Biology bulletin of the Russian Academy of Sciences, 2017
⎯Endangered freshwater pearl mussels Margaritifera margaritifera reveal a complex life cycle with... more ⎯Endangered freshwater pearl mussels Margaritifera margaritifera reveal a complex life cycle with an obligate host-dependent phase. Only two species, Atlantic salmon Salmo salar and brown trout S. trutta, are important hosts in Europe, indicating a high degree of specialization. Whilst freshwater pearl mussels with their filtering activity can provide important ecosystem services and indirectly improve the habitat quality for their salmonid hosts, their direct effects on physiological stress, reduced swimming performance, and increased mortality at high rates of infestation all support a parasitic character of the mussel during its hostdependent phase. From an evolutionary perspective, both the much greater generation time of the parasite compared to the host, as well as the great distribution ranges of M. margaritifera and its hosts should favour local adaptation patterns. The variable suitability of different salmonid strains and species as hosts for M. margaritifera and the resulting differences in the performance of larvae during the host-dependent phase indicate that host-management strategies should focus on maintaining high quality hosts at a regional scale to avoid selection or genetic drift effects which could erode the genetic and evolutionary potential for adaptation to changing environmental conditions.
Zenodo (CERN European Organization for Nuclear Research), Sep 13, 2019
Modeling of fish population developments in the context of hydropower impacts and restoration pla... more Modeling of fish population developments in the context of hydropower impacts and restoration planning requires autecological information on critical life stages (especially on juvenile stages and reproduction). We compiled and examined the current data availability in peer-reviewed and grey literature on autecological requirements of ten rheophilic fish species at risk, belonging to the salmonid, cyprinid, and cottid families. In total, 1725 data points from 223 sources were included. Economically important salmonids and the common nase were the most studied species. Grey and peer-reviewed data showed similar dispersion and variance and contributed nearly equally to the data pool of the specific species. An in-depth analysis on seven ecological parameters revealed no significant differences between both sources in terms of data availability and quality. We found substantial deficits in the data for about a quarter of the reviewed ecological parameters, in particular on individual densities in the habitats, egg development and information about juvenile stages despite the necessity of such data for more advanced population analyses. To secure fish populations in the long term, more data on basic autecological parameters is needed and grey literature might add valuable information, particularly if it relies on standardized methodologies.
. The hyporheic zone (HZ) is of major importance for carbon and nutrient cycling as well as for t... more . The hyporheic zone (HZ) is of major importance for carbon and nutrient cycling as well as for the ecological health of stream ecosystems. However, biogeochemical observations in this ecotone are complicated by a very high spatial heterogeneity and temporal dynamics. Especially the latter are difficult to observe without disturbing the system. In this field study, we tested and combined three less common methods for time-resolved measurements with high vertical resolution. We installed Rhizon samplers for repeated pore-water extraction, an optical sensor unit for in-situ measurements of dissolved oxygen, and a depth-resolved temperature monitoring system in the HZ of a small stream. While Rhizon samplers were found to be highly suitable for pore-water sampling of dissolved solutes, measured gas concentrations, here CH4, showed a strong dependency of the pump rate during sample extraction, and an isotopic shift in gas samples became evident. This was presumably caused by a different behaviour of water and gas phase in the pore-space. The manufactured oxygen-sensor could locate the oxic-anoxic interface with very high precision. This is ecologically important and allows to distinguish aerobic and anaerobic processes. Temperature data could not only be used to estimate vertical hyporheic exchange, but also depicted sedimentation and erosion processes. Overall, the combined approach was found to be a promising tool to acquire data for the quantification of biogeochemical processes in the HZ with high spatial and temporal resolution.
