Figure 2.
Analysis of replication fork dynamics and restart in CPT-treated WT and ΔFANCM cells. (A) Top—schematic of the fibre labelling experiment, below—replication fork rates shown as frequency distribution in WT DT40 and ΔFANCM cells treated with CPT for 20 min during the second labelling period. The x axis represents the ratio between the tract length of the first label (IdU) divided by the tract length of the second label (CldU). (B) Top—schematic of the fibre labelling experiment for analysis of replication fork recovery and representative images of actual fibres from WT and ΔFANCM cells, below—effect of 90 min exposure to CPT on replication fork activity in the absence (C) and presence of roscovitine (D). The bars represent mean values from three independent experiments ±s.e.m.