Figure 7. Implications for the inducible regulation of PRG-1 expression.
(A–E) BMMΦs were stimulated for 1 hour with LPS and analyzed by ChIP (NCoR, CoREST, HDAC1, HDAC3, p50). Error bars represent mean of triplicate values+/−SEM. (F) MEFs were stimulated with IL-1 for 1 hour and analyzed by RT-qPCR. This data and data from three published microarrays was graphed as the number of genes induced following stimulation in MEFs, HUVECs, or Keratinocytes of macrophage LPS-inducible PRG-I and PRG-II/SRGs. A list of analyzed genes appears in Supplemental Table 1. (G) MEFs were analyzed by ChIP (H3K4me3, H3K9Ac, S5P Pol II, Sp1). Data is graphed as the number of genes in MEFs that are similar in H3K4me3, H3K9Ac, S5P Pol II, or Sp1 status to those genes in macrophages for either PRG-I or PRG-II genes. (H) Model of LPS-induced PRG-I transcription (see text for description). Squares indicate acetylated residues, circles indicate methylated residues, and stars indicate phosphorylated residues.