Since historical times, the inherent human fascination with pearls turned the freshwater pearl mu... more Since historical times, the inherent human fascination with pearls turned the freshwater pearl mussel Margaritifera margaritifera (Linnaeus, 1758) into a highly valuable cultural and economic resource. Although pearl harvesting in M. margaritifera is nowadays residual, other human threats have aggravated the species conservation status, especially in Europe. This mussel presents a myriad of rare biological features, e.g. high longevity coupled with low senescence and Doubly Uniparental Inheritance of mitochondrial DNA, for which the underlying molecular mechanisms are poorly known. Here, the first draft genome assembly of M. margaritifera was
Aquatic Conservation-marine and Freshwater Ecosystems, Jun 9, 2021
The global sustainable development goals of the United Nations (United Nations, 2015) and the ass... more The global sustainable development goals of the United Nations (United Nations, 2015) and the associated necessary transformations, such as energy decarbonization (Sachs et al., 2019), have resulted in increased hydropower development around the world (Zarfl et al., 2015). Advocates of hydropower point to the important contributions of this form of energy to the reduction of CO 2 emissions, its low cost, and its use as a stable source of energy, justifying it as a 'green' energy that makes an important contribution to electricity supply compared with other forms of regenerative energy. For the year 2018, the International Energy Agency (IEA) lists a global energy supply from hydropower of 4,325,111 GWh, compared with 1,273,409 GWh for wind energy and 554,382 GWh for solar/photovoltaic energy (IEA, 2020). In contrast to wind or solar power, hydropower production is available at any time of day and is less dependent on current weather conditions. According to the Collins Dictionary (www.collinsdictionary.com, accessed 14 February 2021), 'green energy' is defined as 'power that comes from sources that do not harm the environment …'. The debate about how 'green' the green energies really are is controversial (Gibson, Wilman & Laurance, 2017). Opponents of hydropower production often refer to it as a 'red' energy because of the mortalities and injuries that fish face when passing turbines (Mueller, Pander & Geist, 2017; Mueller et al., 2020b), as well as other ecological harm to free-flowing rivers associated with habitat fragmentation and alteration.
bioRxiv (Cold Spring Harbor Laboratory), Jun 6, 2021
The long-term evolutionary impacts of whole genome duplication (WGD) are strongly influenced by t... more The long-term evolutionary impacts of whole genome duplication (WGD) are strongly influenced by the ensuing rediploidization process. Following autopolyploidization, rediploidization involves a transition from tetraploid to diploid meiotic pairing, allowing duplicated genes (ohnologues) to diverge genetically and functionally. Our understanding of autopolyploid rediploidization has been informed by a WGD event ancestral to salmonid fishes, where large genomic regions are characterized by temporally delayed rediploidization, allowing lineage-specific ohnologue sequence divergence in the major salmonid clades. Here, we investigate the long-term outcomes of autopolyploid rediploidization at genome-wide resolution, exploiting a recent 'explosion' of salmonid genome assemblies, including a new genome sequence for the huchen (Hucho hucho). We developed a genome alignment approach to capture duplicated regions across multiple species, allowing us to create 121,864 phylogenetic trees describing ohnologue divergence across salmonid evolution. Using molecular clock analysis, we show that 61% of the ancestral salmonid genome experienced an initial 'wave' of rediploidization in the late Cretaceous (85-106 Mya). This was followed by a period of relative genomic stasis lasting 17-39 My, where much of the genome remained in a tetraploid state. A second rediploidization wave began in the early Eocene and proceeded alongside species diversification, generating predictable patterns of lineage-specific ohnologue divergence, scaling in complexity with the number of speciation events. Finally, using gene set enrichment, gene expression, and codon-based selection analyses, we provide insights into potential functional outcomes of delayed rediploidization. Overall, this study enhances our understanding of delayed autopolyploid rediploidization and has broad implications for future studies of WGD events. .
