Ana Kristina Gasteyer was born on May 4, 1967 to Marian Roumell-Gasteyer and Phil Gasteyer. Gasteyer began her career at Northwestern University; she initially enrolled as a voice major, but later switched to theater studies when she started to get involved with the campus' improv comedy group. She went on to further develop and foster her comedy work with the Los Angeles improv group, The Groundlings.

Gasteyer is perhaps best known for her iconic work on Saturday Night Live. During her six-year stint, she created and delivered some of the most iconic SNL characters, including middle school music teacher "Bobbie Moughan-Culp", NPR radio host "Margaret Jo", Lilith Fair poetess "Cinder Calhoun", as well as her incomparable impressions of Martha Stewart, Celine Dion and Hillary Rodham Clinton.

On the big screen, Gasteyer was most recently seen in Amy Poehler's feature directorial debut, Wine Country, opposite her SNL friends and colleagues, currently available on Netflix. Next up for Gasteyer is Clea Duvall's Sony picture, Happiest Season, which she co-stars opposite Kristen Stewart, Aubrey Plaza and Victor Garber.

On the small screen, Gasteyer is a recurring character on the hit ABC series, The Goldbergs, as well as on the show's spin-off, Schooled. Additional notable credits include comedy hits Netflix's Lady Dynamite, TBS' People of Earth, HBO's Curb Your Enthusiasm, and ABC's Suburgatory.

In addition to her comedy work, Gasteyer is an accomplished singer and songwriter. Her freshman album, I'm Hip, was released in 2014. It received critical reviews. Gasteyer's most recent album, Sugar & Booze, topped numerous "Best Holiday Album" lists. Billboard called the album "an uproarious homage to Christmas albums of old". Continuing, the LA Times wrote that, "'Saturday Night Live' alumna Gasteyer puts her considerable vocal chops to work here to marvelous effect on this ebullient big-band jazz effort."

Inspired by the music from Sugar and Booze, Gasteyer produced an 8-episode original series for Audible, which she co-wrote with Mona Mansour. The series featured characters voiced by Gasteyer, Maya Rudolph, Patti Lupone, and Rachel Dratch, among others.

Gasteyer was able to marry her phenomenal vocal talents with her acting skills in two of Fox's live musicals A Christmas Story and the iconic musical Grease. As well as Showtime's Reefer Madness. Most recently, she dazzled on the enormous hit Fox musical series The Masked Singer as fan favorite, The Tree.

On the stage, Gasteyer originated the role of "Elphaba" in the Chicago sit-down of Wicked and then went on to play the role on Broadway. Her resume also includes The Rocky Horror Show and the Tony Nominated plays: The Royal Family and Three Penny Opera. She also starred in Funny Girl, and Passion at The Chicago Shakespeare Theater, which earned her a Jefferson Award nomination. She played Miss Hannigan in the Tony-winning musical Annie at The Hollywood Bowl.