Skip to main content
Springer Nature Link
Log in

Halotolerant Aerobic Heterotrophic Bacteria from the Great Salt Plains of Oklahoma

  • Microbial Observatories
  • Published:
Microbial Ecology Aims and scope Submit manuscript

Abstract

The Salt Plains National Wildlife Refuge (SPNWR) near Cherokee, Oklahoma, contains a barren salt flat where Permian brine rises to the surface and evaporates under dry conditions to leave a crust of white salt. Rainfall events dissolve the salt crust and create ephemeral streams and ponds. The rapidly changing salinity and high surface temperatures, salinity, and UV exposure make this an extreme environment. The Salt Plains Microbial Observatory (SPMO) examined the soil microbial community of this habitat using classic enrichment and isolation techniques and phylogenetic rDNA studies. Rich growth media have been emphasized that differ in total salt concentration and composition. Aerobic heterotrophic enrichments were performed under a variety of conditions. Heterotrophic enrichments and dilution plates have generated 105 bacterial isolates, representing 46 phylotypes. The bacterial isolates have been characterized phenotypically and subjected to rDNA sequencing and phylogenetic analyses. Fast-growing isolates obtained from enrichments with 10% salt are predominantly from the gamma subgroup of the Proteobacteria and from the low GC Gram-positive cluster. Several different areas on the salt flats have yielded a variety of isolates from the Gram-negative genera Halomonas, Idiomarina, Salinivibrio, and Bacteroidetes. Gram-positive bacteria are well represented in the culture collection including members of the Bacillus, Salibacillus, Oceanobacillus, and Halobacillus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11

Similar content being viewed by others

References

  1. AM Al-Tai J-S Ruan (1994) ArticleTitleNocardiopsis halophila sp. nov., a new halophilic actinomycete isolated from soil. Int J System Bacteriol 44 474–478

    Google Scholar 

  2. SF Altschul W Gish W Miller EW Myers DJ Lipman (1990) ArticleTitleBasic local alignment search tool. J Mol Biol 215 403–410 Occurrence Handle10.1006/jmbi.1990.9999 Occurrence Handle1:CAS:528:DyaK3MXitVGmsA%3D%3D Occurrence Handle2231712

    Article  CAS  PubMed  Google Scholar 

  3. J Antón R Rosselló-Mora F Rodríguez-Valera R Amann (2000) ArticleTitleExtremely halophilic Bacteria in crystallizer ponds from solar salterns. Appl Environ Microbiol 66 3052–3057 Occurrence Handle10.1128/AEM.66.7.3052-3057.2000 Occurrence Handle10877805

    Article  PubMed  Google Scholar 

  4. DR Arahal MT García C Vargas D Cánovas JJ Nieto A Ventosa (2001) ArticleTitleChromohalobacter salexigens sp. nov., a moderately halophilic species that includes Halomonas elongata DSM 3043 and ATCC 33174. Int J System Evol Microbiol 51 1457–1462 Occurrence Handle1:CAS:528:DC%2BD3MXmtVSlsr8%3D

    CAS  Google Scholar 

  5. DR Arahal MC Márquez BE Volcani KH Schleifer A Ventosa (1999) ArticleTitleBacillus marismortui sp. nov., a new moderately halophilic species from the Dead Sea. Int J System Evol Microbiol 49 521–530

    Google Scholar 

  6. DR Arahal MC Márquez BE Volcani KH Schleifer A Ventosa (2000) ArticleTitleReclassification of Bacillus marismortui as Salibacillus marismortui comb. nov. Int J System Evol Microbiol 50 1501–1503

    Google Scholar 

  7. C Ash JAE Farrow S Wallbanks MD Collins (1991) ArticleTitlePhylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences. Lett Appl Microbiol 13 202–206 Occurrence Handle1:CAS:528:DyaK38XhsVOqsL8%3D

    CAS  Google Scholar 

  8. RM Atlas (1993) Handbook of Microbiological Media CRC Press Boca Raton, FL 64

    Google Scholar 

  9. B Austin (1989) ArticleTitleA review: novel pharmaceutical compounds from marine bacteria. J Appl Bacteriol 67 461–468 Occurrence Handle1:CAS:528:DyaK3cXkt1aqtg%3D%3D Occurrence Handle2687222

