Papers by Virgilio Loriato
Embriogênese animal X Embriogênese vegetal •Eixo embrionário •Reversibilidade da programação gené... more Embriogênese animal X Embriogênese vegetal •Eixo embrionário •Reversibilidade da programação genética •Especificação dos órgãos •Reversibilidade parcial? (Dolly) Seqüência de eventos da embriogênese em Arabidopsis. Laux e Jürgens (1997), The Plant Cell, 9. Embriogênese somática Exemplo da teoria da TOTIPOTENCIALIDADE e da PLASTICIDADE do desenvolvimento vegetal. Conceito: É um processo pelo qual, células somáticas haplóides ou diplóides, se diferenciam em plantas completas seguindo estádios de desenvolvimento similares aqueles observados na embriogênese zigótica REG. CRESC. DETERMINATIVO
Plant Cell Tissue and Organ Culture
Root explants of a wild passionfruit species (Passiflora cincinnata) and three P. edulis commerci... more Root explants of a wild passionfruit species (Passiflora cincinnata) and three P. edulis commercial populations (‘FB 100’, ‘FB 200’, and ‘FB 300’) were incubated on Murashige and Skoog (MS) medium supplemented with 4.44 μM 6-benzyladenine (BA) to induce shoot organogenesis. Shoots elongated in liquid medium with 2.89 μM gibberellic acid (GA3) under agitation were rooted in coconut fiber and acclimatized followed by transfer to a greenhouse into pots containing mixture of coconut fiber and Plantmax® (1:1). Explant samples were collected during organogenesis and submitted to light and scanning electron microscopy (SEM). Root explants of P. cincinnata responded earlier than those of P. edulis. However, on the third assessment, at 90 days, the genotype ‘FB 200’ showed shoot number significantly higher than ‘FB 100’ and ‘FB 300’, not differing from P. cincinnata. Organogenesis in P. cincinnata and P. edulis occurred via direct pathway, which was confirmed by anatomical studies and SEM. Flow cytometric analysis revealed no variation in DNA content of regenerated plantlets among all genotypes. Nuclear DNA (2C) values (pg) in regenerants of P. cincinnata (2.99 pg) and P. edulis (3.26–3.28 pg) were consistent with DNA amounts of seed-derived control plants.
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Papers by Virgilio Loriato