Wow, this is an oldie, and sometimes it looks it. It was my first look, actually, at a Mutt and Jeff cartoon but I remember reading them in comic fashion as a kid for many years.
Anyway, they are cowboys and apparently a team in entertaining folks, perhaps, in a rodeo because we see them practicing their act. Mutt is a little rough on Jeff, making his dance by shooting the ground and then whipping him!
I didn't find much humor is this cartoon, and I hope other Mutt and Jeff's are better than this. I realize it was silent movie era but the noises the two guys made in place of talking (whistles and hoots) was a little annoying. I'd rather they used title cards or "balloons with words" than those dumb incoherent noises.
The color in this movie is fair, about what you'd expect for something re- released in the 1930s under the "Kromcolor" process. The artwork is pretty primitive but, hey, it's 80 years old!