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. 1983 Sep 1;158(3):795–810. doi: 10.1084/jem.158.3.795

MRC OX-22, a monoclonal antibody that labels a new subset of T lymphocytes and reacts with the high molecular weight form of the leukocyte-common antigen

PMCID: PMC2187095  PMID: 6224884

Abstract

A mouse monoclonal antibody (MRC OX-22) is described that labels rat T cells which mediate graft-versus-host reactions and those responsible for the suppression of antibody synthesis in hosts undergoing these reactions. In contrast, most of the T cells that provide help for B cells are MRC OX-22 negative. These results, taken together with those published previously, demonstrate that the rat contains at least three phenotypically and functionally distinct subsets of T cells. The MRC OX- 22 antibody also labels all B cells, 50% of bone marrow cells, but only 2% of thymocytes. Of these latter cells about half are found at the edge of the medulla and the remainder are randomly distributed throughout the cortex and medulla. These findings lend support to the view that mature thymocytes leave the thymus at the cortico-medullary junction, and also suggest that both cortex and medulla may be sites where thymocytes mature. Biochemical studies showed that the MRC OX-22 antibody reacts with the high molecular weight form of the leukocyte- common antigen (L-CA). Comparison with data on human L-CA suggests that the molecular and antigenic heterogeneity of this set of glycoproteins has been conserved between rat and man.

