Flow cytometric analysis of CD24 expression on Siglec-H⁺Ly6C⁻, Siglec-H⁺Ly6C⁺, Siglec-H⁻Ly6C⁻ or Siglec-H⁻Ly6C⁺ pre-DC subsets in BM, blood and spleen (representative data shown of 3 independent experiments with a total of 7 individual mice, (a)) (a). Representative scanning electron microscopy images of individual cells from each pre-DC subset following purification from the bone marrow by FACS (b). Flow cytometric analysis of intensity of GFP expression in ZBTB46, ID2 and CX3CR1 reporter mice in Siglec-H⁺Ly6C⁻, Siglec-H⁺Ly6C⁺, Siglec-H⁻Ly6C⁻ or Siglec-H⁻Ly6C⁺ pre-DC subsets from mouse BM, number depicts mean fluorescent intensity (MFI) (representative data shown of 3 independent experiments with a total of 2 mice per strain per experiment, (c)). Frequency of pre-DC subsets among total pre-DCs in BM, blood and spleen (data representative of 3 independent experiments with 3 mice per experiment, mean ± SEM, (d)). Flow cytometric analysis of Ly6C and Siglec-H expression on the progeny of CD45.2⁺ pre-DC subsets (CD45.2⁺Siglec-H⁺Ly6C⁻, CD45.2⁺Siglec-H⁺Ly6C⁺, CD45.2⁺Siglec-H⁻Ly6C⁺ or CD45.2⁺Siglech-H⁻Ly6C⁻ pre-DCs) added individually into day 2 in vitro CD45.1⁺ FLT3L-stimulated BM cultures. Cells depicted were harvested on day 3 and gated for Lin⁻B220⁻MHCII⁻CD45.2⁺CD11c⁺ expression (data representative of 2 independent experiments with 1 analyte per population, mean ± SEM, (e)).