On the DVD short documentary, Claude Rains' daughter Jessica Rains tells of a time when her father brought her to see a re-release of this movie in the theater in Pennsylvania in 1950. It was bitterly cold and his face was completely covered by a hat and scarf. When he spoke to ask for the tickets, the attendant immediately recognized his voice and wanted to let them in for free. Rains was quite upset at this and demanded that he pay full price.

Though the music at the pub comes from a coin-operated player piano, it, along with everyone talking in the pub, stops short at the startling arrival of the Invisible Man.

Claude Rains is the only actor in the film whose character is identified in the credits. The roles the other actors play are not identified, even though the cast is listed twice: at the beginning and at the end. Rains is billed as "The Invisible One" in the opening credits and as "The Invisible Man" in the closing credits.

When the film was released to home video, Universal Studios replaced a snippet of music heard on the radio when Dr. Kemp is reading a newspaper in his house, and the Invisible Man enters through a set of French doors. Universal was unable to secure the rights for the original music and replaced it, covering the original sound effects (the sound of the newspaper and the door latch) in the process. The original music and missing sound effects were restored to the 2012 blu-ray audio.

Featured in Sherlock Holmes and the Voice of Terror (1942)