Supplementary Figure 9: Evaluating the effect of different conditions on the transcriptome.
From: Multiplexed quantification of proteins and transcripts in single cells
(a) The REAP-seq transcriptomic assay was run on PBMCs labeled with or without AbBs (scRNA-seq). Sequencing reads were processed with our bulk RNA-seq pipeline (Omicsoft, see Methods) and the 5,000 highest expressed genes were compared between the two conditions. High correlation (R2=0.97) suggests that labeling cells with AbBs does not effect the transcriptomic signature. (b) PBMCs were either blocked with dextran sulfate (0.2 mg/ml) or not blocked before labeling cells with AbBs and processed through the REAP-seq pipeline. Comparison between the top 5,000 expressed genes show good correlation (R2=0.96) suggesting that blocking cells with dextran sulfate does not effect the transcriptomic signature. Gene counts were normalized by total counts and scaled by a factor of 1×106.