Canadian Journal of Fisheries and Aquatic Sciences, Sep 1, 2021
Captive breeding programs are widely applied by conservation practitioners as a means of conservi... more Captive breeding programs are widely applied by conservation practitioners as a means of conserving, reintroducing, and supplementing populations of imperilled freshwater fishes and mussels. We conducted a systematic map to provide an overview of the existing literature on the effectiveness of captive breeding and release programs. A key finding is that there is limited evaluation of the effectiveness of such programs at all three stages (i.e., broodstock collection, rearing/ release methods, and post-release monitoring). We identified clusters of evidence for evaluating supplementation associated with rearing/release methods for fish growth and survival metrics, and the monitoring stage for fish genetic diversity, growth, and survival metrics, primarily focused on salmonids. However, many studies had inadequate experimental designs (i.e., lacked a comparator). Overall, there was a paucity of studies on the effectiveness of captive breeding programs for imperilled freshwater mussels, highlighting the need to make such information broadly available when studies are undertaken. Outputs from this systematic map (i.e., the map database and heatmaps) suggest that the effectiveness of captive breeding and release programs requires further systematic evaluation.
2021 20th IEEE International Conference on Machine Learning and Applications (ICMLA), Dec 1, 2021
Climate change results in altered air and water temperatures. Increases affect physicochemical pr... more Climate change results in altered air and water temperatures. Increases affect physicochemical properties, such as oxygen concentration, and can shift species distribution and survival, with consequences for ecosystem functioning and services. These ecosystem services have integral value for humankind and are forecasted to alter under climate warming. A mechanistic understanding of the drivers and magnitude of expected changes is essential in identifying system resilience and mitigation measures. In this work, we present a selection of state-of-the-art Neural Networks (NN) for the prediction of water temperatures in six streams in Germany. We show that the use of methods that compare observed and predicted values, exemplified with the Root Mean Square Error (RMSE), is not sufficient for their assessment. Hence we introduce additional analysis methods for our models to complement the state-of-the-art metrics. These analyses evaluate the NN's robustness, possible maximal and minimal values, and the impact of single input parameters on the output. We thus contribute to understanding the processes within the NN and help applicants choose architectures and input parameters for reliable water temperature prediction models.
The purpose of this study was to mark endangered sterlet (Acipenser ruthenus) with visible implan... more The purpose of this study was to mark endangered sterlet (Acipenser ruthenus) with visible implant elastomer (VIE) in order to assess mortality, compatibility, retention, persistence and histological reactions resulting from this tagging technique. It was hypothesized there would be only minor effects on the fishes' health, and assumed that acute effects would be more pronounced than long-term effects. On 11 September 2013, 20 specimens were tagged ventrally with visible implant elastomer, 20 received a subcutaneous injection with 0.9% NaCl solution, and another 20 served as untreated control. Mean total length was 28.0 ± 1.8 cm and mean body mass 64.1 ± 12.0 g. The sterlets were kept in four 4,000-L tanks filled with 2,400-L water. Acute effects were monitored for 95 days, where fish were held at temperatures between 2.4°C and 15.2°C, reflecting outdoor conditions. Chronic effects were examined 282 days posttagging through histological sections of the tagging region in five sterlets. During the first 95 days of observation, tag retention was 100%. No signs of incompatibility were detected. Body mass did not significantly differ between VIE-tagged fish and controls. At day 282 post-tagging, however, distinct tissue reactions were visible at the tagging sites of nine fish. Histological examination of five fish revealed a variable degree of infiltration with leukocytes in the areas around the elastomer, which did not necessarily correspond with the externally visible degree of inflammation. After medical treatment, the lesions healed without complications, whereas the retention rate of the VIE tags was 5%. According to the findings, the tag location rather than the tag itself was responsible for the externally visible irritations, indicating that the ventral subcutis of sterlet is not a suitable site, even for small VIE tags in long-term studies. The results of this study also suggest that VIE marking should in general be critically evaluated before application in field studies.
