Figure 6. Crosslinking Reveals the Closed ATP and compact ADP Conformations in Human and Yeast Hsp90.
In (A) Hsp90α was incubated with AMPPNP and 0.005% glutaraldehyde crosslinker prior to negative staining. Aligned single images, reference free class averages and aligned 2D projections of the Hsc82:AMPPNP reconstruction are shown (right to left). Box sizes are 250 Å. Hsp90α (B) and yeast Hsc82 (C) incubated with ADP and crosslinker are shown as in (A) and compared to the HtpG:ADP reconstruction.