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    Biochemical Test For Identification and Differentiations of Enterobacteriaceae

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    Vijaya Subramani
    This document describes various biochemical tests used to identify and differentiate Enterobacteriaceae bacteria, including MacConkey agar, EMB agar, Hektoen enteric agar, ONPG test, TSI agar, indole test, methyl red test, Voges-Proskauer test, citrate test, lysine and ornithine decarboxylase tests, urea test, phenylalanine deaminase test, gelatinase test, and motility test. Each test examines bacterial properties or enzymatic reactions related to lactose fermentation, gas and H2S production, indole formation, acid production, acetoin formation, citrate utilization, decarboxylation of amino

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    Biochemical Test For Identification and Differentiations of Enterobacteriaceae

    Uploaded by

    Vijaya Subramani
    116 views17 pages
    This document describes various biochemical tests used to identify and differentiate Enterobacteriaceae bacteria, including MacConkey agar, EMB agar, Hektoen enteric agar, ONPG test, TSI agar, indole test, methyl red test, Voges-Proskauer test, citrate test, lysine and ornithine decarboxylase tests, urea test, phenylalanine deaminase test, gelatinase test, and motility test. Each test examines bacterial properties or enzymatic reactions related to lactose fermentation, gas and H2S production, indole formation, acid production, acetoin formation, citrate utilization, decarboxylation of amino

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    Biochemical Tests

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    Biochemical Tests

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    This document describes various biochemical tests used to identify and differentiate Enterobacteriaceae bacteria, including MacConkey agar, EMB agar, Hektoen enteric agar, ONPG test, TSI agar, indole test, methyl red test, Voges-Proskauer test, citrate test, lysine and ornithine decarboxylase tests, urea test, phenylalanine deaminase test, gelatinase test, and motility test. Each test examines bacterial properties or enzymatic reactions related to lactose fermentation, gas and H2S production, indole formation, acid production, acetoin formation, citrate utilization, decarboxylation of amino

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    116 views17 pages

    Biochemical Test For Identification and Differentiations of Enterobacteriaceae

    Uploaded by

    Vijaya Subramani
    This document describes various biochemical tests used to identify and differentiate Enterobacteriaceae bacteria, including MacConkey agar, EMB agar, Hektoen enteric agar, ONPG test, TSI agar, indole test, methyl red test, Voges-Proskauer test, citrate test, lysine and ornithine decarboxylase tests, urea test, phenylalanine deaminase test, gelatinase test, and motility test. Each test examines bacterial properties or enzymatic reactions related to lactose fermentation, gas and H2S production, indole formation, acid production, acetoin formation, citrate utilization, decarboxylation of amino

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    Biochemical test for identification

    and differentiations of
    Enterobacteriaceae
    MacConky agar
    Bile salts
    Crystal violet
    Lactose
    Neutral red
    Lac + pink or red
    Lac not colored
    Leviness EMB agar
    Bile salts
    Eosin and methylene
    blue precipitate at low
    PH
    Lactose + dark
    nucleus
    Lactose pale
    Hektoen enteric agar
    Selective for sal. & sh.
    Bile salts
    Bromthymol blue
    Acid fuchsin
    Sodium thiosulfate
    Ferric ammonium citrate
    Lac + pink to orange
    Lac blue green
    H2S ferric sulfide
    ( black precipitate)
    ONPG test
    0-nitrophenyl--
    galactopyranoside
    Tow enzyme : permease
    and -galactosidase
    Lactose _____ glucose +
    galactose
    Slow fermenters only have
    -galactosidase
    ONPG+ -
    galactosidase(hydrolysis)
    O-nitrophenol(yellow)
    Triple sugar iron agar(TSI)
    Lactose, glucose and
    sucrose
    Ferrous sulfate
    Phenol red
    Reveal gas and H2S
    production
    H2S+ iron salts_____
    black precipitate
    ( ferric sulfide)
    Indole test
    Tryptophan ________
    acid pyruvic
    +ammonia+Indole
    Kovacs reagent
    (HCl+ p-
    dimethylaminobenzald
    ehyde+n-amyl
    alcohol)
    tryptophanase
    Methyl red (MR) test
    Glucose fermentation(
    PH=4.2) acid
    formation
    Methyl red dye
    remain red when
    adding in the tube
    MR negative bacteria
    yellow to orange
    Voges-Proskauer(VP)
    Determine whether the
    product of glucose is
    acetylmethylcarbinol(acet
    oin) neutral metabolite
    Acetoin reacts with
    Barrits reagent(KOH &
    creatinine) to form a pink
    to rosy red tinge in the
    medium
    In negative result the tube
    remains brown to yellow
    Citrate media
    Contain citrate as the only
    usable carbon source
    Ammonium dihydrogen
    phosphate
    Bromthymolblue(indicator
    )
    By using citrate alkaline
    by-products
    The color of medium
    from green( neutral) to
    blue(alkaline)
    Lysine and ornithine
    decarboxylase(LDC, ODC)
    Enzymes that remove
    carboxyl from aminoacids
    The end products are
    alkaline amines
    The raise of PH convert
    bromcresol purple to
    purple color
    Negative results are
    yellow
    Urea medium
    2% Urea
    Phenol red
    Urea __________
    ammonia +CO2
    The indicator turns
    bright pink
    urease
    Phenylalanine(PA) deaminase
    Remove an amino
    group from PA
    Produce
    phenylpyruvic acid
    Visualized by adding
    ferric chloride10%
    Indicator turns olive
    green
    Gelatinase test
    Below 32 semisolid
    Above 32 viscous
    Motility
    Hanging drop slide
    Motility test medium

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