A-B. Frequency of unique reads in each possible barcode combination in the human (hg38 noAlt) and mouse (mm10 noAlt) datasets. C-D. Frequency of percent clonal reads in each possible barcode combination in the human (hg38 noAlt) and mouse (mm10 noAlt) datasets. Clonal read statistics were rounded to the nearest whole percent before calculating. E. Collision density plot showing the ratio of unique reads mapped to human and unique reads mapped to mouse in each unique barcode combination (data with <500 reads were excluded). See Methods for determination of which data sets are considered collisions. F. Theoretical and experimentally derived collision rate percentages. Note that collision rates cannot be directly measured for mouse or human only nuclei. G. Violin plot of unique read counts for nuclei that pass filter and nuclei that pass filter with multi-mappers removed. The blue dots represent average number of unique reads and the red dots represent the median number of unique reads. H. Violin plot as in (G) showing only unique read count range of 0 to 80,000. I. Visualization of merged single cell data and individual single cell datasets from the visual cortex. All unique reads that passed filter were combined to generate the merged tract. Single cell datasets that had reads present in the chromosome 17 locus from 8,100,000 to 8,250,000 bp were randomly sampled and plotted in the single cell tracts section. Each black dot represents a unique read. UCSC genes are overlaid at the bottom.