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DNA synthesis and epigenetic modification during mouse oocyte fertilization by human or hamster sperm injection

  • Epigenetics
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

To evaluate DNA synthesis and epigenetic modification in mouse oocytes during the first cell cycle following the injection of human or hamster sperm.

Methods

Mouse oocytes following the injection of human and hamster sperm and cultured in M16 medium.

Results

Male and female pronucleus formation, DNA synthesis, histone protein modification, and heterochromatin formation were similar in mouse oocytes injected with human or hamster sperm. However, DNA methylation patterns were altered in mouse oocytes following human sperm injection. Immunocytochemical staining with a histone H3-MeK9 antibody revealed that human and hamster sperm chromatin associated normally with female mouse chromatin, then entered into the metaphase and formed normal, two-cell stage embryos.

Conclusions

Although differences in epigenetic modification of DNA were observed, fertilization and cleavage occurred in a species non-specific manner in mouse oocytes.

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Acknowledgements

This study was supported financially by a grant from the BioGreen 21 program (201003010612240010400 and 201003010612240010500) and KOSEF (20100002070), Rural Development Administration (RDA), Republic of Korea.

Author information

Authors and Affiliations

  1. Department of Animal Sciences, Chungbuk National University, Gaesin-dong, Cheongju, Chungbuk, 361-763, South Korea

    Yong-Nan Xu, Jin-Cheol Tae, Yong-Xun Jin & Nam-Hyung Kim

  2. College of Laboratory Animal, Jilin University, Changchun, Jilin Province, 130062, China

    Xiang-Shun Cui

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  1. Yong-Nan Xu
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Correspondence to Nam-Hyung Kim.

Additional information

Capsule Using mouse oocytes as ICSI recipients for human and mamster sperm, embryonic cell cycle progression is observed despite the presence of altered epigenetic modifications in sperm chromatin.

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Xu, YN., Cui, XS., Tae, JC. et al. DNA synthesis and epigenetic modification during mouse oocyte fertilization by human or hamster sperm injection. J Assist Reprod Genet 28, 325–333 (2011). https://doi.org/10.1007/s10815-010-9509-1

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