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A schematic of the single-cell RRBS (scRRBS) technique. Note the completion of all of the following steps within the same reaction tube: lysis of an individual cell, release of the naked double-stranded genomic DNA, spiking with lambda DNA, digestion of the genomic DNA using a restriction enzyme, end-repair and dA-tailing of the DNA fragments, ligation of the adaptors to the DNA fragments, and bisulfite conversion of the ligated DNA.
