Papers by Sawsan Elsonbaty
Benha Veterinary Medical Journal
This study was conducted to investigate the preventive effects of Chitosan-nanoparticles on dieth... more This study was conducted to investigate the preventive effects of Chitosan-nanoparticles on diethylnitrosamine (DEN) induced hepatotoxicity in albino rats. Forty male albino rats were divided into four groups: group (1): normal control, group (2): group (2): hepatotoxic rats administrated DEN orally (20 mg/kg b.wt/ day), group (3): rats receive chitosan-nanoparticles orally and group (4): Hepatotoxic rats were treated with chitosan-nanoparticles orally at a dose of (200 mg / kg bw/day). All animals were sacrificed at the end of experiment (12 weeks). The hepatotoxic effect of DEN was evidenced by elevation of serum Aspartate Aminotransferase, Alanine Aminotransferase, Alkaline Phosphatase, γ-Glutamyl transferase activities, blood Urea concentration and liver Malondialdehyde level. However serum albumin concentration and antioxidants biomarkers in liver tissue were markedly decreased. In conclusion, the administration of chitosan nanoparticles restored the DEN induced alterations in liver functions, promoted oxidative stress and antioxidant defense
Benha Veterinary Medical Journal, 2021
Curcumin medicinal applications are hindered by its well-known unstable metabolic state and also ... more Curcumin medicinal applications are hindered by its well-known unstable metabolic state and also insufficient absorption and bioavailability. In recent years, nano-based drug delivery system has effectively improved the aqueous solubility and bioavailability of curcumin. The current study was established to evaluate the protective and therapeutic effect of nanocurcumin (Nano-Cur) in hepatocellular carcinoma induced in rats. Fifty white albino rats were divided into 5 groups 10 rat of each. Group I served as negative control while group II received DEN with a daily dosage of (20 mg/kg body weight) orally by gavage during twelve weeks. Group III rats received Nano-Cur (20 mg/kg body weight) three days a week by gavage for eighteen weeks. Group IV rats received DEN as group II and treated with Nano-Cur as group III for six weeks. Group V received Nano-Cur as protector, for six weeks before DEN administration with continuous administration with Nano-Cur till the experiment end. Blood and tissue samples were collected and removed for determination of biochemical, antioxidant, cytokine and apoptotic marker p53 examination at the end of experiment. Based on the findings, DEN group indicated significantly increased serum liver function enzymes ALT, AST, ALP this was accompanied with significant decrease in liver antioxidant enzyme activities of (SOD and CAT) along with significant elevation in lipid peroxidation. DEN significantly elevated IL-6 and reduced apoptotic marker p53. The findings of this research indicated that Nano-cur has a anemaelioration effect against DEN carcinogenic effect.
Benha Veterinary Medical Journal, 2021
The purpose of this study was to look into the antitumor activity of Asparagus racemosus root (AR... more The purpose of this study was to look into the antitumor activity of Asparagus racemosus root (AR) against hepatocellular carcinoma (HCC) in male rats that had been induced experimentally. Fifty white albino rats were categorized randomly to five groups. Group I act as a negative monitor. Group II was given DEN by gavage at dose of (20 mg/kg b.w/day/12 weeks). Group III rats were given AR (200 mg/kg b.w/18 weeks) via gavage. Group IV rats were given DEN at dose of (20 mg/kg b.w/day/12 weeks and then ARat dose of 200 mg/kg b.w/ 6 weeks. Group V rats were given AR at dose of (200 mg/kg b.w / 6 weeks before administration of DEN dose of (20 mg/kg b.w/day/12 weeks) till end of experiment. According to the findings, DEN treatment increased serum liver function enzymes ALT, AST, and ALP while decreasing the activity of hepatic antioxidant enzymes (SOD and CAT). DEN raised IL-6 levels while decreasing the apoptotic marker p53. Asparagus supplementation prior to or after DEN administration significantly reduced DEN toxicity and mutagenic effects, particularly when used as a protective. According to the findings, Asparagus racemosus roots should be used as an antioxidant and anti-tumor.
