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    Lab Report

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    This lab report details experiments testing traditional Chinese herbal medicines against E. coli bacteria. Five herbs were prepared at different concentrations in fresh and stored non-fresh forms. The herbs were Huo Xiang, Jin Yin Hua, Bai Zhu, Dang Shen, and Gan Jiang. E. coli was inoculated with the herbal solutions and incubated. Results found that none of the herbal concentrations, whether fresh or non-fresh, were able to kill the E. coli bacteria. The experiments thus showed these particular herbal medicines in the tested concentrations were not effective treatments for E. coli infections.

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    Lab Report

    Uploaded by

    api-282851608
    69 views4 pages
    This lab report details experiments testing traditional Chinese herbal medicines against E. coli bacteria. Five herbs were prepared at different concentrations in fresh and stored non-fresh forms. The herbs were Huo Xiang, Jin Yin Hua, Bai Zhu, Dang Shen, and Gan Jiang. E. coli was inoculated with the herbal solutions and incubated. Results found that none of the herbal concentrations, whether fresh or non-fresh, were able to kill the E. coli bacteria. The experiments thus showed these particular herbal medicines in the tested concentrations were not effective treatments for E. coli infections.

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    This lab report details experiments testing traditional Chinese herbal medicines against E. coli bacteria. Five herbs were prepared at different concentrations in fresh and stored non-fresh forms. The herbs were Huo Xiang, Jin Yin Hua, Bai Zhu, Dang Shen, and Gan Jiang. E. coli was inoculated with the herbal solutions and incubated. Results found that none of the herbal concentrations, whether fresh or non-fresh, were able to kill the E. coli bacteria. The experiments thus showed these particular herbal medicines in the tested concentrations were not effective treatments for E. coli infections.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    69 views4 pages

    Lab Report

    Uploaded by

    api-282851608
    This lab report details experiments testing traditional Chinese herbal medicines against E. coli bacteria. Five herbs were prepared at different concentrations in fresh and stored non-fresh forms. The herbs were Huo Xiang, Jin Yin Hua, Bai Zhu, Dang Shen, and Gan Jiang. E. coli was inoculated with the herbal solutions and incubated. Results found that none of the herbal concentrations, whether fresh or non-fresh, were able to kill the E. coli bacteria. The experiments thus showed these particular herbal medicines in the tested concentrations were not effective treatments for E. coli infections.

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    Lab Report

    Material:
    Equipment

    Bottle
    Beaker
    Plate
    Graduate cylinder

    Reagent

    Escherichia coli (strain B/BL21(DE3))


    Clove oil
    Tryptone
    Yeast Extraction
    Bacteriological Agar
    NaCl
    Huo Xiang
    Jin Yin Hua
    Bai Zhu
    Dang Shen
    Gan Jiang

    Procedure:
    Part I: Preparation Media
    1. Total media amount is 750mL which consist of 500mL of agar and 250mL of broth
    2. Weigh all the reagent:
    a. Tryptone 10g
    b. Yeast extract 5g
    c. NaCl 10g
    d. Agar 7.5g
    e. Water 750mL
    3. Pour tryptone, yeast extract and NaCl together into 1000mL of bottle flask.
    4. Add 750mL of water into 1000mL of bottle flask and swirl until it mixed.
    5. Then pour 250mL into 250mL of bottle for broth and 500mL into 500mL of bottle for agar
    6. For agar, add 7.5g of agar into 500mL of bottle.
    7. Cut the aluminum foil and wrap on both the bottle.
    8. Setting the autoclave machine on liquid.
    9. Place both agar bottle and broth bottle into autoclave when autoclave was setting.
    10. Remove the solution after finished and allow the agar to cool to 55C.
    11. Keep the area sterile when pouring the plate by working near the flame or Bunsen burner.
    12. Measure the agar 30mL for each plate by using sterile graduated cylinder.

