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    Lab Report 1 Biology Photosynthesis

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    Sarthak Patel
    This lab report examines the effect of light and carbon dioxide on the rate of photosynthesis in spinach leaf disks. The experiment measures the rate that leaf disks float in a bicarbonate solution under light, as photosynthesis produces oxygen that allows the disks to rise. It also measures the rate disks sink in darkness and without carbon dioxide, representing cellular respiration. Results show disks floated faster with light and carbon dioxide present. A second trial with a farther light source slowed the floating rate. The report concludes the experiment supported the hypotheses that light and carbon dioxide increase photosynthesis rates.

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    Lab Report 1 Biology Photosynthesis

    Uploaded by

    Sarthak Patel
    1K views4 pages
    This lab report examines the effect of light and carbon dioxide on the rate of photosynthesis in spinach leaf disks. The experiment measures the rate that leaf disks float in a bicarbonate solution under light, as photosynthesis produces oxygen that allows the disks to rise. It also measures the rate disks sink in darkness and without carbon dioxide, representing cellular respiration. Results show disks floated faster with light and carbon dioxide present. A second trial with a farther light source slowed the floating rate. The report concludes the experiment supported the hypotheses that light and carbon dioxide increase photosynthesis rates.

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    Photosynthesis

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    This lab report examines the effect of light and carbon dioxide on the rate of photosynthesis in spinach leaf disks. The experiment measures the rate that leaf disks float in a bicarbonate solution under light, as photosynthesis produces oxygen that allows the disks to rise. It also measures the rate disks sink in darkness and without carbon dioxide, representing cellular respiration. Results show disks floated faster with light and carbon dioxide present. A second trial with a farther light source slowed the floating rate. The report concludes the experiment supported the hypotheses that light and carbon dioxide increase photosynthesis rates.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    1K views4 pages

    Lab Report 1 Biology Photosynthesis

    Uploaded by

    Sarthak Patel
    This lab report examines the effect of light and carbon dioxide on the rate of photosynthesis in spinach leaf disks. The experiment measures the rate that leaf disks float in a bicarbonate solution under light, as photosynthesis produces oxygen that allows the disks to rise. It also measures the rate disks sink in darkness and without carbon dioxide, representing cellular respiration. Results show disks floated faster with light and carbon dioxide present. A second trial with a farther light source slowed the floating rate. The report concludes the experiment supported the hypotheses that light and carbon dioxide increase photosynthesis rates.

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    Biol 1108

    Section 001
    2/23/15
    Lab Report 1: Photosynthesis Lab
    Effect of ______ on ___ and
    Introduction:
    Photosynthesis and cellular respiration are important biochemical life processes that
    involve a series of catalytic reactions. Photoautotrophs are able to convert of inorganic carbon
    dioxide to organic, high-energy glucose using light energy in their chloroplasts (EEB dept.
    2014). In comparison cellular respiration happens in the cytoplasm of bacteria and in the
    mitochondria of eukaryotic cells where high energy glucose is broken down in order to capture
    energy as ATP molecules.
    This experiment will seek to examine rate of photosynthesis and respiration in a spinach leaf
    tissue using leaf disks. We predict that under light conditions, the leaf discs will display
    photosynthesis and under dark conditions the leaf discs will show cellular respiration. In
    addition, we hypothesize that if the lighting apparatus is moved farther away from the leaf discs
    then the rate of photosynthesis will be observably slower.
    -- what is biological reason?
    Photosynthesis
    Light Energy

    6CO2 + 6H2O C6H12O6 + 6O2

    Cellular Respiration
    C6H12O6 + 6O2 6CO2 + 6H2O + ATP

    Materials and Methods:


    Spinach leaves were punched out in order to create leaf discs that were initially placed in
    a syringe barrel with the plunger removed. The plunger was replaced and slowly pushed towards
    the tip in order to remove air after which 3 cc of 0.2% bicarbonate/detergent solution was added
    to the syringe as a CO2 source for the leaf discs. A vacuum was created inside the syringe and the
    discs were allowed to sink and then poured into a plastic cup containing bicarbonate solution.
    The cup with the discs was placed under a light source and the number of disks floating at the
    surface was recorded every minute until all discs are floating. As photosynthesis occurs in the
    presence of CO2 and light, oxygen will be produced and discs will expand and rise. After all discs
    have risen the cup will be placed in a dark cabinet, and the rate at which the leaf discs sink will
    be used to measure cellular respiration. In the control experiment, the plastic cup consisted of
    distilled water in order to represent the absence of CO2 and the rate of photosynthesis was
    measured. In addition, a second experiment was performed where the lighting apparatus was
    heightened and placed farther away from the leaf discs and the effect on the rate of
    photosynthesis was analyzed.

    PSET50:

    Figure 1. Observed floating discs out of 15 in the presence and absence of light.

    Figure 2. Observed floating discs out of 19 in distilled water and a heightened light source.

    Module 1: The Abosrpiton Spectra of Plant Pigments


    Module 3: Floating Leaf Disk Assay
    Results:

    Discussion:
    References: need 2
    Department of Ecology and Evolutionary Biology, University of Connecticut. Biology 1108
    Laboratory Manual, 2014 edition, University of Connecticut, Storrs, Connecticut.

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