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(PDF) Phytophthora Species in Serbia
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Phytophthora Species in Serbia

Since there is a great economic and ecological threat posed by Phytophthora species, a study has been performed during the years 2009-2012, aiming at determining the presence and diversity of Phytophthora species in both natural ecosystems and amenity trees in Serbia. Sampled trees showed symptoms typical of Phytophthora infections, such as presence of collar rots or stem cankers with dark exudates, chlorosis and wilting of leaves, increased crown transparency, dieback, dying of shoots, branches or parts of the crown, root lesions, and decay and loss of fine roots.

PHYTOPHTHORA SPECIES IN SERBIA Ivan Milenkovic¹, Nenad Keca¹, Justyna Nowakowska², Katarzyna Sikora², Malgorzata Borys², Tomasz Oszako², Thomas Jung³,4 ¹University of Belgrade, Faculty of Forestry, 1, Kneza Viseslava street, 11030, Belgrade, Serbia ²Forest Research Institute – IBL, ul. Braci Lesnej 3, Sekocin Stary, 0509 – Raszyn, Poland ³Phytophthora Research and Consultancy, Thomastrasse 75., Brannenburg, Germany 4 IBB/CGB Plant and Animal Genomic Group, Laboratório de Biotecnologia Molecular e Fitopatologia, Universidade do Algarve, 8005-139 Faro, Portugal. ivan.milenkovic@sfb.bg.ac.rs Since there is a great economic and ecological threat posed by Phytophthora species, a study has been performed during the years 2009-2012, aiming at determining the presence and diversity of Phytophthora species in both natural ecosystems and amenity trees in Serbia. Sampled trees showed symptoms typical of Phytophthora infections, such as presence of collar rots or stem cankers with dark exudates, chlorosis and wilting of leaves, increased crown transparency, dieback, dying of shoots, branches or parts of the crown, root lesions, and decay and loss of fine roots. Sampling and isolation methods were according to Jung (2009) and Jung et al. (1996). Tissue samples were taken from necrotic parts and plated directly onto selective agar medium (V8A-PARPNH). Soil containing fine roots was sampled in the form of soil monoliths, measuring ~ 25x25x25 cm, and isolation tests were performed using oak, beech and cherry laurel leaves as baits. Both symptomatic and healthy trees were sampled. In total 167 samples were taken from 26 different host species including Quercus robur, Q. petraea, Q. cerris, Fagus sylvatica, Fraxinus angustifolia, Acer pseudoplatanus, A. platanoides, A. heldreichii, Populus spp., Juglans regia and Betula pendula, in both forest ecosystems and amenity tree stands. Phytophthora species were isolated from about 68% of samples. Also, many isolates of Pythium spp. were obtained. After a detailed morphological and molecular identification of all isolates, nine different Phytophthora species have been confirmed, i.e. P. europaea, P. cambivora, P. citricola, P. cactorum, P. plurivora, P. polonica, P. quercina, P. taxon ´Pg chlamydo‘, and P. lacustris (previously known as P. taxon ‘Salixsoil’), and some of them were recorded for the first time in different ecosystems in Serbia. Acknowledgements We are grateful to COST FP0801-Short Term Scientific Missions and to project TR 37008, Ministry of Education and Science, Republic of Serbia for material support during these studies. Literature: Jung, T., 2009: Beech decline in Central Europe driven by the interactions between Phytophthora infections and climatic extremes. For. Pathol. 38, 73-94. Jung, T.; Blaschke, H.; Neumann, P., 1996: Isolation, identification and pathogenicity of Phytophthora species from declining oak stands. Eur. J. Forest Pathol. 26, 253-272. View publication stats








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