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    Home-Made HBsAg

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    somchais
    This short article described a simple experiment which tested the antigenic properties of the antigen of hepatitis B virus which was prepared in our lab.

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    Download as PDF, TXT or read online from Scribd

    Home-Made HBsAg

    Uploaded by

    somchais
    82 views1 page
    This short article described a simple experiment which tested the antigenic properties of the antigen of hepatitis B virus which was prepared in our lab.

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    Home-made HBsAg

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    © Attribution Non-Commercial (BY-NC)

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    This short article described a simple experiment which tested the antigenic properties of the antigen of hepatitis B virus which was prepared in our lab.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    82 views1 page

    Home-Made HBsAg

    Uploaded by

    somchais
    This short article described a simple experiment which tested the antigenic properties of the antigen of hepatitis B virus which was prepared in our lab.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    Home-made Hepatitis B Surface Antigen

    S. Saengamnatdej, Ph.D.
    August 19, 2010

    Our pure positive antigen for testing hepatitis B viral infection was running out
    and I need certain amount of it in a next couple of days. We knew that we had some
    peripheral blood donated from a carrier in the University Hospital kept in a -20 °C
    freezer.
    We therefore tried making the antigen from the blood. The protocol that we used
    was very easy and the antigenic property of the antigen was tested.

    Protocol
    A. Preparation of HBsAg
    1. Thaw the frozen blood completely at room temperature.
    2. Heat it in boiling water for 5 minutes.
    3. Remove it from the water bath and leave it to cool down a bit.
    4. Spin in a centrifuge at 3000xg for 5 minutes.
    5. Decant the clear solution into new eppendorfs

    B. Testing the antigen


    1. Apply 50 microlitres of the antigen (step 5, above) onto the pad of a test strip
    for detection of HBsAg.
    2. Wait for 5 minutes and read the result.

    C. Result
    There were two bands on the test strip; one was the control (the
    upper one) and the other was the test band (see the picture on the
    right) of the current antigen.

    D. Discussion and Conclusion


    The antigen was intended to be used in the demonstration and
    training a student in a course, so we needed to make very sure that the
    virus and all those infectious or non-infectious bio-hazards in the blood
    were completely inactivated. This would make the handle of the
    antigen much easier. The blood cells and proteins turned to pale gray
    which suggested that they were denatured and had lost their native
    biological activities during the heating step. The surface protein of the
    hepatitis B virus would be denatured in some level as well, but
    fortunately, as tested, its antigenic epitope was intact, and could be
    bound and immobilized by the anti-HBsAg in the strip.
    We therefore concluded that this method is good enough to prepare the HBsAg.

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