FIGURE 7.
Mitochondrial changes in PINK1 knockdown cells require Drp1
activity. Control or PINK1shRNA stable clonal cell lines were
co-transfected with GFP-Drp1-DN and with a mitochondrially targeted RFP
(mito-RFP), and images were analyzed for mitochondrial interconnectivity
(A) and mitochondrial elongation (B) as described under
“Experimental Procedures.” (Representative of three independent
experiments; 
, p
< 0.005 versus control cell line;
, p < 0.05
versus respective empty vector-transfected PINK1shRNA cell line;
n = 25-35 cells quantified/condition.) Control or PINK1shRNA stable
lines were co-transfected with HA-tagged Drp1-DN and with GFP-LC3 to label
AVs. C and D, the average number of GFP-LC3 puncta
(C) or the percent colocalization of GFP-LC3 puncta with mitochondria
(D) by confocal microscopy and image analysis (representative of
three independent experiments;
, p < 0.01
versus control; ,
p < 0.05 versus empty vector-transfected A4 or D14;
n = 25-30 cells analyzed/condition).