Archives of Biochemistry and Biophysics
Dose-Response
Introduction Hepatic inflammation is considered key driver of hepatic tissue impairment.We aimed ... more Introduction Hepatic inflammation is considered key driver of hepatic tissue impairment.We aimed to explore the interaction of Halamphora coffeaeformis ( Amph.) with low dose ionizing γ radiation ( γR) exposure against D-galactosamine (D-GaIN)-induced chronic hepatitis in Albino rats. Methods Chronic hepatitis was induced with single dose of D-GalN (400 mg/kg BW i.p.). Rats received 400 mg Amph/kg BW daily by gastric gavage concomitant with .25 Gy γ-R. Liver oxidative stress and inflammatory status were assessed. Gene expression levels of signal transducer and activator of transcription 3 (STAT3) and nuclear factor kappa B (NFKB) were estimated by q-PCR. D-Galactosamine injection significantly encouraged hepatic oxidative damage and inflammatory disturbance accompanied with improved intercellular adhesion molecule-1 level (ICAM-1). Results messenger RNA gene expression levels of STAT3 and NF-kB were expressively higher in D-GaIN-treated animals. Histopathological examination support...
Diabetes, when uncontrolled, causes dyslipidemia often followed by atherogenic abnormalities. The... more Diabetes, when uncontrolled, causes dyslipidemia often followed by atherogenic abnormalities. The present study was aimed to determine the effect of ferulic acid (FA) in diabetes-induced dyslipidemia. Diabetes in male rats induced by streptozotocin and treated by ferulic acid (FA) significantly reduced the elevated blood glucose and lipid profile levels. Thirty two waster male rats divided into four equal groups of 8 rats. Group Ι (Control group): received no drugs, Group Π (Ferulic acid group): received (10mg/kg body weight/day) orally for 21 days. Group IIΙ (Streptozotocin (STZ) group): received intramuscular injection once by STZ (40mg/kg body weight). Group VΙ :( Streptozotocin+Ferulic acid group (STZ+FA)): received intramuscular injection once by STZ (40mg/kg body weight) and after 3 days received orally ferulic acid (10mg/kg body weight/day) daily. Treatment in groups for 21 days after diabetes induction blood samples and pancreatic tissue were collected at 22th day from treat...
Biological Trace Element Research, 2022
Alzheimer's disease (AD) is a brain disorder associated with a gradual weakening in neurocogn... more Alzheimer's disease (AD) is a brain disorder associated with a gradual weakening in neurocognitive functions, neuroinflammation, and impaired signaling pathways. Resveratrol (RSV) has neuroprotective properties, but with low bioavailability, and low solubility in vivo. Selenium (Se) is an essential micronutrient for brain function. Thus, this study aimed to evaluate the role of formulated RSV-Se nanoparticles (RSV-SeNPs) on neurochemical and histopathological approaches associated with the AD model in rats induced by Aluminum chloride (AlCl3) at a dose of 100 mg/kg/day for 60 days. RSV-SeNPs supplementation attenuates the impaired oxidative markers and mitochondrial dysfunction. The ameliorative effect of RSV-SeNPs on cholinergic deficits was associated with clearance of amyloid β (Aβ). Furthermore, activation of phosphatidylinositol 3 kinase (PI3K) deactivates glycogen synthase kinase 3 beta (GSK-3β)-mediated tau hyperphosphorylation. Additionally, RSV-SeNPs downregulate signal transducer and activator of transcription (STAT3) expression as well as interleukin-1β (IL-1β) levels, therefore alleviating neuroinflammation in AD. Moreover, RSV-SeNPs upregulate the expression of Sirtuin-1 (SIRT1) and lower that of microRNA-134, consequently increasing neurite outgrowth. Eventually, the obtained results showed that nano-formulation of resveratrol with selenium maximized the therapeutic potential of RSV against Alzheimer's disease not only by their antioxidant but also by anti-inflammatory effect improving the neurocognitive function and modulating the signaling pathways.
Journal of Genetic Engineering and Biotechnology
Background Synthesized gallium nanoparticles synthesized by grape seed extract were characterized... more Background Synthesized gallium nanoparticles synthesized by grape seed extract were characterized with spherical shape and size range less than100 nm, possessing the functional groups of the biological material. The purpose of this study is to evaluate gallium nanoparticles synthesized by grape seed extract, as an antitumor agent with low dose of γ-radiation against hepatocellular carcinoma in rats. Aim of work This work aimed to evaluate the antitumor effect of gallium nanoparticles synthesized (GaNPs) by grape seed extract and the co-binded treatment with low dose of γ-radiation on hepatocellular carcinoma in rats, through evaluating their effect on signaling pathways and tumor markers. Results Cytotoxic activity of GaNPs synthesized by grape seed extract was estimated by mediated cytotoxicity assay on HepG2 cell line that recorded IC50 of 388.8 μg/ml. To achieve these goals, eighty Wistar male rats (120−150 g) will be divided into eight groups, each of 10 rats. The animals are ad...
