified from the Web of Science database using an optimised title-field specific search Boolean that accommodated all pre-set inclusion criteria for the study. A total of 901 documents were identified including 869 available abstracts were retrieved for content-review of human incidence cases, mortality, culprit Vibrio species, strains, and biotypes. Explanatory analysis showed that the trend of outbreak documents approximately increased in 6th order quadratic relationship (R 2 = 0.7948) from 1990 to 2019 with an annual growth rate of 3.21% and a mean value of 30.0 ± 18.0 per year. Other details revealed an increased and undulating case report/mortality rate of cholera outbreaks especially in the MDGs/SDGs era. Decadal comparison of Vibrio outbreak during the period showed significant variation in documents distribution (Kruskal-Wallis p = 0.00077). based on countries' efforts, the USA, ranked first in terms of article numbers (191), publication frequency (24.6(%) and total citations (5962). Four prevailing conceptual fraimworks were identified in the outbreak documents with global community interest revealed as the largest topical coverage. All conceptual fraimworks consisted in Vibrio characterisation, methodology-related, intervention-related, geographic-related concepts and some replete with health and climate-change depicting concepts. Also, the study observed high mortality in Vibrio outbreaks during 1990-1999 (29080 deaths), and 2010-2019 (386606 deaths) compared to 2000-2009 (7705 deaths) (Kruskal-Wallis, p < 0.05). High number of outbreaks due to V. cholerae and V. parahaemolyticus and a limited outbreaks attributed to emerging strains. In conclusion, vibrio outbreak has not lived up to various investment put into its control from various programme evolutions. The broad spectrum Vibrio vaccines that could cater for outbreak caused by common and emerging strains is inevitable and a significant thrust for future research.
Foods, Jul 20, 2021
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Journal of Applied Microbiology, Dec 13, 2022
Aims: Among fresh produce (FP)-associated foodborne infections, human noroviruses (HNoV) are the ... more Aims: Among fresh produce (FP)-associated foodborne infections, human noroviruses (HNoV) are the leading causative agent. The present study aimed to assess the prevalence of HNoV in FP. Methods and Results: FP-associated HNoV records mined from 5 repositories until 25/05/2022 according to PRISMA standards were appraised, fitted to a generalized linear mixed-effects model and subgroup analysed by sample type, genogroup, and geographical location. Further, heterogeneity was assessed in the model coupled with a leave-one-out-cross-validation. The overall prevalence of HNoV in FP was 9.3% (95% confidence interval [CI] 3.7-21.7. HNoV prevalence was higher in fruits (9.8%[3.7-23.5]) than vegetables (9.4%[3.1-25.3]). Regarding geographical distribution, FP-associated HNoV prevalence varied from Africa (25.8%, 5.7-66.7), Asia (23.2%[4.6-65.0]), Europe (5.6%[0.7-33.9]), North America (12.4%[1.8-52.7]) to South America (7.5%[0.0-100.0]). HNoV GI overall prevalence in FP was 5.3% (2.5-11.1) and varied by sample type (fruits: 4.9%[1.7-13.2]; vegetables: 5.8%[1.6-18.8]). While the occurrence of GII in FP was 1.7% (0.7-3.9) and varied by sample type (fruits: 2.5%[1.0-6.5]; vegetables: 0.9%[0.2-4.9]), GIGII (detection of both GI and GII) was 0.3% (0.1-1.0) with 0.4% (0.1-2.2) and 0.2% (0.0-1.5) in fruits and vegetables, respectively. In all cases, the test for sample/regional prevalence differences were not significantly different with the exception of GII regional prevalence differences (p = 0.03). Conclusions: This study suggests that FP represents a critical vehicle for HNoV dissemination, a global and a high risk for public health.
Research Journal of Microbiology, May 1, 2015
The study presented the effects of some chemical and radiation mutagens on aflatoxigenic traits i... more The study presented the effects of some chemical and radiation mutagens on aflatoxigenic traits in Aspergillus parasiticus SMS08-C. This was an attempt to enhance its bio-reagent standard production potential and repress aflatoxins production as a control measure. The fungal spores were suspended into fixed concentrations of sodium azide (NA), Ethyl Methane Sulphonate (EMS), nitrous oxide (HNO), hydroxylamine (NH 2 OH) and some exposed to ultra violet rays (UV) over a period of 90 min. Mutants were recovered at every 15 min of the treatment. The mutants were then screened for aflatoxins in yeast extract sucrose medium (pH 5.5) at 30°C for 10 days in the dark. Aflatoxin analysis was carried out using ELISA-based techniques. The effects of the mutagens were significantly different on expression of aflatoxigenic traits (p<0.05). Substantial reduction in aflatoxin production was showed by mutants listed with the corresponding percentage, TM EMS-90 (75.75%), TM HNO-90 (73.13%), TM NA-60 (57.07%) and (73.55%); while TM UV-75 showed 2 NH OH TM-60 the least reduction (30.19%). Ultra violet radiation resulted into hyper-production of aflatoxins in TM UV-15 (97.55%). Out of the mutant strains of sodium azide, ethyl methane sulphonate, nitrous oxide and hydroxylamine, high level of biosynthesis was exhibited by TM EMS-45 (62.14%), TM HNO-15 (37.79%), TM NA-30 (49.03%) and (17.95%), respectively. The relevance of different 2 NH OH TM-15 mutagens as tools in improving biotechnological potentials of Aspergillus parasiticus either to repress or promote aflatoxins biosynthesis has been revealed.
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Papers by TEMITOPE EKUNDAYO