Abstract
A field trial was conducted at two sites in the savanna ecosystem of eastern Colombia to compare the effects of inoculation with vesicular-arbuscular mycorrhizal fungi (VAMF) ofBrachiaria dictyoneura (a tropical grass), cassava (Manihot esculenta), the tropical forage legume kudzu (Pueraria phaseoloides) andSorghum sp., and two phosphate sources. The second stage of the trial studied the effect of these pre-crop treatments on the subsequent growth, nutrition and VAM status of cowpea (Vigna unguiculata) andStylosanthes capitata in the following season, compared with both crops sown in native savanna.
Inoculation significantly increased the levels of VAM and plant yields in the early growth stages of all crops during the first season, particularly with the rock phosphate (RP) source. The most significant increases were observed in the mycorrhiza-dependent cassava and kudzu crops up to 15 weeks after sowing, and were associated with increased foliar uptake of P and Mg. The effectiveness of the introduced inoculum was greater at the field site with a sandier soil.
In the second season the levels of VAM in roots of cowpea andS. capitata were all increased significantly in pre-cropped plots compared with a savanna control. The increased presence of VAM was associated with significantly increased yields on plots previously sown to cassava, kudzu andSorghum sp. The data support the idea that increasing the VAMF inoculum potential of these acid-infertile soils by inoculation or pre-crops can greatly increase the rate of establishment of mycorrhiza-dependent host plants.
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References
Arias I, Koomen I, Dodd J C and Hayman D S 1990 Growth responses of mycorrhizal and non-mycorrhizal tropical forage species to different levels of soil phosphate (submitted).
Arines J, Vilarino A and Sainz M 1988 ‘Fine’ and ‘coarse’ mycorrhizal fungi on red clover plants in acid soils: Root colonization and plant responses. Plant and Soil 111, 135–145.
Baylis G T S 1975 The magnoloid mycorrhiza and mycotrophy in root systems derived from it.In Endomycorrhizas. Eds. F E Sanders, B Mosse and P B Tinker. pp 373–389. Academic Press, New York and London.
Baltruschat H and Dehne H W 1988 The occurrence of vesicular-arbuscular mycorrhiza in agro-ecosystems. I. Influence of nitrogen fertilization and green manure in continuous monoculture and in crop rotation on the inoculum potential of winter wheat. Plant and Soil 107, 279–284.
Bethlenfalvay G J, Ulrich J M and Brown M S 1985 Plant response to mycorrhizal fungi: Host, endophyte and soil effects. Soil Sci. Soc. Am. J. 49, 1164–1168.
Centro Internacional de Agricultura Tropical (CIAT) 1986a Annual Report 1985. Cassava Program. Cali, Colombia.
Centro Internacional de Agricultural Tropical (CIAT) 1986b Annual Report 1985. Tropical Pastures Program. Cali, Colombia.
Dodd J C and Jeffries P 1986 Early development of vesicular-arbuscular mycorrhizas in autumn-sown cereals. Soil Biol. Biochem. 18, 149–154.
Dodd J C, Arias I, Koomen I and Hayman D S 1990 The management of vesicular-arbuscular mycorrhizal populations in acid-infertile soils of a savanna ecosystem. II. The effects of pre-crops on the spore populations of native and introduced VAM fungi. Plant and Soil 122, 241–247.
Fitter A H 1986 Effect of benomyl on leaf phosphorus concentration in alpine grasslands: A test of mycorrhizal benefit. New Phytol. 103, 767–776.
Fredeen A L and Terry N 1988 Influence of vesicular-arbuscular mycorrhizal infection and soil phosphorus level on growth and carbon metabolism of soybean. Can. J. Bot. 66, 2311–2316.
Ganry F, Diem H G, Wey J and Dommergues Y 1985 Inoculation withGlomus mosseae improves N2 fixation by field-grown soybean. Biol. Fert. Soils 1, 15–23.
Goedert W J 1983 Management of the Cerrado soils of Brazil: A review. J. Soil Sci. 34, 405–428.
Graham J H, Linderman R G and Menge J A 1982 Development of external hyphae by different isolates of mycorrhizalGlomus spp. in relation to root colonization and growth of Troyer citrange. New Phytol. 91, 183–189.
Gueye M, Diem H G and Dommergues Y R 1987 Variation in N2 fixation, N and P contents of mycorrhizalVigna unguiculata in relation to the progressive development of extraradical hyphae ofGlomus mosseae. MIRCEN J. 3, 75–86.
Hammond L L, Chien S H and Mokwunye A U 1986 Agronomic value of unacidulated and partially acidulated phosphate rocks indigenous to the tropics. Adv. Agron. 40, 89–140.
Harinikumar K H and Bagyaraj D J 1988 Effect of crop rotation on native vesicular-arbuscular mycorrhizal propagules in soil. Plant and Soil 110, 77–80.
Hayman D S, Johnson A M and Ruddlesdin I 1975 The influence of phosphate and crop species onEndogone spores and vesicular-arbuscular mycorrhiza under field conditions. Plant and Soil 43, 489–495.