Securing adequate supply of high-quality water is of increasing global importance and relies in l... more Securing adequate supply of high-quality water is of increasing global importance and relies in large part on ecosystem services provided by freshwater biota. Unionid mussels are important keystone species and habitat engineers that shape freshwater ecosystems through water filtration, nutrient cycling and provision of habitats; their rapid global declines result in dramatic losses of ecosystem functions. Maintenance and enhancement of the services they provide depend on the identification of their crucial habitats. Following theoretical assumptions, this study analyses the importance of lake-stream transition zones for unionid mussels, based on data collected in 1984 and 2019 from an undisturbed stream flowing through five consecutive lakes. Mussel distribution matched the distribution of host fish and was strongly influenced by lakes: densities were highest near lake outlets, reaching 290 ind. m-2 (14.7 kg m-2) in 2019, and declined with downstream distance following a negative power function. This pattern was spatially consistent and sustained over time. All six unionid species native to north-central Europe were present, but common species (Anodonta anatina, Unio pictorum, U. tumidus) contributed about 80% of individuals and were responsible for most of the ecosystem services provided by unionid mussels. Estimated 1.9 × 106 mussel individuals inhabiting 3.2 km of stream length filtered a water volume equivalent to the total stream discharge approximately 2.5 times daily. Aggregations of spent shells, up to 17 kg m-2, accumulated downstream of lakes, forming extensive shell and mussel beds, providing habitats and contributing shell hash that improved stream-bed conditions. Globally invasive Dreissena polymorpha was present at low densities and did not spread or increase in abundance, indicating a long-term biotic resistance of the natural native community. Our study underscores the importance of undisturbed lake outlets, longitudinal connectivity of riverine ecosystems, and of common mussel species in maintaining freshwater ecosystem functionality and provision of vital services.
Aquatic Conservation: Marine and Freshwater Ecosystems, 2019
The freshwater pearl mussel (Margaritifera margaritifera) is an endangered species in Europe, pro... more The freshwater pearl mussel (Margaritifera margaritifera) is an endangered species in Europe, protected nationally and internationally, but with a steadily declining range and abundance owing to pressures such as pollution, river engineering, and illegal exploitation. Despite this, no consistent approaches have been developed around Europe for monitoring pearl mussel populations and their habitats. To address this need, experts on pearl mussel ecology from 11 countries met at a series of workshops in order to develop a protocol for monitoring, published under the auspices of the European Committee for Standardization (CEN). This standard is unique, as it is the first CEN standard dedicated to a single species of conservation concern. The standard is aimed at scientists, conservation bodies, and environmental regulators, and can be used for designing national monitoring programmes as well as reporting on the conservation status of pearl mussel populations under the European Habitats ...
Zenodo (CERN European Organization for Nuclear Research), Sep 6, 2018
Despite of structural deficits, highly modified water bodies (HMWB) contain remnant populations o... more Despite of structural deficits, highly modified water bodies (HMWB) contain remnant populations of endangered fish that have high conservation value. Restoration in HMWBs underlies different principles when compared to natural rivers because of impaired river dynamic processes. The objective herein was to assess the contribution of restored habitats of the River Günz, Germany to fish diversity and restoration success of target species. All habitat restoration types, including bank habitats, fast flowing habitats with gravel, structured shallow water zones, nature like fish passes, and a former river course below a spillway were assessed. None of them comprised the full set of 27 fish species or size classes, indicating that the diversity and interlinkage of the restored habitats is most crucial to sustainable fish populations and high biodiversity. When considering the rheophilic target species Chondrostoma nasus and Barbus barbus, only fish passes and the former river course with their high flow current contributed much to their population development, particularly to young specimens <20 cm. The results of this study indicate that measures inside the main channel are unlikely to mitigate deficiencies in HMWBs concerning specialized riverine fish. Instead, interlinkage of a diversity of restoration measures and investment of resources for restoration in HMWBs into nature-like fish passes or former river courses below spillways with dynamically managed discharges seem most useful.
