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Detection of β-lactamase encoding genes in feces, soil and water from a Brazilian pig farm

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Abstract

β-lactam antibiotics are widely used for the treatment of different types of infections worldwide and the resistance to these antibiotics has grown sharply, which is of great concern. Resistance to β-lactams in gram-negative bacteria is mainly due to the production of β-lactamases, which are classified according to their functional activities. The aim of this study was to verify the presence of β-lactamases encoding genes in feces, soil, and water from a Brazilian pig farm. Different β-lactamases encoding genes were found, including blaCTX-M-Gp1, blaCTX-M-Gp9, blaSHV, blaOXA-1-like, blaGES, and blaVEB. The blaSHV and blaCTX-M-Gp1 genes have been detected in all types of samples, indicating the spread of β-lactam resistant bacteria among farm pigs and the environment around them. These results indicate that β-lactamase encoding genes belonging to the cloxacillinase, ESBL, and carbapenemase and they have high potential to spread in different sources, due to the fact that genes are closely related to mobile genetic elements, especially plasmids.

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Acknowledgments

The authors thank J.D.D. Pitout (University of Calgary, Calgary, AB, Canada) for kindly providing the β-lactamase control strains used in this study.

Funding

This work was supported by the São Paulo Research Foundation—FAPESP [grant number 2015/18990-2].

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  1. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café S/N. Monte Alegre, Ribeirão Preto, SP, 14040-903, Brazil

    João Pedro Rueda Furlan & Eliana Guedes Stehling

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  1. João Pedro Rueda Furlan
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Correspondence to Eliana Guedes Stehling.

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Furlan, J.P.R., Stehling, E.G. Detection of β-lactamase encoding genes in feces, soil and water from a Brazilian pig farm. Environ Monit Assess 190, 76 (2018). https://doi.org/10.1007/s10661-017-6453-x

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