TBBPA effects on adipocyte differentiation and lipid accumulation in 8 day assays. 3T3-L1 cells were either grown in adipocyte maintenance media (uninduced) (A) or treated (induced) with MDI (B) for 8 days with and without TBBPA. DMSO: vehicle control, ROSI: 1 μM rosiglitazone positive control, and 10 μM TBBPA. Cells were co-stained with Hoechst (nuclei) and Nile red (lipid). High content capture of images using DAPI and FITC filters provided data on (C) mean percent adipocytes per well and (D) mean lipid area (μm2) per adipocyte per well (mean ± SEM, n = 4). Dash line: induced control mean. Dotted line: uninduced control mean. One-way ANOVA with multiple comparisons Dunnett’s post hoc test p < .05, * statistically significant over induced control, p < .05, and † statistically significant over uninduced vehicle control, p < .05.