Abstract
Soil microbial communities mediate critical ecosystem carbon and nutrient cycles. How microbial communities will respond to changes in vegetation and climate, however, are not well understood. We reciprocally transplanted soil cores from under oak canopies and adjacent open grasslands in a California oak–grassland ecosystem to determine how microbial communities respond to changes in the soil environment and the potential consequences for the cycling of carbon. Every 3 months for up to 2 years, we monitored microbial community composition using phospholipid fatty acid analysis (PLFA), microbial biomass, respiration rates, microbial enzyme activities, and the activity of microbial groups by quantifying 13C uptake from a universal substrate (pyruvate) into PLFA biomarkers. Soil in the open grassland experienced higher maximum temperatures and lower soil water content than soil under the oak canopies. Soil microbial communities in soil under oak canopies were more sensitive to environmental change than those in adjacent soil from the open grassland. Oak canopy soil communities changed rapidly when cores were transplanted into the open grassland soil environment, but grassland soil communities did not change when transplanted into the oak canopy environment. Similarly, microbial biomass, enzyme activities, and microbial respiration decreased when microbial communities were transplanted from the oak canopy soils to the grassland environment, but not when the grassland communities were transplanted to the oak canopy environment. These data support the hypothesis that microbial community composition and function is altered when microbes are exposed to new extremes in environmental conditions; that is, environmental conditions outside of their “life history” envelopes.
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References
Abraham, WR, Hesse, C, Pelz, O (1998) Ratios of carbon isotopes in microbial lipids as an indicator of substrate usage. Appl Environ Microbiol 64: 4202–4209
Allen, MF, Morris, SJ, Edwards, F, Allen, EB (1995) Microbe–plant interactions in Mediterranean-type habitats: shifts in fungal symbiotic and saprophytic functioning in response to global change. In: Moreno JM, Oechel WC (Eds.) Global Change and Mediterranean-type Ecosystems, Ecological Studies, Springer-Verlag, New York, pp 287–305
Arao, T (1999) In situ detection of changes in soil bacterial and fungal activities by measuring 13C incorporation into soil phospholipid fatty acids from 13C acetate. Soil Biol Biochem 31: 1015–1020
Balser, TC, Firestone, MK (2005) Linking microbial community composition and soil processes in a California annual grassland and a mixed-conifer forest. Biogeochemistry 73: 395–415
Bardgett, RD, Kandeler, E, Tscherko, D, Hobbs, PJ, Bezemer, TM, Jones, TH, Thompson, LJ (1999) Below-ground microbial community development in a high temperature world. Oikos 85: 193–203
Bever, JD (1994) Feedback between plants and their soil communities in an old field community. Ecology (Tempe) 75: 1965–1977
Bossio, DA, Scow, KM (1995) Impact of carbon and flooding on the metabolic diversity of microbial communities in soils. Appl Environ Microbiol 61: 4043–4050
Bossio, DA, Scow, KM, Gunapala, N, Graham, KJ (1998) Determinants of soil microbial communities: effects of agricultural management, season, and soil type on phospholipid fatty acid profiles. Microb Ecol 36: 1–12
Buckley, DH, Schmidt, TM (2001) The structure of microbial communities in soil and the lasting impact of cultivation. Microb Ecol 42: 11–21
Broughton, LC, Gross, KL (2000) Patterns of diversity in plant and soil microbial communities along a productivity gradient in a Michigan old-field. Oecologia (Berlin) 125: 420–427
Canals, RM, Herman, DJ, Firestone, MK (2003) How disturbance by fossorial mammals alters N cycling in a California annual grassland. Ecology 84: 875–881
Cavigelli, MA, Robertson, GP (2000) The functional significance of denitrifier community composition in a terrestrial ecosystem. Ecology 81: 1402–1414
Eviner, VT, Chapin, FS (2003) Gopher-plant-fungal interactions affect establishment of an invasive grass. Ecology 84: 120–128
