Fig. 2.
Predominant infiltration of CCR4+ eTreg cells into melanoma tissues. (A) CCR4 expression by melanoma-infiltrating T cells. CD4+ T cells from melanoma sites were fractionated into subpopulations based on the expression of CCR4, CD45RA, and FOXP3; CCR4 expression by each fraction was analyzed. Data from two representative patients are shown. (Right) Summary of MFI of CCR4 expression by FOXP3− or FOXP3+ cells (n = 9). (B) CCR4+CD4+ T cells from melanoma tissues (Pt #16) were depleted of CCR4+ T cells and then analyzed for the proportion of FOXP3hi eTreg cells. (Lower) Percentages of FOXP3hi cells among CD4+ T cells after CCR4+ cell depletion or nondepletion (n = 9). The numbers in A and B indicate the percentage of gated CD4+ T cells.