The theme song for this show was composed by Paul Francis Webster and Bob Harris. The theme song was recorded at RCA Studios in Toronto (where the cartoon was also produced) featuring 12 CBC vocalists (members of the Billy Van Singers, and Laurie Bower Singers groups) who added to the musical backing track supplied by RCA Studios, New York. The singers were paid only for the session and have had no residuals from its use since then. The song became as synonymous with Spider-Man as his red and blue costume.

Only over 40 issues of "The Amazing Spider-Man" comic magazines were completed at the time of this cartoon's development.

This version of the Green Goblin is more like Stan Lee's original idea for the character. Lee's original synopsis for the Green Goblin had a movie crew, on location, finding an Egyptian-like sarcophagus. Inside was an ancient mythological demon, the Green Goblin who naturally came to life. This was not used in the comics because Spider-Man co-creator Steve Ditko changed Lee's idea and made the Green Goblin a human villain. Ditko was not involved with this cartoon, but Stan Lee was a story consultant.

In this series Spider-Man's costume only has webbed areas on his head, arms and boots; the rest is plain, except for spiders on his chest and back. It's assumed that this made him easier to animate.

Though the character movement was greater than what was used on The Marvel Super Heroes (1966), the series was produced on a budget and relied on reused stock animation, including Spider-Man swinging across the New York City skyline and Peter stripping off his white dress shirt to reveal his spider suit. Character movement was also minimized.