Supplementary Figure 4: Replicate analyses and cell-line comparisons.
From: Combinatorial CRISPR–Cas9 screens for de novo mapping of genetic interactions
Scatterplot of fitness (fg) for single gene knockouts for biological replicates in (a) HeLa, (b) A549, and (c) 293T cells. Scatterplot of raw genetic interaction scores (π gg') for biological replicates in (d) HeLa, (e) A549, and (f) 293T cells. The significant interactions underlying the correlation calculation are highlighted in bold. (g) Genes that have large fitness effects when they are disrupted have greater number of genetic interactions (node degree, includes positive interactions as well as synthetic lethal interactions). This was true for Hela (blue), A549 (pink) and 293T (black) cells. Interaction hubs (genes with highest node degree) are highlighted for each cell line. (h) Scatterplot of fitness (fg) for single gene knockouts in HeLa versus 293T. (i) Scatterplot of fitness (fg) for single gene knockouts in A549 versus 293T. (j) Scatterplot of interaction scores in HeLa versus 293T. (k) Scatterplot of interaction scores in A549 versus 293T.
