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    LB Antibody Plates

    Uploaded by

    Jackie White
    The document provides instructions for making LB antibiotic plates in two sizes - 1000mL and 500mL. For the 1000mL plates, the recipe calls for 10g tryptone, 5g yeast extract, 10g NaCl, 15g agar, and 1mL of kanamycin antibiotic at a concentration of 100mg/μL. For the 500mL plates, the recipe uses 5g tryptone, 2.5g yeast extract, 5g NaCl, 7.5g agar and 0.5mL of kanamycin. The solutions are autoclaved, cooled, antibiotics added, and poured into plates.

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    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd

    LB Antibody Plates

    Uploaded by

    Jackie White
    19 views1 page
    The document provides instructions for making LB antibiotic plates in two sizes - 1000mL and 500mL. For the 1000mL plates, the recipe calls for 10g tryptone, 5g yeast extract, 10g NaCl, 15g agar, and 1mL of kanamycin antibiotic at a concentration of 100mg/μL. For the 500mL plates, the recipe uses 5g tryptone, 2.5g yeast extract, 5g NaCl, 7.5g agar and 0.5mL of kanamycin. The solutions are autoclaved, cooled, antibiotics added, and poured into plates.

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    The document provides instructions for making LB antibiotic plates in two sizes - 1000mL and 500mL. For the 1000mL plates, the recipe calls for 10g tryptone, 5g yeast extract, 10g NaCl, 15g agar, and 1mL of kanamycin antibiotic at a concentration of 100mg/μL. For the 500mL plates, the recipe uses 5g tryptone, 2.5g yeast extract, 5g NaCl, 7.5g agar and 0.5mL of kanamycin. The solutions are autoclaved, cooled, antibiotics added, and poured into plates.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    19 views1 page

    LB Antibody Plates

    Uploaded by

    Jackie White
    The document provides instructions for making LB antibiotic plates in two sizes - 1000mL and 500mL. For the 1000mL plates, the recipe calls for 10g tryptone, 5g yeast extract, 10g NaCl, 15g agar, and 1mL of kanamycin antibiotic at a concentration of 100mg/μL. For the 500mL plates, the recipe uses 5g tryptone, 2.5g yeast extract, 5g NaCl, 7.5g agar and 0.5mL of kanamycin. The solutions are autoclaved, cooled, antibiotics added, and poured into plates.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
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    LB Antibody plates: 1000mL 10g BActo-Tryptone 5g Bacto-yeast extract 10g NaCl 15g Bacto-agar ddH20 to 1 L 500mL 5g BActo-Tryptone 2.

    e 2.5g Bacto-yeast extract 5g NaCl 7.5g Bacto-agar ddH20 to 1 L

    Autoclave to sterilize Cool to 55C Add antibiotic: Concentration 0.1mg/L Kanamycin 100mg/L 0.5mL (500mL) / 1mL (1000mL) Poor ~ 20 plates per 500mL

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