Felske, A, Wolterink, A, Van Lis, R, De Vos, WM, Akkermans, ADL (2000) Response of a soil bacterial community to grassland succession as monitored by 16s rRNA levels of the predominant ribotypes. Appl Environ Microbiol 66: 3998–4003
Fierer, N, Schimel, JP (2002) Effects of drying–rewetting frequency on soil carbon and nitrogen transformations. Soil Biol Biochem 34: 777–787
Downie, DE, Taskey, RD (1997) Soil characteristics of blue oak and coast live oak ecosystems. USDA Forest Service Gen. Tech. Rep. PSW-GTR-160, pp 65–73
Gastine, A, Scherer-Lorenzen, M, Leadley, PW (2003) No consistent effects of plant diversity on root biomass, soil biota and soil abiotic conditions in temperate grassland communities. Appl Soil Ecol 24: 101–111
Gulledge, J, Schimel, JP (1998) Moisture control over atmospheric CH4 consumption and CO2 production in diverse Alaskan soils. Soil Biol Biochem 30: 1127–113
Herman, DJ, Halverson, LJ, Firestone, MK (2003) Nitrogen dynamics in an annual grassland: oak canopy, climate, and microbial population effects. Ecol Appl 13: 593–604
Jackson, LE, Strauss, RB, Firestone, MK, Bartolome, JW (1990) Influence of tree canopies on grassland productivity and nitrogen dynamics in deciduous oak savanna. Agric Ecosyst Environ 32: 89–105
Kampichler, C, Kandeler, E, Bardgett, RD, Jones, TH, Thomson, LJ (1998) Impact of elevated CO2 concentration on soil microbial biomass and activity in a complex, weedy, field model ecosystem. Global Change Biol 4: 335–346
Kandeler, E, Tscherko, D, Bardgett, RD, Hobbs, PJ, Kampichler, C, Jones, TH (1998) The response of soil microorganisms and roots to elevated CO2 and temperature in a terrestrial model ecosystem. Plant Soil 202: 251–262
Kowalchuk, GA, Buma, DS, de Boer, W, Klinkhamer, PGL, van Veen, JA (2002) Effects of above-ground plant species composition and diversity on the diversity of soil-borne microorganisms. Antonie van Leeuwenhoek 81: 509–520
Lundquist, EJ, Scow, KM, Jackson, LE, Uesugi, SL, Johnson, CR (1991) Rapid response of soil microbial communities from conventional, low input, and organic farming systems to a wet/dry cycle. Soil Biol Biochem 31: 1661–1675
Miller, M, Palojarvi, A, Rangger, A, Reeslev, M, Kjoller, A (1998) The use of fluorogenic substrates to measure fungal presence and activity in soil. Appl Environ Microbiol 64: 613–617
Ringelberg, DB, Stair, JO, Almeida, J, Norby, RJ, O'Neill, EG, White, DC (1997) Consequences of rising atmospheric carbon dioxide levels for the belowground microbiota associated with white oak. J Environ Qual 26: 495–503
Schimel, JP, Gulledge, JM, Clein-Curley, JS, Lindstrom, JE, Braddock, JF (1999) Moisture effects on microbial activity and community structure in decomposing birch litter in the Alaskan taiga. Soil Biol Biochem 31: 831–838
Stark, JM, Firestone, MK (1996) Kinetic characteristics of ammonium-oxidizer communities in a California oak woodland–annual grassland. Soil Biol Biochem 28: 1307–1317
Waldrop, MP, Firestone, MK (2004) Altered utilization patterns of young and old soil C by microorganisms caused by temperature shifts and N additions. Biogeochemistry 67: 235–248
Waldrop, MP, Firestone, MK (in press) Seasonal dynamics of microbial community composition and function in oak canopy and grassland soils. Microb Ecol
White, DC, Ringelberg, DB (1998) Signature lipid biomarker analysis. In: Burlage RS, Atlas R, Stahl D, Geesey G, Sayler G (Eds.) Techniques in Microbial Ecology, Oxford University Press, New York, pp 255–272
Zelles, L, Bai, QY (1994) Fatty acid patterns of phospholipids and lipopolysaccharides in environmental samples. Chemosphere 28: 391–411
Acknowledgment
We want to thank the Hopland Research and Extension Center and Charles Vaughn for support of this project, and David Harris and the University of California at Davis isotope facility for the use of their instrumentation. This work was made possible by financial support from the Kearney Foundation for Soil Science Research and California AES project 6117-H.
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Waldrop, M.P., Firestone, M.K. Response of Microbial Community Composition and Function to Soil Climate Change. Microb Ecol 52, 716–724 (2006). https://doi.org/10.1007/s00248-006-9103-3
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DOI: https://doi.org/10.1007/s00248-006-9103-3