Fig. 2. Analysis of 100 Single Cells from Polygenomic Breast Tumor.
(a) T10 was macro-dissected into 12 sectors, and nuclei were isolated from six sectors and flow-sorted by ploidy. FACS profiles show four distributions of ploidy (F1–F4) which were gated to isolate 100 single cells. (b) Neighbor-joining tree of integer copy number profiles showing four major branches of evolution (c) Phylogenetic tree of consensus profiles show the common ancestors and evolutionary distance between subpopulations. Integer copy number profiles from single cells are displayed below, and pie charts indicate the percentage of cells that constitute each subpopulation.