Supplementary Figure 3: Target-specific in situ detection of gRNA molecules expressed by RNA polymerase III. | Nature Methods

Supplementary Figure 3: Target-specific in situ detection of gRNA molecules expressed by RNA polymerase III.

From: Rapidly evolving homing CRISPR barcodes

Supplementary Figure 3

(a) A schematic of our in situ amplification and detection assay based on FISSEQ. (b) Results of target-specific in situ amplification and detection for two different hgRNA constructs and a negative control. The schematic on top shows the position of the reverse-transcription (RT) primer in each design. The bottom panels show a representative field of view from each of two biological replicates. Amplicons are labeled with Cy5 (orange) and nuclei are labeled with DAPI (blue).

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