    Part II: Inoculation E.coli


    1. Sterile working area by using 70 percent of Ethanol
    2. Using flame or Bunsen burn while working on the inoculation E.coli.
    3. Using 15 mL of centrifugal tube and approximately 2 mL of Broth to inoculate with BL21DE3
    Competent E.coli
    4. Then store in the incubator for 18 hours.
    Part III: Preparation Medicine
    Part I: Not fresh medicine
    1. Weight the amount of each herb:
    Sample

    Huo Xiang
    Jin Yin Hua
    Bai Zhu
    Dang Zhen
    Gan Jiang

    Weight
    Concentration Concentration Concentration
    1
    2
    3
    5g
    7.5g
    10g
    9g
    12g
    15g
    3g
    9g
    15g
    6g
    10g
    14g
    3g
    6g
    10g

    Volume of
    water
    200mL
    300mL
    100mL
    *
    combination
    of three herb

    2. Label name and the amount of concentration on each 600mL of beaker.


    3. Put each sample into each 600mL of beaker except Bai Zhu , Dang Zhen and Gan Jiang put in
    the same beaker by following the table.
    4. Add water into 600mL of beaker, follow the volume from the table.
    5. Place on them on the hot plate then boiling for 40-60 minute.
    6. Store all sample in the refrigerator for 1 week.
    Part II: Fresh medicine
    1. Weight the amount of each herb

    2.
    3.
    4.
    5.

    Weight
    Volume of water
    Sample
    Concentration 1
    Concentration 2
    Huo Xiang
    18g
    21g
    200mL
    Jin Yin Hua
    15g
    30g
    200mL
    Bai Zhu
    18g
    28g
    200mL
    * combination of
    Dang Zhen
    20g
    20g
    three herb
    Gan Jiang
    12g
    32g
    Repeat weight of concentration 1 from not fresh table.
    Label name and concentration on the 600mL of beaker.
    Put the sample into each 600mL of beaker and mix Bai Zhu, Dang Zhen and Gan jiang in the
    same beaker by following the table.
    Place on the hot plate and boil them for 40-60 minute.

    Result
    Not fresh medicine with BL21DE3 Competent E.coli:

    Concentration1

    Concentration 2

    Concentration 3

    Fresh medicine with BL21DE3 Competent E.coli:

    Concentration1

    New Concentration 1

    New Concentration 2

    Discussion
    The experiment was substantiate the traditional medicine with E.coli. The concentration was different in
    each sample. Including three kind of herb, such as Huo Xiang, Jin Yin Hua and combination of Bai Zhu,
    Dnag Shen and Gan Jiang. These were popular Chinese herb that used to prevent from diarrhea, nausea,
    poor appetite and fever, it was similar infection from E.coli symptom. The quality of Chinese medicine
    was freshness, it was important to patient.

    According to research, the characteristic of Chinese medicine was a liquid and also required to take it
    when medicine was warming. The experiment was concerned about fresh or not fresh medicine, it was
    depend on the time that had taken. To compare freshness and not freshness, these were separated
    extraction in two part. The first part would be keep for 1 week after completing. The other part would
    be used immediately after extraction was finished. The concentration in fresh part would be comprise
    two concentration, such as concentration one that was same as non-fresh concentration one too. And
    the new concentration that dissimilar with other sample.
    Throughout the experiment, we had two single and three combination herb. We changed the amount of
    herb in every concentration and water. The volume of water specified from the highest weight of each
    herb. In order to the concentration of each herb from greatest to smallest, the weight of Huo Xiang was
    5g ,7.5g and 10g per 200 mL of water, Jin Yin Hua was 9g, 12g and 15g per 300mL of water and the last
    one was three combination were 12g, 25g and 49g, all in fresh part. Non-fresh part, the new
    concentration of Huo Xian , Jin Yin Hua and three combination increased serially to 18g and 21g, 15g and
    30g, and 50g and 80g.
    When the herb solution was completed, the process was ready for demonstration with E.coli. The
    number of solution would be use about 110mml in each sample. This experiment would be test for five
    time in each concentration, to examine precision of the solution with E.coli.
    From the experiment, there were some error during the inoculation the agar, we changed some of
    ingredient to Iodized refined salt, instead of NaCl because our laboratory did not have them. Secondly,
    we begin with the non-fresh medicine. There were some of agar plate had been contract a disease that
    was unusable for proving. As the result, all the sample cannot kill the bacteria then, we had a new
    prediction from quality medicine had been reduced. That why we had the second part for support our
    purpose.

    Conclusion
    We would see that all the medicine sample cannot kill the bacteria. We could prove that all these
    sample were not use for patient who infect E.coli even the action was to prevent symptom that similar
    as symptom of E.coli.

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