Journal of Radiation Research and Applied Sciences, 2014
The small intestinal epithelium is highly sensitive to radiation and a major site of injury durin... more The small intestinal epithelium is highly sensitive to radiation and a major site of injury during radiation therapy and environmental overexposure. The objective of this study was to evaluate yerba mate extract (MT) as a potential radioprotective agent in the intestine. First yerba mate was exposed to doses of gamma radiation and the extract was analyzed for its antimicrobial, antioxidant and total phenolic compounds. Male mice were divided into 4 groups: Control group: received saline, MT group: received MT, Irradiation group: exposed to 8 Gy of gamma radiation, MTþ irradiation group: pre-treated with MT then exposed to gamma radiation. The results obtained in vitro showed that, MT of gamma irradiated yerba mate showed a reduction in antioxidant activity and total phenolic compounds compared to control. MT exerted antimicrobial activity with minimum inhibitory concentration (0.5 mg/ml) for Lactobacillus, Escherichia coli, Staphylococcus aureus and Streptococcus faecalis. In vivo, mice exposed to gamma radiation, showed significant reduction in the total microbial count and disappearance of E. coli, induction of lipid peroxidation, protein carbonyl, advanced oxidation protein products, and a decrease in glutathione, also decrease in the activity of lysozyme, catalase and superoxide dismutase, induction in cytokine levels of TNF-a and IL-6 with DNA fragmentation in the intestine, while irradiated mice pre-treated with MT showed, normalized lactic acid bacteria count and diminished pathogenic bacteria as S. aureus and E. coli also showed ameliorative effect of MT on lipid peroxidation, glutathione, protein carbonyl, advanced oxidation protein products, also normalized the activity of lysozyme, catalase and superoxide dismutase, and ameliorated cytokine levels of TNF-a and IL-6 compared to control, with radioprotection effect on the DNA of intestinal tissue. Our data suggested that, pretreatment with MT may protect the small intestine from gamma radiation damaging effects showed in the measured biochemical parameters also maintained the microbial flora of lactic acid bacteria and removed pathogenic bacteria and may replace antibiotics description for radiotherapy patients since it showed antimicrobial effect against pathogenic bacteria.
Antioxidants and hepatoprotective effects of chitosan nanoparticles against hepatotoxicity induced in rats, 2019
This study was conducted to investigate the preventive effects of Chitosan-nanoparticles on dieth... more This study was conducted to investigate the preventive effects of Chitosan-nanoparticles on diethylnitrosamine (DEN) induced hepatotoxicity in albino rats. Forty male albino rats were divided into four groups: group (1): normal control, group (2): group (2): hepatotoxic rats administrated DEN orally (20 mg/kg b.wt/ day), group (3): rats receive chitosan-nanoparticles orally and group (4): Hepatotoxic rats were treated with chitosan-nanoparticles orally at a dose of (200 mg / kg bw/day). All animals were sacrificed at the end of experiment (12 weeks). The hepatotoxic effect of DEN was evidenced by elevation of serum Aspartate Aminotransferase, Alanine Aminotransferase, Alkaline Phosphatase, γ-Glutamyl transferase activities, blood Urea concentration and liver Malondialdehyde level. However serum albumin concentration and antioxidants biomarkers in liver tissue were markedly decreased. In conclusion, the administration of chitosan nanoparticles restored the DEN induced alterations in ...
Innovative bioactive hydrogel dressings based on Psyllium (Pys) and Carbopol (Carp) supported wit... more Innovative bioactive hydrogel dressings based on Psyllium (Pys) and Carbopol (Carp) supported with Frankincense essential oil (FO) were fabricated using γ- irradiation facility for crosslinking and sterilization. (Psy-Carp)/FO dressing’s characteristics, hemolytic potential, antioxidant efficiency, antimicrobial activity as well as their morphological features were evaluated. Wound healing efficacy was evaluated using a rat model. The results revealed that the gelation degrees increased by the increase of (Psy) concentration, Carbopol content, and irradiation dose. The incorporation of FO oil up to 5% improves the swelling ability as well as the dressing porosity as shown by SEM images. The incorporation of FO oil enhances the Water Vapor Transmission Rate (WVTR), increases the antioxidant activity. Antimicrobial activity and microbial barrier potency of (Psy-Carp)/FO dressing was tested against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and Candida albicans (C. ...