    CAS  PubMed  Google Scholar 

  10. JR Battista (1997) ArticleTitleAgainst all odds: the survival strategies of Deinococcus radiodurans. Ann Rev Microbiol 51 203–224 Occurrence Handle1:CAS:528:DyaK2sXms1Shsbg%3D

    CAS  Google Scholar 

  11. S Baumgarte ERB Moore BJ Tindall (2001) ArticleTitleRe-examining the 16S rDNA sequence of Halomonas salina. Int J System Evol Microbiol 51 51–53 Occurrence Handle1:CAS:528:DC%2BD3MXhtlaksrw%3D

    CAS  Google Scholar 

  12. S Benlloch AJ Martinez-Murcia F Rodriquez-Valera (1995) ArticleTitleSequencing of bacterial and archaeal 16S rRNA genes directly amplified from a hypersaline environment. Syst Appl Microbiol 18 574–581

    Google Scholar 

  13. N Bozal MJ Monies E Tudela J Guinea (2003) ArticleTitleCharacterization of several Psychrobacter strains isolated from Antarctic environments and description of Psychrobacter luti sp. nov. and Psychrobacter fozii sp. nov. Int J Syst Evol 53 1093–1100 Occurrence Handle10.1099/ijs.0.02457-0 Occurrence Handle1:CAS:528:DC%2BD3sXmsF2it74%3D

    Article  CAS  Google Scholar 

  14. I Brettar R Christen MG Hofle (2003) ArticleTitleIdiomarina baltica sp. nov., a marine bacterium with a high optimum growth temperature isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 53 407–413 Occurrence Handle10.1099/ijs.0.02399-0 Occurrence Handle1:CAS:528:DC%2BD3sXjtVKkur4%3D Occurrence Handle12710605

    Article  CAS  PubMed  Google Scholar 

  15. S Castanier J-P Perthuisot J-M Rouchy A Maurin O Guelorget (1992) ArticleTitleHalite ooids in Lake Asal, Djibouti: biocrystalline build-ups. Geobios 25 811–821

    Google Scholar 

  16. Caton, TM, Witte, LR, Buchheim, JA, Buchheim, MA, Schneegurt, MA (2003) Phenetic and phylogenetic characterization of the prokaryotic assemblage at the Great Salt Plains of Oklahoma. Abs 102nd Gen Mtg Am Soc Microbiol N-321:455–456

  17. Chai, LH, Cui, XL, Wang, T, Xu, LH, Jiang, CL (2002) The microbial diversity of Salt Lake Keke in Western China. GenBank no. AY121439

  18. JR Cole B Chai TL Marsh RJ Farris Q Wang SA Kulam S Chandra DM McGarrell TM Schmidt GM Garrity JM Tiedje (2003) ArticleTitleThe Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31 442–443 Occurrence Handle10.1093/nar/gkg039 Occurrence Handle1:CAS:528:DC%2BD3sXhvFSls7o%3D Occurrence Handle12520046

    Article  CAS  PubMed  Google Scholar 

  19. LN Csonka (1989) ArticleTitlePhysiological and genetic responses of bacteria to osmotic stress. Microbiol Rev 53 121–147 Occurrence Handle1:CAS:528:DyaL1MXhs12rt7w%3D Occurrence Handle2651863

    CAS  PubMed  Google Scholar 

  20. Dobson, SJ, Franzmann, PD (1995). GenBank no. L42618

  21. SJ Dobson PD Franzmann (1996) ArticleTitleUnification of the genera Deleya (Baumann et al. 1983), Halomonas (Vreeland et al. 1980), and Halovibrio (Fendic 1988) and the species Paracoccus halodenitrificans (Robinson and Gibbons 1952) into a single genus, Halomonas, and placement of the genus Zymobacter in the family Halomonadaceae. Int J Syst Bacteriol 46 550–558 Occurrence Handle1:CAS:528:DyaK28XivFajsbc%3D

