Extended Data Fig. 4: Analysis of stable-isotope feeding experiments.
From: Host metabolism balances microbial regulation of bile acid signalling
a, Administration of taurine-d4 in SPF mice resulted in deuterium incorporation in all detected taurine conjugates, but not in any MCY conjugates. Shown are EICs for the m/z of molecular ions of unlabeled (black) and deuterium-labeled versions (red) of the different conjugates in serum of mouse fed taurine-d4. b, Administration of deuterium-labeled L-cysteine (L-cys-d2) in SPF mice resulted in deuterium incorporation in the MCY conjugates of BAs. Shown are EICs for the m/z of the molecular ions of the unlabeled (black) and the deuterium-labeled versions of BA-MCY conjugates (red) detected in serum of mice fed L-cys-d2. c,d, Administration of deuterium-labeled L-cysteine (L-cys-d2) in SPF mice (see Fig. 2i) resulted in deuterium incorporation in taurine conjugates of BAs (c) and pantetheine (d). EICs for molecular ion peaks (black) and deuterium isotope peaks (red) of taurine conjugates of BAs (c) and pantetheine (d) in serum of mouse fed L-cys-d2.