Synthesis of nanoparticles that have environmentally acceptable solvent systems and eco-friendly ... more Synthesis of nanoparticles that have environmentally acceptable solvent systems and eco-friendly reducing agents is of great importance. The aim of this work was to synthesis of silver nanoparticles (AgNPs) using Spirulina platensis and Nostoc sp. at room temperature. UV-visible spectrometry study revealed surface plasmon resonance at 402 nm. The transmission electron micrographs of nanoparticles in an aqueous solution showed the production of AgNPs (average size of most particles˜11.5 nm by Spirulina platensis and 20.3 nm by Nostoc sp.). The Fourier Transmittance Infrared spectrum (FT-IR) confirms the presence of biocomponent in the algae which was responsible for the nanoparticles synthesis. The antibacterial activity silver nanoparticles against both gram positive bacteria (Staphylococcus aureus, Staphylococcus epidedymis) and gram negative bacteria (Klebsiella pneumonia, Escherichia coli and Pseudomonas aeruginosa) by well diffusion method showed excellent antibacterial activity against all tested bacterial. This green process gives the greater potential biomedical applications of silver nanoparticles.
Curcumin medicinal applications are hindered by its well-known unstable metabolic state and also ... more Curcumin medicinal applications are hindered by its well-known unstable metabolic state and also insufficient absorption and bioavailability. In recent years, nano-based drug delivery system has effectively improved the aqueous solubility and bioavailability of curcumin. The current study was established to evaluate the protective and therapeutic effect of nanocurcumin (Nano-Cur) in hepatocellular carcinoma induced in rats. Fifty white albino rats were divided into 5 groups 10 rat of each. Group I served as negative control while group II received DEN with a daily dosage of (20 mg/kg body weight) orally by gavage during twelve weeks. Group III rats received Nano-Cur (20 mg/kg body weight) three days a week by gavage for eighteen weeks. Group IV rats received DEN as group II and treated with Nano-Cur as group III for six weeks. Group V received Nano-Cur as protector, for six weeks before DEN administration with continuous administration with Nano-Cur till the experiment end. Blood an...
The purpose of this study was to look into the antitumor activity of Asparagus racemosus root (AR... more The purpose of this study was to look into the antitumor activity of Asparagus racemosus root (AR) against hepatocellular carcinoma (HCC) in male rats that had been induced experimentally. Fifty white albino rats were categorized randomly to five groups. Group I act as a negative monitor. Group II was given DEN by gavage at dose of (20 mg/kg b.w/day/12 weeks). Group III rats were given AR (200 mg/kg b.w/18 weeks) via gavage. Group IV rats were given DEN at dose of (20 mg/kg b.w/day/12 weeks and then ARat dose of 200 mg/kg b.w/ 6 weeks. Group V rats were given AR at dose of (200 mg/kg b.w / 6 weeks before administration of DEN dose of (20 mg/kg b.w/day/12 weeks) till end of experiment. According to the findings, DEN treatment increased serum liver function enzymes ALT, AST, and ALP while decreasing the activity of hepatic antioxidant enzymes (SOD and CAT). DEN raised IL-6 levels while decreasing the apoptotic marker p53. Asparagus supplementation prior to or after DEN administration ...