Biology bulletin of the Russian Academy of Sciences, 2017
⎯Endangered freshwater pearl mussels Margaritifera margaritifera reveal a complex life cycle with... more ⎯Endangered freshwater pearl mussels Margaritifera margaritifera reveal a complex life cycle with an obligate host-dependent phase. Only two species, Atlantic salmon Salmo salar and brown trout S. trutta, are important hosts in Europe, indicating a high degree of specialization. Whilst freshwater pearl mussels with their filtering activity can provide important ecosystem services and indirectly improve the habitat quality for their salmonid hosts, their direct effects on physiological stress, reduced swimming performance, and increased mortality at high rates of infestation all support a parasitic character of the mussel during its hostdependent phase. From an evolutionary perspective, both the much greater generation time of the parasite compared to the host, as well as the great distribution ranges of M. margaritifera and its hosts should favour local adaptation patterns. The variable suitability of different salmonid strains and species as hosts for M. margaritifera and the resulting differences in the performance of larvae during the host-dependent phase indicate that host-management strategies should focus on maintaining high quality hosts at a regional scale to avoid selection or genetic drift effects which could erode the genetic and evolutionary potential for adaptation to changing environmental conditions.
Zenodo (CERN European Organization for Nuclear Research), Sep 13, 2019
Modeling of fish population developments in the context of hydropower impacts and restoration pla... more Modeling of fish population developments in the context of hydropower impacts and restoration planning requires autecological information on critical life stages (especially on juvenile stages and reproduction). We compiled and examined the current data availability in peer-reviewed and grey literature on autecological requirements of ten rheophilic fish species at risk, belonging to the salmonid, cyprinid, and cottid families. In total, 1725 data points from 223 sources were included. Economically important salmonids and the common nase were the most studied species. Grey and peer-reviewed data showed similar dispersion and variance and contributed nearly equally to the data pool of the specific species. An in-depth analysis on seven ecological parameters revealed no significant differences between both sources in terms of data availability and quality. We found substantial deficits in the data for about a quarter of the reviewed ecological parameters, in particular on individual densities in the habitats, egg development and information about juvenile stages despite the necessity of such data for more advanced population analyses. To secure fish populations in the long term, more data on basic autecological parameters is needed and grey literature might add valuable information, particularly if it relies on standardized methodologies.
. The hyporheic zone (HZ) is of major importance for carbon and nutrient cycling as well as for t... more . The hyporheic zone (HZ) is of major importance for carbon and nutrient cycling as well as for the ecological health of stream ecosystems. However, biogeochemical observations in this ecotone are complicated by a very high spatial heterogeneity and temporal dynamics. Especially the latter are difficult to observe without disturbing the system. In this field study, we tested and combined three less common methods for time-resolved measurements with high vertical resolution. We installed Rhizon samplers for repeated pore-water extraction, an optical sensor unit for in-situ measurements of dissolved oxygen, and a depth-resolved temperature monitoring system in the HZ of a small stream. While Rhizon samplers were found to be highly suitable for pore-water sampling of dissolved solutes, measured gas concentrations, here CH4, showed a strong dependency of the pump rate during sample extraction, and an isotopic shift in gas samples became evident. This was presumably caused by a different behaviour of water and gas phase in the pore-space. The manufactured oxygen-sensor could locate the oxic-anoxic interface with very high precision. This is ecologically important and allows to distinguish aerobic and anaerobic processes. Temperature data could not only be used to estimate vertical hyporheic exchange, but also depicted sedimentation and erosion processes. Overall, the combined approach was found to be a promising tool to acquire data for the quantification of biogeochemical processes in the HZ with high spatial and temporal resolution.
