Supplementary Figure 8: Cell-type-restricted expression and sparse labeling in the enteric nervous system using AAV-PHP.S together with neuron-specific gene regulatory elements or Cre transgenic mice. | Nature Neuroscience

Supplementary Figure 8: Cell-type-restricted expression and sparse labeling in the enteric nervous system using AAV-PHP.S together with neuron-specific gene regulatory elements or Cre transgenic mice.

From: Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous systems

Supplementary Figure 8

Images show XFP expression within the myenteric plexus. (a) ssAAV-PHP.S:hSyn1-3XFP was administered intravenously at a total dose of 1 × 1012 vg. (b) ssAAV-PHP.S:CAG-DIO-3XFP was administered intravenously at a total dose of 1 × 1012 vg to ChAT-IRES-Cre transgenic mice to achieve cell type-specific expression in cholinergic neurons. (c) Sparse labeling with the VAST system in the proximal colon of a ChAT-IRE-Cre transgenic using ssAAV-PHP.S-TET-DIO-3XFP at 1 × 1012 vg and ssAAV-PHP.S-ihSyn-DIO-tTA at 1 × 1010 vg. Color-coded square boxes display neurons traced manually in Imaris v8.3. (d) Rectangular boxes show higher magnification views highlighting how sparse multicolor labeling facilitates tracing of closely aligned processes. Double arrows mark traced processes. Scale bars in (a and d) are set to 50 μm and in (c) to 200 μm.

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