Extended Data Fig. 2: Knockout of twinfilins does not affect cellular levels of actin and actin-binding proteins.
From: Twinfilin uncaps filament barbed ends to promote turnover of lamellipodial actin networks
Identical amounts of lysates of wild-type and twf1/twf2 KO B16-F1 cells were loaded on SDS-PAGE gels and protein levels were determined with Western blot. a, Levels of β-actin. Anti-α-tubulin antibody was used as a loading control. We note that anti-β-actin antibody remained in the blot even after vigorous stripping of the membrane and was thus visible also in anti-α-tubulin detection. Protein levels of b, Capping Protein, c, cofilin-1, d, profilin-1, e, cyclase-associated protein-1, and f, CARMIL-1. Anti-α-tubulin or anti-histone H3 antibodies were used as a loading controls. All Western blot experiments were repeated three times, except in panel c where experiment was repeated two times. Unprocessed Western blots are provided in Source Data Extended Data Fig. 2.
