Vulvovaginal candidiasis is a common fungal infection that affects the female genital system. Res... more Vulvovaginal candidiasis is a common fungal infection that affects the female genital system. Resistance to antifungals is an emergent problem worldwide. We aimed to detect the prevalence of different Candida species associated with vulvovaginal candidiasis, determine their antifungal susceptibility pattern, and evaluate the molecular mechanisms associated with Fluconazole resistance. Methodology: This study included 300 patients. Candida species have been identified phenotypically. Antifungal susceptibility was tested using a disc diffusion method. The molecular mechanisms of Fluconazole-resistance were determined by analyzing the expression levels of Fluconazole target, efflux pump and efflux pump-regulator genes by
Background and Objectives: Clostridium difficile infection (CDI) has become a significant healthc... more Background and Objectives: Clostridium difficile infection (CDI) has become a significant healthcare-associated infection throughout the world and is particularly important in developing countries. This study aimed to investigate clinical characterization and risk factors related to toxigenic C. difficile infection in adult and pediatric patients, antimicrobial susceptibility pattern. Also, to evaluate different diagnostic methods for rapid detection of C. difficile associated diarrhea (CDAD) in Egypt. Materials and Methods: Stool samples were collected from 95 pediatric patients and 37 adult patients suffering from antibiotic associated diarrhea and were subjected to direct toxin immunoassay and culture on cycloserine/cefoxitin/fructose agar. The presence of tcdA and tcdB genes was tested by PCR. Results: Toxigenic C. difficile was isolated from pediatric and adult patients at a rate of 17.89% (17/95) and 27% (10/37) respectively. The sensitivity and specificity of direct PCR from ...
Vulvovaginal candidiasis is a common fungal infection that affects the female genital system. Res... more Vulvovaginal candidiasis is a common fungal infection that affects the female genital system. Resistance to antifungals is an emergent problem worldwide. We aimed to detect the prevalence of different Candida species associated with vulvovaginal candidiasis, determine their antifungal susceptibility pattern, and evaluate the molecular mechanisms associated with Fluconazole resistance. Methodology: This study included 300 patients. Candida species have been identified phenotypically. Antifungal susceptibility was tested using a disc diffusion method. The molecular mechanisms of Fluconazole-resistance were determined by analyzing the expression levels of Fluconazole target, efflux pump and efflux pump-regulator genes by
Background and Objectives: Clostridium difficile infection (CDI) has become a significant healthc... more Background and Objectives: Clostridium difficile infection (CDI) has become a significant healthcare-associated infection throughout the world and is particularly important in developing countries. This study aimed to investigate clinical characterization and risk factors related to toxigenic C. difficile infection in adult and pediatric patients, antimicrobial susceptibility pattern. Also, to evaluate different diagnostic methods for rapid detection of C. difficile associated diarrhea (CDAD) in Egypt. Materials and Methods: Stool samples were collected from 95 pediatric patients and 37 adult patients suffering from antibiotic associated diarrhea and were subjected to direct toxin immunoassay and culture on cycloserine/cefoxitin/fructose agar. The presence of tcdA and tcdB genes was tested by PCR. Results: Toxigenic C. difficile was isolated from pediatric and adult patients at a rate of 17.89% (17/95) and 27% (10/37) respectively. The sensitivity and specificity of direct PCR from ...
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Papers by rawhia fathy