Table 1.
Summary of the phenotypes of the Hsp90 mutants
| Hsp82 mutants | G1 arrest (α-factor)
|
G1 arrest (Ste 11ΔN)
|
FUS1-LacZ expression
|
FUS1-HIS3 expression
|
||||
|---|---|---|---|---|---|---|---|---|
| 30°C | 21°C | 30°C | 21°C | 30°C | 21°C | 30°C | 21°C | |
| Hsp82 wt | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| 10% Hsp82 wt | − | − | NA | NA | +/− | +/− | ND | ND |
| Hsp82 G313N | − | ++ | +/− | +++ | +/− | ++ | − | ++ |
| Hsp82 T525l | +++ | +++ | +++ | +++ | ND | ND | ND | ND |
| hHsp90β | − | − | +/− | +++ | +/− | +/− | − | − |
Note that this table also includes all the results presented in Figures 1, B and C, 2, and 3A, which were obtained by culturing cells at 30°C. ND, not done; NA, not applicable. +++, ++ and +/− indicate full, partial, and weak responses, respectively.