Since historical times, the inherent human fascination with pearls turned the freshwater pearl mu... more Since historical times, the inherent human fascination with pearls turned the freshwater pearl mussel Margaritifera margaritifera (Linnaeus, 1758) into a highly valuable cultural and economic resource. Although pearl harvesting in M. margaritifera is nowadays residual, other human threats have aggravated the species conservation status, especially in Europe. This mussel presents a myriad of rare biological features, e.g. high longevity coupled with low senescence and Doubly Uniparental Inheritance of mitochondrial DNA, for which the underlying molecular mechanisms are poorly known. Here, the first draft genome assembly of M. margaritifera was
Aquatic Conservation-marine and Freshwater Ecosystems, Jun 9, 2021
The global sustainable development goals of the United Nations (United Nations, 2015) and the ass... more The global sustainable development goals of the United Nations (United Nations, 2015) and the associated necessary transformations, such as energy decarbonization (Sachs et al., 2019), have resulted in increased hydropower development around the world (Zarfl et al., 2015). Advocates of hydropower point to the important contributions of this form of energy to the reduction of CO 2 emissions, its low cost, and its use as a stable source of energy, justifying it as a 'green' energy that makes an important contribution to electricity supply compared with other forms of regenerative energy. For the year 2018, the International Energy Agency (IEA) lists a global energy supply from hydropower of 4,325,111 GWh, compared with 1,273,409 GWh for wind energy and 554,382 GWh for solar/photovoltaic energy (IEA, 2020). In contrast to wind or solar power, hydropower production is available at any time of day and is less dependent on current weather conditions. According to the Collins Dictionary (www.collinsdictionary.com, accessed 14 February 2021), 'green energy' is defined as 'power that comes from sources that do not harm the environment …'. The debate about how 'green' the green energies really are is controversial (Gibson, Wilman & Laurance, 2017). Opponents of hydropower production often refer to it as a 'red' energy because of the mortalities and injuries that fish face when passing turbines (Mueller, Pander & Geist, 2017; Mueller et al., 2020b), as well as other ecological harm to free-flowing rivers associated with habitat fragmentation and alteration.
bioRxiv (Cold Spring Harbor Laboratory), Jun 6, 2021
The long-term evolutionary impacts of whole genome duplication (WGD) are strongly influenced by t... more The long-term evolutionary impacts of whole genome duplication (WGD) are strongly influenced by the ensuing rediploidization process. Following autopolyploidization, rediploidization involves a transition from tetraploid to diploid meiotic pairing, allowing duplicated genes (ohnologues) to diverge genetically and functionally. Our understanding of autopolyploid rediploidization has been informed by a WGD event ancestral to salmonid fishes, where large genomic regions are characterized by temporally delayed rediploidization, allowing lineage-specific ohnologue sequence divergence in the major salmonid clades. Here, we investigate the long-term outcomes of autopolyploid rediploidization at genome-wide resolution, exploiting a recent 'explosion' of salmonid genome assemblies, including a new genome sequence for the huchen (Hucho hucho). We developed a genome alignment approach to capture duplicated regions across multiple species, allowing us to create 121,864 phylogenetic trees describing ohnologue divergence across salmonid evolution. Using molecular clock analysis, we show that 61% of the ancestral salmonid genome experienced an initial 'wave' of rediploidization in the late Cretaceous (85-106 Mya). This was followed by a period of relative genomic stasis lasting 17-39 My, where much of the genome remained in a tetraploid state. A second rediploidization wave began in the early Eocene and proceeded alongside species diversification, generating predictable patterns of lineage-specific ohnologue divergence, scaling in complexity with the number of speciation events. Finally, using gene set enrichment, gene expression, and codon-based selection analyses, we provide insights into potential functional outcomes of delayed rediploidization. Overall, this study enhances our understanding of delayed autopolyploid rediploidization and has broad implications for future studies of WGD events. .