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Selected References

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  1. Barclay A. N. The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology. 1981 Apr;42(4):593–600. [PMC free article] [PubMed] [Google Scholar]
  2. Brideau R. J., Carter P. B., McMaster W. R., Mason D. W., Williams A. F. Two subsets of rat T lymphocytes defined with monoclonal antibodies. Eur J Immunol. 1980 Aug;10(8):609–615. doi: 10.1002/eji.1830100807. [DOI] [PubMed] [Google Scholar]
  3. Brown W. R., Williams A. F. Lymphocyte cell surface glycoproteins which bind to soybean and peanut lectins. Immunology. 1982 Aug;46(4):713–726. [PMC free article] [PubMed] [Google Scholar]
  4. Bunce J. V., Mason D. W. The toleration of rat thymocytes to xenogeneic erythrocytes: kinetics of induction and recovery. Eur J Immunol. 1981 Nov;11(11):889–896. doi: 10.1002/eji.1830111108. [DOI] [PubMed] [Google Scholar]
  5. Cantor H., Boyse E. A. Functional subclasses of T-lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen. J Exp Med. 1975 Jun 1;141(6):1376–1389. doi: 10.1084/jem.141.6.1376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Coffman R. L. Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development. Immunol Rev. 1982;69:5–23. doi: 10.1111/j.1600-065x.1983.tb00446.x. [DOI] [PubMed] [Google Scholar]
  7. Dalchau R., Fabre J. W. Identification with a monoclonal antibody of a predominantly B lymphocyte-specific determinant of the human leukocyte common antigen. Evidence for structural and possible functional diversity of the human leukocyte common molecule. J Exp Med. 1981 Apr 1;153(4):753–765. doi: 10.1084/jem.153.4.753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dalchau R., Kirkley J., Fabre J. W. Monoclonal antibody to a human leukocyte-specific membrane glycoprotein probably homologous to the leukocyte-common (L-C) antigen of the rat. Eur J Immunol. 1980 Oct;10(10):737–744. doi: 10.1002/eji.1830101003. [DOI] [PubMed] [Google Scholar]
  9. Dallman M. J., Mason D. W., Webb M. The roles of host and donor cells in the rejection of skin allografts by T cell-deprived rats injected with syngeneic T cells. Eur J Immunol. 1982 Jun;12(6):511–518. doi: 10.1002/eji.1830120612. [DOI] [PubMed] [Google Scholar]
  10. Dyer M. J., Hunt S. V. Committed T lymphocyte stem cells of rats. Characterization by surface W3/13 antigen and radiosensitivity. J Exp Med. 1981 Oct 1;154(4):1164–1177. doi: 10.1084/jem.154.4.1164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ford W. L., Burr W., Simonsen M. A lymph node weight assay for the graft-versus-host activity of rat lymphoid cells. Transplantation. 1970 Sep;10(3):258–266. doi: 10.1097/00007890-197009000-00007. [DOI] [PubMed] [Google Scholar]
  12. Fukumoto T., McMaster W. R., Williams A. F. Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus. Eur J Immunol. 1982 Mar;12(3):237–243. doi: 10.1002/eji.1830120313. [DOI] [PubMed] [Google Scholar]
  13. Gahmberg C. G., Andersson L. C. Selective radioactive labeling of cell surface sialoglycoproteins by periodate-tritiated borohydride. J Biol Chem. 1977 Aug 25;252(16):5888–5894. [PubMed] [Google Scholar]
  14. Gahmberg C. G., Häyry P., Andersson L. C. Characterization of surface glycoproteins of mouse lymphoid cells. J Cell Biol. 1976 Mar;68(3):642–653. doi: 10.1083/jcb.68.3.642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Galfrè G., Milstein C. Preparation of monoclonal antibodies: strategies and procedures. Methods Enzymol. 1981;73(Pt B):3–46. doi: 10.1016/0076-6879(81)73054-4. [DOI] [PubMed] [Google Scholar]
  16. Gilman S. C., Rosenberg J. S., Feldman J. D. Membrane phenotype of the rat cytotoxic T lymphocyte. J Immunol. 1982 Sep;129(3):1012–1016. [PubMed] [Google Scholar]
  17. Hsiung L., Barclay A. N., Brandon M. R., Sim E., Porter R. R. Purification of human C3b inactivator by monoclonal-antibody affinity chromatography. Biochem J. 1982 Apr 1;203(1):293–298. doi: 10.1042/bj2030293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Hunt S. V., Fowler M. H. A repopulation assay for B and T lymphocyte stem cells employing radiation chimaeras. Cell Tissue Kinet. 1981 Jul;14(4):445–464. doi: 10.1111/j.1365-2184.1981.tb00551.x. [DOI] [PubMed] [Google Scholar]
  19. Hunt S. V., Mason D. W., Williams A. F. In rat bone marrow Thy-1 antigen is present on cells with membrane immunoglobulin and on precursors of peripheral B lymphocytes. Eur J Immunol. 1977 Nov;7(11):817–823. doi: 10.1002/eji.1830071114. [DOI] [PubMed] [Google Scholar]
  20. Mason D. W., Pugh C. W., Webb M. The rat mixed lymphocyte reaction: roles of a dendritic cell in intestinal lymph and T-cell subsets defined by monoclonal antibodies. Immunology. 1981 Sep;44(1):75–87. [PMC free article] [PubMed] [Google Scholar]
  21. Mason D. W. Subsets of T cells in the rat mediating lethal graft versus-host disease. Transplantation. 1981 Sep;32(3):222–226. doi: 10.1097/00007890-198109000-00008. [DOI] [PubMed] [Google Scholar]
  22. Mason D. W. The class of surface immunoglobulin on cells carrying IgG memory in rat thoracic duct lymph: the size of the subpopulation mediating IgG memory. J Exp Med. 1976 May 1;143(5):1122–1130. doi: 10.1084/jem.143.5.1122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Mason D. W., Williams A. F. The kinetics of antibody binding to membrane antigens in solution and at the cell surface. Biochem J. 1980 Apr 1;187(1):1–20. doi: 10.1042/bj1870001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Morris R. J., Williams A. F. Antigens on mouse and rat lymphocytes recognized by rabbit antiserum against rat brain: the quantitative analysis of a xenogeneic antiserum. Eur J Immunol. 1975 Apr;5(4):274–281. doi: 10.1002/eji.1830050412. [DOI] [PubMed] [Google Scholar]
  25. Parish C. R., Hayward J. A. The lymphocyte surface. III. Function of Fc receptor, C'3 receptor and surface Ig bearing lymphocytes: identification of a radioresistant B cell. Proc R Soc Lond B Biol Sci. 1974 Nov 19;187(1089):379–395. doi: 10.1098/rspb.1974.0083. [DOI] [PubMed] [Google Scholar]
  26. Rannie G. H., Donald K. J. Estimation of the migration of thoracic duct lymphocytes to non-lymphoid tissues. A comparison of the distribution of radioactivity at intervals following i.v. transfusion of cells labelled with 3H, 14C, 75Se, 99mTc, 125I and 51Cr in the rat. Cell Tissue Kinet. 1977 Nov;10(6):523–541. [PubMed] [Google Scholar]
  27. Reinherz E. L., Morimoto C., Fitzgerald K. A., Hussey R. E., Daley J. F., Schlossman S. F. Heterogeneity of human T4+ inducer T cells defined by a monoclonal antibody that delineates two functional subpopulations. J Immunol. 1982 Jan;128(1):463–468. [PubMed] [Google Scholar]
  28. Reinherz E. L., Schlossman S. F. The differentiation and function of human T lymphocytes. Cell. 1980 Apr;19(4):821–827. doi: 10.1016/0092-8674(80)90072-0. [DOI] [PubMed] [Google Scholar]
  29. Scheid M. P., Landreth K. S., Tung J. S., Kincade P. W. Preferential but nonexclusive expression of macromolecular antigens on B-lineage cells. Immunol Rev. 1982;69:141–159. doi: 10.1111/j.1600-065x.1983.tb00453.x. [DOI] [PubMed] [Google Scholar]
  30. Standring R., McMaster W. R., Sunderland C. A., Williams A. F. The predominant heavily glycosylated glycoproteins at the surface of rat lymphoid cells are differentiation antigens. Eur J Immunol. 1978 Dec;8(12):832–839. doi: 10.1002/eji.1830081203. [DOI] [PubMed] [Google Scholar]
  31. Trowbridge I. S. Interspecies spleen-myeloma hybrid producing monoclonal antibodies against mouse lymphocyte surface glycoprotein, T200. J Exp Med. 1978 Jul 1;148(1):313–323. doi: 10.1084/jem.148.1.313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Waldmann H. Conditions determining the generation and expression of T helper cells. Immunol Rev. 1977;35:121–145. doi: 10.1111/j.1600-065x.1977.tb00238.x. [DOI] [PubMed] [Google Scholar]
  33. White R. A., Mason D. W., Williams A. F., Galfre G., Milstein C. T-lymphocyte heterogeneity in the rat: separation of functional subpopulations using a monoclonal antibody. J Exp Med. 1978 Sep 1;148(3):664–673. doi: 10.1084/jem.148.3.664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Williams A. F., Galfrè G., Milstein C. Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: differentiation antigens of rat lymphocytes. Cell. 1977 Nov;12(3):663–673. doi: 10.1016/0092-8674(77)90266-5. [DOI] [PubMed] [Google Scholar]

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