Hayman D S 1984 Methods for evaluating and manipulating vesicular-arbuscular mycorrhiza.In Microbiological Methods for Environmental Biotechnology. Eds. J M Lynch and J M Grainger. p 95–117. Academic Press, London.
Howeler R H, Sieverding E and Saif S 1987 Practical aspects of mycorrhizal technology in some tropical crops and pastures. Plant and Soil 100, 249–283.
Ilag L L, Rosales A M, Elazegui F A and Mew T W 1987 Changes in the population of infective endomycorrizal fungi in a rice-based cropping system. Plant and Soil 103, 67–73.
Islam R, Ayanaba A and Sanders F E 1980 Response of cowpea (Vigna unguiculata) to inoculation with VA-mycorrhizal fungi and to rock phosphate fertilization in some unsterilized Nigerian soils. Plant and Soil 54, 107–117.
Kormanik P P, Bryan W G and Schultz R C 1980 Procedures and equipment for staining large numbers of plant root samples for endomycorrhizal assay. Can. J. Microbiol. 26, 536–538.
Kruckelmann H W 1975 Effects of fertilizers, soils, soil tillage and plant species on the frequency of Endogone chlamydospores and mycorrhizal infection in arable soils.In Endomycorrhizas. Eds. F E Sanders, B Mosse and P B Tinker. pp 511–525. Academic Press, London.
Miranda J C C 1982 Influencia de fungos endomicorrizicos inoculados a campo na cultura de sorgo e soja em um solo sob cerrado. R. bras. Ci. Solo. 6, 19–23.
Mosse B 1977 The role of mycorrhiza in legume nutrition on marginal soils.In Exploiting the Legume-Rhizobium Symbiosis in Tropical Agriculture. Eds. J M Vincent et al. pp 275–292 University of Hawaij, Niftal Project.
Newbould P and Rangeley 1984 Effect of lime, phosphorus and mycorrhizal fungi on growth, nodulation and nitrogen fixation by white clover (Trifolium repens) grown in UK hill soils. Plant and Soil 76, 105–114.
Newman E I, Child R D and Patrick C M 1986 Mycorrhizal infection in grasses of Kenyan savanna. J. Ecol. 74, 1179–1183.
Olsen S R, Watanabe F S and Danielson R E 1961 Phosphorus absorption by corn roots as affected by moisture and phosphorus concentration. Soil Sci. Soc. Am. Proc. 25, 289–294.
Phillips J M and Hayman D S 1970 Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Trans. Brit. Mycol. Soc. 55, 158–161.
Saif S R 1986 Vesicular-arbuscular mycorrhizae in tropical forage species as influenced by season, soil texture, fertilizers, host species and ecotypes. Angew. Botanik. 60, 125–139.
Saif S R 1987 Growth responses of tropical forage plant species to vesicular-arbuscular mycorrhizae. I. Growth, mineral uptake and mycorrhizal dependency. Plant and Soil 97, 25–35.
Salinas J G, Sanz J I and Sieverding E 1985 Importance of VA mycorrhizae for phosphorus supply to pasture plants in tropical oxisols. Plant and Soil 84, 347–360.
Sanders F E, Tinker P B, Black R L B and Palmerley S M 1977 The development of endomycorrhizal root systems. I. Spread of infection and growth-promoting effects with four species of vesicular-arbuscular endophyte. New Phytol. 78, 257–268.
Sieverding E 1986 Research model towards practical application of VA mycorrhizal fungi in tropical agriculture.In Physiological and Genetic Aspects of Mycorrhizae. Eds. V Gianinazzi-Pearson and S Gianinazzi. pp 475–478. Proc. 1st Eur. Symp. Mycorr. Dijon: INRA.
Sieverding E and Howeler R H 1985 Influence of species of VA mycorrhizal fungi on cassava yield response to phosphorus fertilization. Plant and Soil 88, 213–221.
Sylvester-Bradley R, Mosquera D and Mendez J E 1988 Inhibition of nitrate accumulation in tropical grassland soils: Effect of nitrogen fertilization and soil disturbance. J. Soil Sci. 39, 407–416.
Thompson J P 1987 Decline of vesicular-arbuscular mycorrhizae in long fallow disorder of field crops and its expression in phosphorus deficiency of Sunflower. Aust. J. Agric. Res. 38, 847–867.
Toledo M J and Nores A G 1986 Tropical pasture technology for marginal lands of tropical America. Outl. Agric. 15, 2–9.
Wallace L L and Svejcar T 1987 Mycorrhizal and clipping effects onAndropogon gerardii photosynthesis. Am. J. Bot. 74, 1138–1142.
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Dodd, J.C., Arias, I., Koomen, I. et al. The management of populations of vesicular-arbuscular mycorrhizal fungi in acid-infertile soils of a savanna ecosystem. Plant Soil 122, 229–240 (1990). https://doi.org/10.1007/BF02851980
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DOI: https://doi.org/10.1007/BF02851980