POMBOX MoClo YTK
(Kit #
1000000251
)
Depositing Lab: Tomáš Pluskal
POMBOX is a collection of cloning parts for Golden Gate Assembly-based plasmid assembly in Schizosaccharomyces pombe. POMBOX follows the Modular Cloning Yeast Toolkit (MoClo-YTK) grammar and is compatible with its parts. This plasmid kit provides 14 promoters, six synthetic terminators, two loci of integration, and six additional pairs of connectors.
This kit will be sent as bacterial glycerol stocks in 96-well plate format.
Original Publication
POMBOX: A Fission Yeast Cloning Toolkit for Molecular and Synthetic Biology. Hebra T, Smrčková H, Elkatmis B, Převorovský M, Pluskal T. ACS Synth Biol. 2024 Feb 16;13(2):558-567. doi: 10.1021/acssynbio.3c00529. PubMed (Link opens in a new window) Article (Link opens in a new window).
Description
POMBOX allows for seamless and efficient assembly of single-gene and multigene plasmids for genome integration in Schizosaccharomyces pombe. POMBOX is fully compatible with all DNA parts that have been designed following the MoClo-YTK grammar. POMBOX can also be used as a standalone kit as it provides pre-assembled cassettes of parts 1, 5, 6, 7, 8 from the MoClo-YTK grammar and functional promoters and terminators of S. pombe.
Figure 1: POMBOX provides six pairs of connectors providing up to 12 transcriptional units (parts 1 and 5), 14 characterized promoters (part 2), six synthetic terminators plus six S. cerevisiae terminators (part 4), selection markers, tags, and other parts from MoClo-YTK (parts 3, 6, and 8), and 2 loci of integration (parts 7 and 8b). Image reused from (Hebra et al. 2024) under CC-BY 4.0 (Link opens in a new window) license.
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The POMBOX MoClo YTK was a gift from Tomáš Pluskal (Addgene kit #1000000251).”
For your Reference section:
POMBOX: A Fission Yeast Cloning Toolkit for Molecular and Synthetic Biology. Hebra T, Smrčková H, Elkatmis B, Převorovský M, Pluskal T. ACS Synth Biol. 2024 Feb 16;13(2):558-567. doi: 10.1021/acssynbio.3c00529. PubMed (Link opens in a new window) Article (Link opens in a new window).
POMBOX MoClo YTK - #1000000251
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Chloramphenicol | |
| Ampicillin | |
| Spectinomycin |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| A / 1 | pPOM001_P1_ConL6 | Chloramphenicol |
| A / 2 | pPOM002_P1_ConL7 | Chloramphenicol |
| A / 3 | pPOM003_P1_ConL8 | Chloramphenicol |
| A / 4 | pPOM004_P1_ConL9 | Chloramphenicol |
| A / 5 | pPOM005_P1_ConL10 | Chloramphenicol |
| A / 6 | pPOM006_P1_ConL11 | Chloramphenicol |
| A / 7 | pPOM007_P5_ConR6 | Chloramphenicol |
| A / 8 | pPOM008_P5_ConR7 | Chloramphenicol |
| A / 9 | pPOM009_P5_ConR8 | Chloramphenicol |
| A / 10 | pPOM010_P5_ConR9 | Chloramphenicol |
| A / 11 | pPOM011_P5_ConR10 | Chloramphenicol |
| A / 12 | pPOM012_P5_ConR11 | Chloramphenicol |
| B / 1 | pPOM013_P2_pENO101 | Chloramphenicol |
| B / 2 | pPOM014_P2_pGPM1 | Chloramphenicol |
| B / 3 | pPOM015_P2_pADH1 | Chloramphenicol |
| B / 4 | pPOM016_P2_pRPL2501 | Chloramphenicol |
| B / 5 | pPOM017_P2_pTIF51 | Chloramphenicol |
| B / 6 | pPOM018_P2_pRPL701 | Chloramphenicol |
| B / 7 | pPOM019_P2_pRPL2102 | Chloramphenicol |
| B / 8 | pPOM020_P2_pTRS1 | Chloramphenicol |
| B / 9 | pPOM021_P2_pTUB1 | Chloramphenicol |
| B / 10 | pPOM022_P2_pRPS1002 | Chloramphenicol |
| B / 11 | pPOM023_P2_pAPL4 | Chloramphenicol |
| B / 12 | pPOM024_P2_pMPA1 | Chloramphenicol |
| C / 1 | pPOM025_P2_pTUP11 | Chloramphenicol |
| C / 2 | pPOM026_P2_pNMT1 | Chloramphenicol |
| C / 3 | pPOM034_P7_3"Lys3 | Chloramphenicol |
| C / 4 | pPOM040_P8b_5'Ura4 | Chloramphenicol |
| C / 5 | pPOM036_P7_3"Ura4 | Chloramphenicol |
| C / 6 | pPOM027_P4_tSynthGuo | Ampicillin |
| C / 7 | pPOM028_P4_tSynth3 | Ampicillin |
| C / 8 | pPOM029_P4_tSynth25 | Ampicillin |
| C / 9 | pPOM030_P4_tSynth27 | Ampicillin |
| C / 10 | pPOM031_P4_tSynth29 | Ampicillin |
| C / 11 | pPOM032_P4_tSynth30 | Ampicillin |
| C / 12 | pPOM038_P8b_5'Lys3 | Ampicillin |
| D / 1 | pPOM041_P15678_SingleGeneBackbone | Ampicillin |
| D / 2 | pPOM042_P15678_MultiGeneBackbone | Spectinomycin |