Arab Journal of Nuclear Sciences and Applications, Apr 1, 2020
Medicinal plants providing natural products may be used as precursors for new drugs for their che... more Medicinal plants providing natural products may be used as precursors for new drugs for their chemical diversity. In the present study, the potential of gamma radiation as sterilizing agent for preservation and its effect on phytochemical contents, antioxidant and antibacterial activity of Teucrium polium (T. polium) was analyzed for antimicrobial activity. The results revealed that exposing plant powder to doses of gamma irradiation (5, 10, 15& 20 kGy) significantly increased the ethyl acetate extraction yield (dry weight) as compared to the non-irradiated plant (control). Teucrium polium phytochemical contents of total phenolic content (TPC), total flavonoids content (TFC) and tannins content were significantly increased with gamma irradiation. In the same line, the antioxidant activity was significantly increased in response to radiation dose. Teucrium polium showed antibacterial activity against Pseudomonas aeroginosa and Staphylococcus aureus. Gamma irradiation significantly increased the antibacterial activity against P. aeroginosa and S. aureus at dose of 20 KGy compared to other doses. The microbial load total count of T. polium was significantly reduced by exposure to gamma radiation (5, 10, 15 & 20 kGy). Exposure to 5.0 kGy significantly reduced the bacterial count by 99% while fungi count was not detected, while exposure to 10 kGy was enough to eliminate bacteria and fungi from plant sample.
Environmental Science and Pollution Research
Environmental and occupational exposure to aluminum along with ionizing radiation results in seri... more Environmental and occupational exposure to aluminum along with ionizing radiation results in serious health problems. This study was planned to investigate the impact of oxidative stress provoked by exposure to ionizing radiation with aluminum administration upon cellular ultra structure and apoptotic changes in Paneth cells of rat small intestine. Animals received daily aluminum chloride by gastric gavage at a dose 0.5 mg/Kg BW for 4 weeks. Whole body gamma irradiation was applied at a dose 2 Gy/week up to 8 Gy. Ileum malondialdehyde, advanced oxidation protein products, and protein carbonyl were assessed as biomarkers of lipid peroxidation along with superoxide dismutase, catalase, and glutathione peroxidase activities as enzyme antioxidants. Moreover, analyses of cell cycle division and apoptotic changes were evaluated by flow cytometry. Intestinal cellular ultra structure was investigated using transmission electron microscope. Oxidative stress assessment in the ileum of rats revealed that aluminum and ionizing radiation exposure either alone or in combination exhibits a significant effect upon the increase in biomarkers of lipid peroxidation along with tumor necrosis factor-α with concomitant significant decrease of the antioxidant enzyme activities. Flow cytometric analyses showed significant alterations in the percentage of cells during cell cycle division phases along with significant increase in apoptotic cells. Ultra structurally, intestinal cellular alterations with marked injury in Paneth cells at the sites of bacterial translocation in the crypt of lumens were recorded. The results of this study have clearly suggested that aluminum exposure and ionizing either alone or in combination induced apoptosis and oxidative stress in the Paneth cells of rat intestine, which appeared to play a major role in the pathogenesis of cellular damage. Furthermore, the interaction of these two intestinal toxic routes was found to be synergistic.
Environmental Science and Pollution Research
European Journal of Medicinal Plants, 2016
Benha Veterinary Medical Journal
β-glucans are heterogeneous group of natural polysaccharides mostly investigated for their immuno... more β-glucans are heterogeneous group of natural polysaccharides mostly investigated for their immunological effects. The objective of this study was to evaluate the efficiency of irradiated β-glucan on Nnitrosodiethylamine induced hepatocarcinogenesis in rats. β-glucan powder was exposed to 50 KGY of gamma radiation for experimental study, healthy male rats were divided in five groups. Group 1: did not receive any treatment. Group 2: hepatic cancer group rats administrated with DEN orally at a dose of (20mg/kg b.wt/day) for six weeks. Group 3: Irradiated β-glucan group rats were orally administrated with Iβ-glucan for six weeks at a dose of 65 mg/kg body weight/day. Group 4: Protective group rats were administrated Iβ-glucan daily as in group 3 for two weeks they induced with DEN concomitant with Iβglucan for six weeks. Group 5: Therapeutic group rats were induced daily with DEN then administrated with Iβ-glucan for six weeks. DEN induced liver damage as evidence by a marked increase in serum indices of liver marker enzymes (Alt, Ast and GGT), tumor markers (alpha fetoprotein) and proinflammatory cytokines(interleukin-6) when compared with the normal control group. However, administration of irradiated β-glucan, in liver cancer induced rats displayed improvement in all measured parameters. In conclusion Iβ-glucan considered as a preventive and a therapeutic towards hepatocarcinogenesis via enhancing immune system in particular CD4, CD8 count and anti-inflammatory effect.
Archives of Biochemistry and Biophysics
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Papers by Sawsan Elsonbaty