Figure 2.
erk1b RNAs from related species drive synthesis of unrelated proteins domains. Genomic sequences corresponding to exon7-intron7-exon8 of MAPK3 (erk1) were retrieved from Ensembl release 83. First line: extension of the introns and exons, separated by vertical bars. “stop” indicates the final of ERK1 and ERK1b coding sequences. Sequences were aligned by multalin program with identity matrix (Corpet, 1988). Alignments were performed also with Tcoffee and Clustal Omega with the same results (not shown). Rat (Rattus norvegicus), guinea pig (Cavia porcellus), rabbit (Oryctolagus cuniculus), kangaroo-rat (Dipodomys ordii), squirrel (Ictidomys tridecemlineatus), mouse (Mus musculus), platypus (Ornithorhynchus anatinus). Letters in red, amino-acids highly conserved for a position among the protein sequences; letters in blue, amino-acids showing limited conservation; letters in black, amino-acids showing no conservation in the aligned sequences. (A) The C-terminal sequences of rodents' ERK1 proteins are highly conserved; platypus'ERK1 protein sequence is highly similar to rodents' ERK1s. (B) Rodents' ERK1b proteins display no significant conserved protein motifs after exon7.