    CAS  Google Scholar 

  22. SP Donachie S Hou TS Gregory A Malahoff M Alam (2003) ArticleTitleIdiomarina loihiensis sp. nov., a halophilic gamma-Proteobacterium from the Lo’ihi submarine volcano, Hawai’i. Int J Syst Evol Microbiol 53 1873–1879 Occurrence Handle10.1099/ijs.0.02701-0 Occurrence Handle1:CAS:528:DC%2BD2cXhtF2hsA%3D%3D Occurrence Handle14657116

    Article  CAS  PubMed  Google Scholar 

  23. U Edwards H Rogall H Blöcker M Emde EC Böttger (1989) ArticleTitleIsolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res 17 7843–7853 Occurrence Handle1:CAS:528:DyaK3cXjtFKksg%3D%3D Occurrence Handle2798131

    CAS  PubMed  Google Scholar 

  24. JA Farrow C Ash S Wallbanks MD Collins (1992) ArticleTitlePhylogenetic analysis of the genera Planococcus, Marinococcus and Sporosarcina and their relationship to members of the genus Bacillus. FEMS Microbiol Lett 72 167–172 Occurrence Handle10.1016/0378-1097(92)90523-Q Occurrence Handle1:STN:280:By2A2s7ntlI%3D Occurrence Handle1505740

    Article  CAS  PubMed  Google Scholar 

  25. C Fendrich (1988) ArticleTitleHalovibrio variabilis gen. nov. sp. nov., Pseudomonas halophila sp. nov. and a new halophilic aerobic coccoid eubacterium from Great Salt Lake, Utah, USA. Syst Appl Microbiol 11 36–43 Occurrence Handle1:CAS:528:DyaL1MXhtFWmt7w%3D

    CAS  Google Scholar 

  26. X Fu FJ Schmitz RS Tanner (1995) ArticleTitleChemical constituents of halophilic facultatively anaerobic bacteria. J Nat Prod 58 1950–1954

    Google Scholar 

  27. EA Galinski BJ Tindall (1992) Biotechnological prospects for halophiles and halotolerant micro-organisms. RA Herbert RJ Sharp (Eds) Molecular Biology and Biotechnology of Extremophiles Thomson Press New Delhi 76–114

    Google Scholar 

  28. MJ Garabito MC Márquez A Ventosa (1998) ArticleTitleHalotolerant Bacillus diversity in hypersaline environments. Can J Microbiol 44 95–102 Occurrence Handle10.1139/cjm-44-2-95 Occurrence Handle1:CAS:528:DyaK1cXitlektr4%3D

    Article  CAS  Google Scholar 

  29. K Goto T Omura Y Hara Y Sadaie (2000) ArticleTitleApplication of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus. J Gen Appl Microbiol 46 1–8

    Google Scholar 

  30. MV Hao M Kocur K Komagata (1984) ArticleTitleMarinococcus gen. nov., a new genus for motile cocci with meso-diaminopimelic acid in the cell wall, and Marinococcus albus sp. nov. and Marinococcus halophilus (Novitskyand Kushner) comb. nov. J Gen Appl Microbiol 30 449–459

    Google Scholar 

  31. Y Henis J Eren (1963) ArticleTitlePreliminary studies on the microflora of a highly saline soil. Can J Microbiol 9 902–904

    Google Scholar 

  32. M Heyndrickx L Lebbe K Kersters P De Vos C Forsyth NA Logan (1998) ArticleTitleVirgibacillus: a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus. Int J Syst Bacteriol 48 99–106

    Google Scholar 

  33. J Heyrman NA Logan H-J Busse A Balcaen L Lebbe M Rodriguez-Diaz J Swings P De Vos (2003) ArticleTitleVirgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus Salibacillus to Virgibacillus, as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus. Int J Syst Evol Microbiol 53 501–511 Occurrence Handle10.1099/ijs.0.02371-0 Occurrence Handle1:CAS:528:DC%2BD3sXjtVKku7w%3D Occurrence Handle12710619

    Article  CAS  PubMed  Google Scholar 

  34. J Heyrman J Swings (2001) ArticleTitle16S rDNA sequence analysis of bacterial isolates from biodeteriorated mural paintings in the Servilia tomb (Necropolis of Carmona, Seville, Spain). Syst Appl Microbiol 24 417–422 Occurrence Handle1:CAS:528:DC%2BD38XhsFSiur8%3D Occurrence Handle11822679

