... i " " L DETECTOR HEAD Y _Jj____l_-3 - P -> Y L - 4 n. Y ... more ... i " " L DETECTOR HEAD Y _Jj____l_-3 - P -> Y L - 4 n. Y E 3 R L - 2 8 - 0 E s 1 ... Two pertinentgraphs typical of - 76 experiments are shown in Figure 9. They represent the profiles seen at the eight detectors (function of time after mixing) for flow rates of 9 mL/min and 6 mL/min. ...
... G. F. REYNOLDS,~ RAYMOND E. DESSY, and H. H. JAFFE ... The absorption peaks at 2980 and 3040 ... more ... G. F. REYNOLDS,~ RAYMOND E. DESSY, and H. H. JAFFE ... The absorption peaks at 2980 and 3040 cm.-l are attributed to CH stretching vibrations, those at 938 and 1010 cm.-' to CH2 andCH out-of-plane deformation vibrations, and that at 1400 cm.-' to a CH2 in-plane ...
A mixed self assembling monolayer (mSAM) chip with attached heparin was developed to analyze hepa... more A mixed self assembling monolayer (mSAM) chip with attached heparin was developed to analyze heparin-protein interactions using a Reichert Inc, SR7000, surface plasmon resonance (SPR) instrument. The heparin was attached via streptavidin-biotin linkage where the streptavidin was covalently coupled to the mSAM and biotinylated heparin bound to it. These chips were then used to quantify the interactions of fibroblast growth factor-2 (FGF-2) with the surface bound heparin. Kinetic rate constants of association and disassociation were calculated. The association data of FGF-2 with heparin was fit to a single compartment, well-mixed model as the data did not exhibit mass transfer limitations. The results suggested that rebinding was prevalent and observed disassociation rates differed significantly in the presence of competing soluble heparin during disassociation. Our results indicate that the Reichert instrument and mSAM chips can be used to analyze heparin-protein interactions but that a careful protocol, outlined in this thesis, should be followed to obtain optimal data.
Discusses various topics related to the interfacing of computers, focusing particularly on method... more Discusses various topics related to the interfacing of computers, focusing particularly on methods of data collection using instruments. Topics include serial digital connections (examining RS 232C and V.24, physical protocol, electrical protocol, and other areas) and parallel digital connections. (JN)
... i " " L DETECTOR HEAD Y _Jj____l_-3 - P -> Y L - 4 n. Y ... more ... i " " L DETECTOR HEAD Y _Jj____l_-3 - P -> Y L - 4 n. Y E 3 R L - 2 8 - 0 E s 1 ... Two pertinentgraphs typical of - 76 experiments are shown in Figure 9. They represent the profiles seen at the eight detectors (function of time after mixing) for flow rates of 9 mL/min and 6 mL/min. ...
... G. F. REYNOLDS,~ RAYMOND E. DESSY, and H. H. JAFFE ... The absorption peaks at 2980 and 3040 ... more ... G. F. REYNOLDS,~ RAYMOND E. DESSY, and H. H. JAFFE ... The absorption peaks at 2980 and 3040 cm.-l are attributed to CH stretching vibrations, those at 938 and 1010 cm.-' to CH2 andCH out-of-plane deformation vibrations, and that at 1400 cm.-' to a CH2 in-plane ...
A mixed self assembling monolayer (mSAM) chip with attached heparin was developed to analyze hepa... more A mixed self assembling monolayer (mSAM) chip with attached heparin was developed to analyze heparin-protein interactions using a Reichert Inc, SR7000, surface plasmon resonance (SPR) instrument. The heparin was attached via streptavidin-biotin linkage where the streptavidin was covalently coupled to the mSAM and biotinylated heparin bound to it. These chips were then used to quantify the interactions of fibroblast growth factor-2 (FGF-2) with the surface bound heparin. Kinetic rate constants of association and disassociation were calculated. The association data of FGF-2 with heparin was fit to a single compartment, well-mixed model as the data did not exhibit mass transfer limitations. The results suggested that rebinding was prevalent and observed disassociation rates differed significantly in the presence of competing soluble heparin during disassociation. Our results indicate that the Reichert instrument and mSAM chips can be used to analyze heparin-protein interactions but that a careful protocol, outlined in this thesis, should be followed to obtain optimal data.
Discusses various topics related to the interfacing of computers, focusing particularly on method... more Discusses various topics related to the interfacing of computers, focusing particularly on methods of data collection using instruments. Topics include serial digital connections (examining RS 232C and V.24, physical protocol, electrical protocol, and other areas) and parallel digital connections. (JN)
Uploads
Papers by Raymond Dessy