Abstract
A novel homologue of insect defensin designated lucifensin (Lucilia defensin) was purified from the extracts of various tissues (gut, salivary glands, fat body, haemolymph) of green bottle fly (Lucilia sericata) larvae and from their excretions/secretions. The primary sequence of this peptide of 40 residues and three intramolecular disulfide bridges was determined by ESI-QTOF mass spectrometry and Edman degradation and is very similar to that of sapecin and other dipteran defensins. We assume that lucifensin is the key antimicrobial component that protects the maggots when they are exposed to the highly infectious environment of a wound during the medicinal process known as maggot therapy. We also believe that lucifensin is that long-sought larger molecular weight antimicrobial factor of the Lucilia sericata excretions/secretions believed to be effective against pathogenic elements of the wound microbial flora.
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Acknowledgments
This work was supported by Grant No. 203/08/0536 of the Czech Science Foundation and by Research Project No. Z40550506 of the Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic. We thank our technical assistants Mrs. Jarmila Titzenthalerová and Mrs. Jitka Čermáková for help with the breeding the files and dissections of larval organs. We also thank Gale A. Kirking at English Editorial Services, s.r.o. for assistance with the English.
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Čeřovský, V., Žďárek, J., Fučík, V. et al. Lucifensin, the long-sought antimicrobial factor of medicinal maggots of the blowfly Lucilia sericata . Cell. Mol. Life Sci. 67, 455–466 (2010). https://doi.org/10.1007/s00018-009-0194-0
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DOI: https://doi.org/10.1007/s00018-009-0194-0