    CAS  PubMed  Google Scholar 

  35. C-Y Huang J-L Garcia BKC Patel J-L Cayol L Baresi RA Mah (2000) ArticleTitleSalinivibrio costicola subsp. vallismortis subsp. nov., a halotolerant facultative anaerobe from Death Valley, and emended description of Salinivibrio costicola. Int J Syst Evol Microbiol 50 615–622 Occurrence Handle1:CAS:528:DC%2BD3cXivFaisro%3D Occurrence Handle10758867

    CAS  PubMed  Google Scholar 

  36. SB Humayoun N Bano JT Hollibaugh (2003) ArticleTitleDepth distribution of microbial diversity in Mono Lake, a meromictic soda lake in California. Appl Environ Microbiol 69 1030–1042 Occurrence Handle10.1128/AEM.69.2.1030-1042.2003 Occurrence Handle1:CAS:528:DC%2BD3sXhtF2itr4%3D Occurrence Handle12571026

    Article  CAS  PubMed  Google Scholar 

  37. EP Ivanova LA Romanenko J Chun MH Matte GR Matte VV Mikahailov VI Svetashev A Huq T Maugel RR Colwell (2000) ArticleTitleIdiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including descriptions of two species, Idiomarina abyssalis sp. nov. and Idiomarina zobelli sp. nov. Int J Syst Evol Microbiol 50 901–907 Occurrence Handle1:CAS:528:DC%2BD3cXivFaisb0%3D Occurrence Handle10758902

    CAS  PubMed  Google Scholar 

  38. B Javor (1989) Hypersaline Environments: Microbiology and Biogeochemistry Springer-Verlag New York 77–100

    Google Scholar 

  39. KS Johnson (1980) Guidebook for Geologic Field Trips in Oklahoma. Book II: Northwest Oklahoma University of Oklahoma Norman, OK, 13–15

    Google Scholar 

  40. BE Jones WD Grant AW Duckworth GG Owenson (1998) ArticleTitleMicrobial diversity of soda lakes. Extremophiles 2 191–200 Occurrence Handle10.1007/s007920050060 Occurrence Handle1:CAS:528:DyaK1cXmtVOqt70%3D Occurrence Handle9783165

    Article  CAS  PubMed  Google Scholar 

  41. E Juni GA Heym (1986) ArticleTitlePsychrobacter immobilis gen. nov., sp. nov.: genospecies composed of Gram-negative, aerobic, oxidase-positive coccobacilli. Int J Syst Bacteriol 36 388–391

    Google Scholar 

  42. M Kamekura Y Seno (1990) ArticleTitleA halophilic extracellular protease from a halophilic archaebacterium strain. Biochem Cell Biol 68 352–359 Occurrence Handle1:CAS:528:DyaK3cXhslCmu7k%3D Occurrence Handle2112401

    CAS  PubMed  Google Scholar 

  43. J Kushnir (1981) ArticleTitleFormation and early diagenesis of varved evaporite sediments in a coastal hypersaline pool. J Sed Petrol 51 1193–1203

    Google Scholar 

  44. CD Litchfield (1998) ArticleTitleSurvival strategies for microorganisms in hypersaline environments and their relevance to life on early Mars. Meteor Planet Sci 33 813–819 Occurrence Handle1:CAS:528:DyaK1cXls12mtbs%3D

    CAS  Google Scholar 

  45. CD Litchfield PM Gillevet (2002) ArticleTitleMicrobial diversity and complexity in hypersaline environments: a preliminary assessment. J Ind Microbiol Biotechnol 28 48–55

    Google Scholar 

  46. CD Litchfield A Irby RH Vreeland (1998) The microbial ecology of solar salt plants. A Oren (Eds) Microbiology and Biogeochemistry of Hypersaline Environments CRC Press Boca Raton, FL 39–52

    Google Scholar 

  47. J Lu Y Nogi H Takami (2001) ArticleTitleOceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge. FEMS Microbiol Lett 205 291–297 Occurrence Handle10.1016/S0378-1097(01)00493-1 Occurrence Handle1:CAS:528:DC%2BD3MXpt1als7Y%3D Occurrence Handle11750818