Canadian Journal of Fisheries and Aquatic Sciences, Sep 1, 2021
Captive breeding programs are widely applied by conservation practitioners as a means of conservi... more Captive breeding programs are widely applied by conservation practitioners as a means of conserving, reintroducing, and supplementing populations of imperilled freshwater fishes and mussels. We conducted a systematic map to provide an overview of the existing literature on the effectiveness of captive breeding and release programs. A key finding is that there is limited evaluation of the effectiveness of such programs at all three stages (i.e., broodstock collection, rearing/ release methods, and post-release monitoring). We identified clusters of evidence for evaluating supplementation associated with rearing/release methods for fish growth and survival metrics, and the monitoring stage for fish genetic diversity, growth, and survival metrics, primarily focused on salmonids. However, many studies had inadequate experimental designs (i.e., lacked a comparator). Overall, there was a paucity of studies on the effectiveness of captive breeding programs for imperilled freshwater mussels, highlighting the need to make such information broadly available when studies are undertaken. Outputs from this systematic map (i.e., the map database and heatmaps) suggest that the effectiveness of captive breeding and release programs requires further systematic evaluation.
2021 20th IEEE International Conference on Machine Learning and Applications (ICMLA), Dec 1, 2021
Climate change results in altered air and water temperatures. Increases affect physicochemical pr... more Climate change results in altered air and water temperatures. Increases affect physicochemical properties, such as oxygen concentration, and can shift species distribution and survival, with consequences for ecosystem functioning and services. These ecosystem services have integral value for humankind and are forecasted to alter under climate warming. A mechanistic understanding of the drivers and magnitude of expected changes is essential in identifying system resilience and mitigation measures. In this work, we present a selection of state-of-the-art Neural Networks (NN) for the prediction of water temperatures in six streams in Germany. We show that the use of methods that compare observed and predicted values, exemplified with the Root Mean Square Error (RMSE), is not sufficient for their assessment. Hence we introduce additional analysis methods for our models to complement the state-of-the-art metrics. These analyses evaluate the NN's robustness, possible maximal and minimal values, and the impact of single input parameters on the output. We thus contribute to understanding the processes within the NN and help applicants choose architectures and input parameters for reliable water temperature prediction models.
The purpose of this study was to mark endangered sterlet (Acipenser ruthenus) with visible implan... more The purpose of this study was to mark endangered sterlet (Acipenser ruthenus) with visible implant elastomer (VIE) in order to assess mortality, compatibility, retention, persistence and histological reactions resulting from this tagging technique. It was hypothesized there would be only minor effects on the fishes' health, and assumed that acute effects would be more pronounced than long-term effects. On 11 September 2013, 20 specimens were tagged ventrally with visible implant elastomer, 20 received a subcutaneous injection with 0.9% NaCl solution, and another 20 served as untreated control. Mean total length was 28.0 ± 1.8 cm and mean body mass 64.1 ± 12.0 g. The sterlets were kept in four 4,000-L tanks filled with 2,400-L water. Acute effects were monitored for 95 days, where fish were held at temperatures between 2.4°C and 15.2°C, reflecting outdoor conditions. Chronic effects were examined 282 days posttagging through histological sections of the tagging region in five sterlets. During the first 95 days of observation, tag retention was 100%. No signs of incompatibility were detected. Body mass did not significantly differ between VIE-tagged fish and controls. At day 282 post-tagging, however, distinct tissue reactions were visible at the tagging sites of nine fish. Histological examination of five fish revealed a variable degree of infiltration with leukocytes in the areas around the elastomer, which did not necessarily correspond with the externally visible degree of inflammation. After medical treatment, the lesions healed without complications, whereas the retention rate of the VIE tags was 5%. According to the findings, the tag location rather than the tag itself was responsible for the externally visible irritations, indicating that the ventral subcutis of sterlet is not a suitable site, even for small VIE tags in long-term studies. The results of this study also suggest that VIE marking should in general be critically evaluated before application in field studies.
