Recombinant E. coli proteins for research use.

We are a Biotech firm working mainly in the production of custom made recombinants. Our proteins are used all over the world, in University and Pharmaceutical Laboratories.

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The EcoGene database contains updated information about the E. coli K-12 genome and proteome sequences, including extensive gene bibliographies. A major EcoGene focus has been the re-evaluation of translation start sites. The re-annotated start sites of EcoGene have now been incorporated into the E. coli MG1655 genome sequence GenBank record U00096.2. Some revisions are based on an EcoGene literature survey, called the Verified Set, of N-terminally sequenced E. coli proteins.

The OU E.coli Gene Expression Database allows researchers to query gene expression data for E. coli, with tools to examine how specific genes are expressed under various environmental or experimental conditions. It provides access to expression levels, gene annotations, and details on regulatory pathways that affect gene activity. This can aid in understanding the molecular mechanisms of bacterial function and disease.

Title	Tryptophan metabolism response in E. coli
Organism(s)	Escherichia coli
Summary	DNA microarray analysis of gene expression in response to physiological and genetic changes that affect tryptophan metabolism in Escherichia coli. We investigated the global changes in mRNA abundance in Escherichia coli elicited by various perturbations of tryptophan metabolism. To do so we printed DNA microarrays containing 95% of all annotated E. coli ORFs. We determined the expression profile that is predominantly dictated by the activity of the tryptophan repressor. Only three operons, trp, mtr, and aroH, exhibited appreciable expression changes consistent with this profile. The quantitative changes we observed in mRNA levels for the five genes of the trp operon were consistent within a factor of 2, with expectations based on established Trp protein levels. Several operons known to be regulated by the TyrR protein, aroF-tyrA, aroL, aroP, and aroG, were down-regulated on addition of tryptophan. TyrR can be activated by any one of the three aromatic amino acids. Only one operon, tnaAB, was significantly activated by the presence of tryptophan in the medium. We uncovered a plethora of likely indirect effects of changes in tryptophan metabolism on intracellular mRNA pools, most prominent of which was the sensitivity of arginine biosynthetic operons to tryptophan starvation. This study is detailed in Khodursky AB et al.(2000) Proc Natl Acad Sci U S A 97:12170-5

Organism	Highlights
Escherichia coli, Strain K-12 MG1655 (EcoCyc)	Literature curation of complete genome Information from 17,000 publications Transcriptional regulatory network Protein complexes Enzyme and transporter functions Gene Ontology assignments
Escherichia coli, Strain K-12 W3110	Laboratory strain
Escherichia coli, Strain SMS-3-5	Environmental isolate
Escherichia coli, Strain CFT073	Uropathogenic strain (UPEC)
Escherichia coli, Strain 536	Uropathogenic strain (UPEC)
Escherichia coli, Strain UTI89	Uropathogenic strain (UPEC)
Escherichia coli, Strain EDL933, serotype O157:H7	Enterohaemorrhagic strain (EHEC)
Escherichia coli, Strain O157:H7 VT2-Sakai	Enterohaemorrhagic strain (EHEC)
Shigella flexneri, Strain 2457T, serotype 2a	Enteropathogenic strain, shigellosis
Shigella flexneri, Strain 301, serotype 2a	Enteropathogenic strain, shigellosis
Shigella flexneri, Strain 8401, serotype 5	Enteropathogenic strain, dysentery
Shigella boydii, Strain Sb227	Enteropathogenic strain, dysentery
Shigella dysenteriae, Strain Sd197	Enteropathogenic strain, dysentery
Shigella sonnei, Strain Ss046	Enteropathogenic strain, dysentery