    Article  CAS  PubMed  Google Scholar 

  48. Maas, EW, Kodama, M, Thiele, JH, Bennasar, A, Brooks, HJL, Moore, ERB (1997) Phylogenetic characterization of paralytic shellfish toxin (PST) producing bacteria isolated from marine algal cultures. GenBank no. AJ000648

  49. Maddison, DR, Maddison, WP (2000) MacClade 4: Analysis of Phylogeny and Character Evolution. Sinauer Associates, Sunderland, MA

    Google Scholar 

  50. PL Manachini MG Fortina L Levati C Parini (1998) ArticleTitleContribution to phenotypic and genotypic characterization of Bacillus licheniformis and description of new genomovars. Sys Appl Microbiol 21 520–529 Occurrence Handle1:CAS:528:DyaK1MXhtV2mur0%3D

    CAS  Google Scholar 

  51. JA Mata J Martínez-Cánovas E Quesada V Béjar (2002) ArticleTitleA Halomonas detailed phenotypic characterization of the type strains of species. Syst Appl Microbiol 25 360–375 Occurrence Handle1:CAS:528:DC%2BD38XpsV2ru78%3D Occurrence Handle12421074

    CAS  PubMed  Google Scholar 

  52. E Mellado ERB Moore JJ Nieto A Ventosa (1995) ArticleTitlePhylogenetic inferences and taxonomic consequences of 16S ribosomal DNA sequence comparison of Chromohalobacter marismortui, Volcaniella eurihalina, and Deleya salina and reclassification of V. eurihalina as Halomonas eurihalina comb. nov. Int J Syst Bacteriol 45 712–716 Occurrence Handle1:CAS:528:DyaK28XivFGj Occurrence Handle7547290

    CAS  PubMed  Google Scholar 

  53. E Mellado ERB Moore JJ Nieto A Ventosa (1996) ArticleTitleAnalysis of 16S rRNA gene sequences of Vibrio costicola strains: description of Salinivibrio costicola gen. nov., comb. nov. Int J Syst Bacteriol 46 817–821 Occurrence Handle1:CAS:528:DyaK28XkvV2nsLY%3D Occurrence Handle8782695

    CAS  PubMed  Google Scholar 

  54. RV Miller (1992) recA. S Luria (Eds) Encyclopedia of Microbiology Academic Press San Diego 509–517

    Google Scholar 

  55. RV Miller (2000) recA: the gene and its protein product. S Luria (Eds) Encyclopedia of Microbiology Academic Press San Diego 43–54

    Google Scholar 

  56. RV Miller TA Kokjohn (1990) ArticleTitleGeneral microbiology of recA: environmental and evolutionary significance. Ann Rev Microbiol 44 365–394 Occurrence Handle1:CAS:528:DyaK3MXnvVagug%3D%3D

    CAS  Google Scholar 

  57. P Nielsen FA Rainey H Outtrup FG Priest D Fritze (1994) ArticleTitleComparative 16S rDNA sequence analysis of some alkaliphilic bacilli and the establishment of a sixth rRNA group within the genus Bacillus. FEMS Microbiol Lett 117 61–66 Occurrence Handle10.1016/0378-1097(94)90171-6 Occurrence Handle1:CAS:528:DyaK2cXltVeluro%3D

    Article  CAS  Google Scholar 

  58. T Ochsenreiter F Pfeifer C Schleper (2002) ArticleTitleDiversity of Archaea in hypersaline environments characterized by molecular-phylogenetic and cultivation studies. Extremophiles 6 267–274 Occurrence Handle10.1007/s00792-001-0253-4 Occurrence Handle1:CAS:528:DC%2BD38XnsVSnsLo%3D Occurrence Handle12215811

    Article  CAS  PubMed  Google Scholar 

  59. A Oren (1990) ArticleTitleEstimation of the contribution of halobacteria to the bacterial biomass and activity in solar salterns by the use of bile salts. FEMS Microb Ecol 73 41–48 Occurrence Handle10.1016/0378-1097(90)90722-3 Occurrence Handle1:CAS:528:DyaK3cXht1Cltbo%3D