Securing adequate supply of high-quality water is of increasing global importance and relies in l... more Securing adequate supply of high-quality water is of increasing global importance and relies in large part on ecosystem services provided by freshwater biota. Unionid mussels are important keystone species and habitat engineers that shape freshwater ecosystems through water filtration, nutrient cycling and provision of habitats; their rapid global declines result in dramatic losses of ecosystem functions. Maintenance and enhancement of the services they provide depend on the identification of their crucial habitats. Following theoretical assumptions, this study analyses the importance of lake-stream transition zones for unionid mussels, based on data collected in 1984 and 2019 from an undisturbed stream flowing through five consecutive lakes. Mussel distribution matched the distribution of host fish and was strongly influenced by lakes: densities were highest near lake outlets, reaching 290 ind. m-2 (14.7 kg m-2) in 2019, and declined with downstream distance following a negative power function. This pattern was spatially consistent and sustained over time. All six unionid species native to north-central Europe were present, but common species (Anodonta anatina, Unio pictorum, U. tumidus) contributed about 80% of individuals and were responsible for most of the ecosystem services provided by unionid mussels. Estimated 1.9 × 106 mussel individuals inhabiting 3.2 km of stream length filtered a water volume equivalent to the total stream discharge approximately 2.5 times daily. Aggregations of spent shells, up to 17 kg m-2, accumulated downstream of lakes, forming extensive shell and mussel beds, providing habitats and contributing shell hash that improved stream-bed conditions. Globally invasive Dreissena polymorpha was present at low densities and did not spread or increase in abundance, indicating a long-term biotic resistance of the natural native community. Our study underscores the importance of undisturbed lake outlets, longitudinal connectivity of riverine ecosystems, and of common mussel species in maintaining freshwater ecosystem functionality and provision of vital services.
Aquatic Conservation: Marine and Freshwater Ecosystems, 2019
The freshwater pearl mussel (Margaritifera margaritifera) is an endangered species in Europe, pro... more The freshwater pearl mussel (Margaritifera margaritifera) is an endangered species in Europe, protected nationally and internationally, but with a steadily declining range and abundance owing to pressures such as pollution, river engineering, and illegal exploitation. Despite this, no consistent approaches have been developed around Europe for monitoring pearl mussel populations and their habitats. To address this need, experts on pearl mussel ecology from 11 countries met at a series of workshops in order to develop a protocol for monitoring, published under the auspices of the European Committee for Standardization (CEN). This standard is unique, as it is the first CEN standard dedicated to a single species of conservation concern. The standard is aimed at scientists, conservation bodies, and environmental regulators, and can be used for designing national monitoring programmes as well as reporting on the conservation status of pearl mussel populations under the European Habitats ...
Zenodo (CERN European Organization for Nuclear Research), Sep 6, 2018
Despite of structural deficits, highly modified water bodies (HMWB) contain remnant populations o... more Despite of structural deficits, highly modified water bodies (HMWB) contain remnant populations of endangered fish that have high conservation value. Restoration in HMWBs underlies different principles when compared to natural rivers because of impaired river dynamic processes. The objective herein was to assess the contribution of restored habitats of the River Günz, Germany to fish diversity and restoration success of target species. All habitat restoration types, including bank habitats, fast flowing habitats with gravel, structured shallow water zones, nature like fish passes, and a former river course below a spillway were assessed. None of them comprised the full set of 27 fish species or size classes, indicating that the diversity and interlinkage of the restored habitats is most crucial to sustainable fish populations and high biodiversity. When considering the rheophilic target species Chondrostoma nasus and Barbus barbus, only fish passes and the former river course with their high flow current contributed much to their population development, particularly to young specimens <20 cm. The results of this study indicate that measures inside the main channel are unlikely to mitigate deficiencies in HMWBs concerning specialized riverine fish. Instead, interlinkage of a diversity of restoration measures and investment of resources for restoration in HMWBs into nature-like fish passes or former river courses below spillways with dynamically managed discharges seem most useful.
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Papers by Juergen Geist