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    SOLUTIONS Supplemental Practice Problems 2012

    Uploaded by

    Khaled Abeed
    This document provides examples of calculations for preparing solutions of various concentrations from stock solutions. It also includes examples of determining concentrations in reaction mixtures or samples based on known volumes and dilutions used. The key steps shown are: 1) Using the formula C1V1=C2V2 to determine the volume of stock solution needed to make a given volume of a desired concentration 2) Calculating mass of solute needed based on molarity, molecular weight and volume for concentration expressions 3) Working through dilution calculations to determine original concentrations in samples based on volumes and dilutions used.

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    © All Rights Reserved
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    SOLUTIONS Supplemental Practice Problems 2012

    Uploaded by

    Khaled Abeed
    61 views2 pages
    This document provides examples of calculations for preparing solutions of various concentrations from stock solutions. It also includes examples of determining concentrations in reaction mixtures or samples based on known volumes and dilutions used. The key steps shown are: 1) Using the formula C1V1=C2V2 to determine the volume of stock solution needed to make a given volume of a desired concentration 2) Calculating mass of solute needed based on molarity, molecular weight and volume for concentration expressions 3) Working through dilution calculations to determine original concentrations in samples based on volumes and dilutions used.

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    SOLUTIONS Supplemental Practice Problems 2012

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    This document provides examples of calculations for preparing solutions of various concentrations from stock solutions. It also includes examples of determining concentrations in reaction mixtures or samples based on known volumes and dilutions used. The key steps shown are: 1) Using the formula C1V1=C2V2 to determine the volume of stock solution needed to make a given volume of a desired concentration 2) Calculating mass of solute needed based on molarity, molecular weight and volume for concentration expressions 3) Working through dilution calculations to determine original concentrations in samples based on volumes and dilutions used.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    61 views2 pages

    SOLUTIONS Supplemental Practice Problems 2012

    Uploaded by

    Khaled Abeed
    This document provides examples of calculations for preparing solutions of various concentrations from stock solutions. It also includes examples of determining concentrations in reaction mixtures or samples based on known volumes and dilutions used. The key steps shown are: 1) Using the formula C1V1=C2V2 to determine the volume of stock solution needed to make a given volume of a desired concentration 2) Calculating mass of solute needed based on molarity, molecular weight and volume for concentration expressions 3) Working through dilution calculations to determine original concentrations in samples based on volumes and dilutions used.

    Copyright:

    © All Rights Reserved
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    SOLUTIONS:Practiceproblems2012

    1. Howwouldyouprepare400mlofa0.24MNaClsolution(MW=58.44g/mole)?
    [VxCxMW)0.4Lx0.24moles/Lx58.44g/mole=5.61gNaCldissolvedin/broughtupto400mlwater

    2. Howwouldyouprepare750mlofa0.35MNa2PO4solution(MW=141.96g/mole)?
    0.75Lx0.35moles/Lx141.96g/mole=37.265gNa2PO4dissolvedin/broughtupto750mlwater

    3. Howwouldyouprepare225mlofa0.15Mcitricacidsolution(453.6g/mole)?
    0.225Lx0.15moles/Lx453.6g/mole=15.309gcitricaciddissolvedin/broughtupto225mlwater

    4. Whatvolumeofstock0.24MNaClisneededtoprepare75mlof0.1MNaCl?
    (cv=cv)(0.24M)(V1)=(0.1M)(75ml)V1=(0.1M)(75ml)/(0.24M)=31.25mlofstockNaCl

    5. Whatvolumeofstock0.35MNa2PO4isneededtoprepare150mlof0.2MNa2PO4?
    V1=(0.2M)(150ml)/(0.35M)=85.714mlofstockNa2PO4

    6. Whatvolumeofstock0.15Mcitricacidisneededtoprepare230mlof2.5x103Mcitricacid?
    V1=(0.0025M)(230ml)/(0.15M)=3.833mlofstockcitricacid

    7. Howmuch(ml)3x103MNa2PO4canbepreparedfrom20mlof0.2MNa2PO4?
    [cv=cv]solveforV2:V2=(0.2M)(20ml)/0.003M=1333.33ml

    8. Ifyouhave35mlof0.1MNaCl,isitenoughtoprepare200mlof0.06MNaCl?
    [cv=cv]solveforV1:V1=((0.06M)(200ml)/(0.1M)=120ml,sono,youdonothaveenoughstock.,OR,usinga
    differentapproach,
    SolveforV2:V2=((0.1M)(35ml)/(0.06m)=58.333mlstockneededtomakethisamount.
    9. Foreachofthefollowing,indicatethemass(g)ofreagentneededtoprepare125mloftheindicatedpercent
    solution:

    Reagent
    Molecularweight Percentsolutionneeded(125ml) Massofreagent(g)
    NaCl
    58.44
    10%
    (0.1g/ml)(125ml)=12.5g
    Na2PO4
    141.96
    4.5%
    (0.045g/ml)(125ml)=5.625g
    Citricacid 453.6
    2.5%
    (0.025g/ml)(125ml)=3.125g
    Percentconcentrationisastraightmass/volume(g/ml)calculationanddoesntneedtheMW.1g/ml=100%solution.

    10. Youhave200mlof50XTAE.Whatvolumeofthe50XTAEisneededtoprepare25mlof10XTAE?
    Cv=cv:solveforV1:V1=((10X)(25ml)/(50X)=5mlof50XTAEstock

    11. Aprotocolcallsforusingaworkingconcentrationof1.5X104Mboricacid.Tosaveshelfspace,youdecideto
    makeupa20Xstocksolution.Whatwillbethemolarityofthatstocksolution?
    20x1.5x104M=3.0x103M

    12. Youmakeup1000mlofabuffercontaining300mlcitricacidand700mldisodiumphosphate(Na2PO4).Ifthe
    citricacidstocksolutionis0.15MandtheNa2PO4stocksolutionis0.35M,whataretheworkingconcentrations
    ofthesetwocomponentsinthebuffer?
    Citricacid=(300ml/1000ml)x(0.15M)=0.045Mor4.5x102M
    Na2PO4=(700ml/1000ml)x(0.35M)=0.245Mor2.45x101M

    13. Youprepareacrudeextractofalphaamylasefrom2.7gofgerminatedbarleyseedin10mlofbuffersolution.
    Whatistheextractconcentrationintermsofmilligramsoftissueextractedpermilliliterofbuffer?
    2.7g/10ml=0.27g/mlor270mg/ml

    14. Youuse0.5mloftheextractpreparedin#13inatotalreactionvolumeof6ml.Whatistheworking
    concentrationoftheextractinthereaction?
    (0.5ml)(270mg/ml)/6ml=22.5mg/ml
    15. InaplatecountanalysisofE.colicelldensityinawatersampleyoufind57coloniesonanLBagarplate
    inoculatedwith100ulof104dilutedwatersample.Whatisthecelldensity(#cells/ml)ofE.coliinthewater
    sample?
    Given:1colonyarisesfrom1cell
    (57cells/100ul)x10=570cells/mlinthe104dilution(thisnormalizestocells/ml)
    570cells/mlx104=5.7x106cells/ml=theoriginalcelldensityinthewatersample.

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