    Article  CAS  Google Scholar 

  60. A Oren (1999) ArticleTitleBioenergetic aspects of halophilism. Microbiol Molec Biol Rev 63 334–348 Occurrence Handle1:STN:280:DyaK1M3os1eksw%3D%3D

    CAS  Google Scholar 

  61. MM Palmisano LK Nakamura KE Duncan CA Istock FM Cohan (2001) ArticleTitleBacillus sonorensis sp. nov., a close relative of Bacillus licheniformis, isolated from soil in the Sonoran Desert, Arizona. Int J System Evol Microbiol 51 1671–1679 Occurrence Handle1:CAS:528:DC%2BD3MXns1yqsL8%3D

    CAS  Google Scholar 

  62. B Prado A Del Moral E Quesada R Ríos M Monteoliva-Sanchez V Campos A Ramos-Cormenzana (1991) ArticleTitleNumerical taxonomy of moderately halophilic Gram-negative rods isolated from the Salar de Atacama, Chile. Syst Appl Microbiol 14 275–281

    Google Scholar 

  63. E Quesada V Bejar MJ Valderrama A Ventosa A Ramos-Cormenzana (1985) ArticleTitleIsolation and characterization of moderately halophilic nonmotile rods from different saline habitats. Microbiología 1 89–96 Occurrence Handle1:STN:280:By%2BD38jlt1U%3D

    CAS  Google Scholar 

  64. E Quesada A Ventosa F Rodriguez-Valera L Megias A Ramos-Cormenzana (1983) ArticleTitleNumerical taxonomy of moderately halophilic Gram-negative bacteria from hypersaline soils. J Gen Microbiol 129 2649–2657

    Google Scholar 

  65. Reed, JE (1982) Preliminary projections of the effects of chloride-control structures on the quaternary aquifer at the Great Salt Plains, Oklahoma, US Geological Survey, Water-Resources Investigations 80–120, Oklahoma City, Oklahoma

  66. MA Rivadeneyra G Delgado M Soriano A Ramos-Cormenzana R Delgado (1983) ArticleTitleBiomineralization of carbonates by Marinococcus albus and Marinococcus halophilus isolated from the Salar de Atacama (Chile). Curr Microbiol 39 53–57

    Google Scholar 

  67. F Rodriguez-Valera (1992) ArticleTitleBiotechnological potential of halobacteria. Biochem Soc Symp 58 135–147 Occurrence Handle1:STN:280:ByyD2srivVM%3D Occurrence Handle1445403

    CAS  PubMed  Google Scholar 

  68. F Rodriguez-Valera F Riuz-Berraquero A Ramos-Cormenzana (1980) ArticleTitleIsolation of extremely halophilic bacteria able to grow in defined organic media with single carbon sources. J Gen Microbiol 119 535–538

    Google Scholar 

  69. F Rodriguez-Valera A Ventosa G Juez JF Imhoff (1985) ArticleTitleVariation of environmental features and microbial populations with salt concentrations in a multi-pond saltern. Microb Ecol 11 107–111

    Google Scholar 

  70. LJ Rothschild (1999) Microbes and radiation. J Seckbach (Eds) Enigmatic Microorganisms and Life in Extreme Environments Kluwer Academic Publishers Dordrecht 551–562

    Google Scholar 

  71. LJ Rothschild LJ Giver MR White RL Mancinelli (1994) ArticleTitleMetabolic activity of microorganisms in evaporites. J Phycol 30 431–438 Occurrence Handle10.1111/j.0022-3646.1994.00431.x Occurrence Handle1:STN:280:DC%2BD3MnlvVeqtg%3D%3D Occurrence Handle11539827

    Article  CAS  PubMed  Google Scholar 

  72. H Schlesner PA Lawson MD Collins N Weiss U Wehmeyer H Volker M Thomm (2001) ArticleTitleFilobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with Orn-D-Glu-type peptidoglycan. Int J Syst Evol Microbiol 51 425–431 Occurrence Handle1:CAS:528:DC%2BD3MXivVOrtbo%3D Occurrence Handle11324591

