Acid and Base Lab2
Acid and Base Lab2
Acid and Base Lab2
The student will determine the pH of various substances and will also
determine how some of these substances can affect the environment.
Materials
pH paper and chart
well plate
pipettes
various household solutions
Procedure
1. Put a drop of each substance, one per well on the well plate. Remember, do
NOT come in contact with any of the materials and do not mix-n-match
pipettes.
2. Using your knowledge of the pH scale, hypothesize whether the liquid
would be an acid or base. Record this number on your data table.
3. Pick up a piece of pH paper with dry hands.
4. Touch the pH paper to the liquid in well A and remove it. Compare the
color of the paper with that on the pH chart. Record your observations on the
chart below.
5. Repeat the procedure with the rest of the slides.
Clean-up
1.
2.
3.
4.
Data
ID
A
B
C
D
E
F
G
H
I
J
K
L
M
Item
Hypothesis
Base
Acid
Acid
Base
Acid
Acid
Base
Base
Base
Base
Sugar Water
Baking Soda and Water
Salt Water
Borax
Vinegar
Ketchup
Dishwashing Liquid
Apple juice
Milk
Tap water
pH
Acid
Base
Neutral
7
9
6
9
2
3
8
4
7
7
Analysis
1. Which of the liquids had the lowest pH?
Vinegar
2. Which of the liquids had the highest pH?
Dishwashing Liquid
3. Which of the liquid(s) were closest to being neutral?
Salt Water and Dishwashing Liquid
4. If the pH of a sample was 3, how many times more acidic is it than a solution with a pH
of 6?
The sample would be 1000 times more acidic than the solution with the pH of 6.
5. How might one correct the pH of a lake with a reading of 3? Explain your reasoning.
You can correct the pH of the lake by trying to neutralizing it, so it can be balanced.
Since it has the pH of 3 then its very acidic, so to balance it you need to add more
base to the lake.
Biological Applications of pH:
1. What is the pH of human blood?
The pH of human blood is 7.4.
2. What would happen to our bodies if blood pH was changed or altered?
3. The pH of stomach acid is 2, why is this both good, but possibly destructive to our bodies?
The stomach acid in our bodies can be good because it can make your food particles
smaller, but it can also be bad for our bodies because too much of pH in the stomach acid
can affect the other systems in our bodies.
4. What could happen to our local environment if a company pumped materials into the
atmosphere which created a drastic pH change in the rain water?
It will make the people around the environment sick and they could die as well.
5. When we eat too many tomato products (ex. Salsa) our stomachs can become upset. Using
your understanding of pH and the pH chart in your text, why does an antacid make you feel
better?