    CAS  PubMed  Google Scholar 

  73. Schneegurt, MA, Caton, TM, Nguyen, HD, Potter, A, Henley, WJ (2002) Initial characterization of the microbial community of the Great Salt Plains of Oklahoma. Abs 102nd Gen Mtg Am Soc Microbiol N-141:329

  74. S Spring W Ludwig MC Marquez A Ventosa K-H Schleifer (1996) ArticleTitleHalobacillus gen. nov., with descriptions of Halobacillus litoralis sp. nov. and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov. Int J Syst Bacteriol 46 492–496

    Google Scholar 

  75. Suemori, A, Nakajima, K (1998) Isolation and characterization of alkaliphilic Bacillus sp. assimilating inulin: Bacillus alcaliinulinus sp. nov. GenBank no. AB018595

    Google Scholar 

  76. Swofford, DL (2002) PAUP*: Phylogenetic Analysis Using Parsimony, Version 4.0b10. Sinauer Associates, Sunderland, MA

  77. H Takami TA Krulwich (2000) ArticleTitleReidentification of facultatively alkaliphilic Bacillus firmus OF4 as Bacillus pseudofirmus OF4. Extremophiles 4 19–22 Occurrence Handle1:STN:280:DC%2BD3c3gtlyktQ%3D%3D Occurrence Handle10741833

    CAS  PubMed  Google Scholar 

  78. H Takami Y Takaki I Uchiyama (2002) ArticleTitleGenome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments. Nucleic Acids Res 30 3927–3935 Occurrence Handle10.1093/nar/gkf526 Occurrence Handle1:CAS:528:DC%2BD38Xnt1Gmsr0%3D Occurrence Handle12235376

    Article  CAS  PubMed  Google Scholar 

  79. Tanner, RS, Poynter, NL, Mandelco, L, Woese, CR (1994) Vibrio aspartigenicus, sp. nov., a halophilic, facultatively anaerobic eubacterium that produces aspartic acid. GenBank Taxon ID 36872

  80. V Torsvik J Goksøyr FL Daae (1990) ArticleTitleHigh diversity of DNA of soil bacteria. Appl Environ Microbiol 56 782–787

    Google Scholar 

  81. MJ Valderamma E Quesada V Bejar A Ventosa MC Gutierrez F Ruiz-Berraquero A Ramos-Cormenzanna (1993) ArticleTitleDeleya salina sp. nov., a moderately halophilic Gram-negative bacterium. Int J Syst Bacteriol 41 377–384

    Google Scholar 

  82. K Venkateswaran N Hattori MT La Duc R Kern (2003) ArticleTitleATP as a biomarker of viable microorganisms in clean-room facilities. J Microbiol Methods 52 367–377 Occurrence Handle10.1016/S0167-7012(02)00192-6 Occurrence Handle1:CAS:528:DC%2BD3sXjtFyquw%3D%3D Occurrence Handle12531506

    Article  CAS  PubMed  Google Scholar 

  83. A Ventosa E Quesada F Rodriguez-Valera F Ruiz-Berraquero A Ramos-Cormenzana (1982) ArticleTitleNumerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128 1955–1968

    Google Scholar 

  84. RH Vreeland EL Martin (1980) ArticleTitleGrowth characteristics, effects of temperature, and ion specificity of the halotolerant bacterium Halomonas elongata. Can J Microbiol 26 746–752 Occurrence Handle1:CAS:528:DyaL3cXltFSgtbw%3D

    CAS  Google Scholar 

  85. M Waino BJ Tindall K Ingvorsen (2000) ArticleTitleHalorhabdus utahensis gen. nov., sp. nov., an aerobic, extremely halophilic member of the Archaea from Great Salt Lake, Utah. Int J Syst Evol Microbiol 50 183–190 Occurrence Handle10826803

    PubMed  Google Scholar 

  86. JH Yoon KH Kang YH Park (2003) ArticleTitleHalobacillus salinus sp. nov., isolated from a salt lake on the coast of the East Sea in Korea. Int J Syst Evol Microbiol 53 687–693 Occurrence Handle10.1099/ijs.0.02421-0 Occurrence Handle1:CAS:528:DC%2BD3sXkvFOjurY%3D Occurrence Handle12807188

    Article  CAS  PubMed  Google Scholar 

  87. JH Yoon IG Kim KH Kang TK Oh YH Park (2003) ArticleTitleBacillus marisflavi sp. nov. and Bacillus aquaemaris sp. nov. isolated from seawater of a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 53 1297–1303 Occurrence Handle10.1099/ijs.0.02365-0 Occurrence Handle1:CAS:528:DC%2BD3sXotlymu7s%3D Occurrence Handle13130010

    Article  CAS  PubMed  Google Scholar 

  88. Yoon, JH, Kim, IK, Kang, KH, Park, YH (2002) Bacillus hwajinpoensis sp. nov. and Bacillus baekryungensis sp. nov., new members of Bacillus rRNA group 6 isolated from seawater of the East Sea and the Yellow Sea in Korea. GenBank no. AF541965

  89. JH Yoon KC Lee YH Kho KH Kang CJ Kim YH Park (2002b) ArticleTitleHalomonas alimentaria sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 52 123–130 Occurrence Handle1:CAS:528:DC%2BD38XhsVWmtLg%3D

    CAS  Google Scholar 

  90. I Yumoto S Yamaga Y Sogabe Y Nodasaka H Matsuyama K Nakajima A Suemori (2003) ArticleTitleBacillus krulwichiae sp. nov., a halotolerant obligate alkaliphile that utilizes benzoate and m-hydroxybenzoate. Int J Syst Evol Microbiol 53 1531–1536 Occurrence Handle10.1099/ijs.0.02596-0 Occurrence Handle1:CAS:528:DC%2BD3sXotlyltL8%3D Occurrence Handle13130043

    Article  CAS  PubMed  Google Scholar 

  91. Zhang, W, Ma, Y, Mao, W (2000) Unidentified Hailaer soda lake bacterium T10. GenBank no. AF275707

Download references

Acknowledgments

The authors appreciate the technical assistance of Karen Draeger, Darren Francisco, Jennifer Hanna, Paige Hatcher, Brandon Litzner, Bobbie Pettriess, Noah Schneegurt, and Adrianne Wedel. We are grateful for the support provided by the other members of the SPMO, Drs. William Henley, Andrea Kirkwood, and Robert Miller, and their staff and students. Weather data were provided free of charge from the Oklahoma Mesonet, managed by the University of Oklahoma and Oklahoma State University. Primary support for this work was provided by grants from the Microbial Observatories program of the National Science Foundation (MCB-0131659 and MCB-0132083). Additional support was provided by grants from the NIH National Center for Research Resources through the Kansas Biomedical Research Infrastructure Network (KBRIN; P20 RR16475), the Office of Research Administration at Wichita State University, the Mervin Bovaird Institute for Molecular Biology and Biotechnology at The University of Tulsa, and the Oklahoma EPSCoR program.

Author information

Authors and Affiliations

  1. Department of Biological Sciences, Wichita State University, Wichita, KS, 67260, USA

    T.M. Caton, L.R. Witte, H.D. Ngyuen & M.A. Schneegurt

  2. Department of Biological Sciences, University of Tulsa, Tulsa, OK, 74104, USA

    J.A. Buchheim & M.A. Buchheim

Authors
  1. T.M. Caton
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L.R. Witte
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H.D. Ngyuen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J.A. Buchheim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M.A. Buchheim
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M.A. Schneegurt
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M.A. Schneegurt.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Caton, T., Witte, L., Ngyuen, H. et al. Halotolerant Aerobic Heterotrophic Bacteria from the Great Salt Plains of Oklahoma. Microb Ecol 48, 449–462 (2004). https://doi.org/10.1007/s00248-004-0211-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00248-004-0211-7

Keywords

Search

Navigation

pFad - Phonifier reborn

Pfad - The Proxy pFad of © 2024 Garber Painting. All rights reserved.

Note: This service is not intended for secure transactions such as banking, social media, email, or purchasing. Use at your own risk. We assume no liability whatsoever for broken pages.


Alternative Proxies:

Alternative Proxy

pFad Proxy

pFad v3 Proxy

pFad v4 Proxy